scholarly journals Phytochemical Profiling of Medicinal Plants Extracts and Their Antioxidant and Anticancer Potentialities Against Human Liver Cancer (Hep G2) Cell Lines

2021 ◽  
Vol 72 (1) ◽  
pp. 100-110
Author(s):  
Nusrat Shafiq ◽  
Farah Yasmin ◽  
Sadia Noreen ◽  
Alina Shahzad ◽  
Zill-I-huma Nazli ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Liver Cancer ◽  
Secondary Metabolites ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Human Liver ◽  
Biological Effects ◽  
Hep G2 ◽  
Human Liver Cancer

Although Pakistan is stacked with enormous varieties of medicinal plants, only a little proportion of these plants has been evaluated for their medicinal and therapeutic properties. Herein, four indigenous medicinal plants Citrus sinenesis, Citrus paradiasii, Moringa olifera, and Hibiscus rosa-sinenesis were collected and subjected to phytochemical analyses to scrutinize the presence of secondary metabolites. Qualitative analy�sis showed the presence of an array of secondary metabolites in the selected plants, which were further corroborated by high-performance liquid chromatography. Results revealed the presence of 33.24, 21.04, 15.2 ppm gallic acid in methanol, ethyl acetate and n-hexane fraction of C. sinensis peels extract, respectively. C. paradaissi peels consist of 24.06, and 18.24 ppm of gallic acid and caffeic acid, respectively, in methanol and chloroform fractions, whereas its methanolic seeds extract contain caffeic acid as a major component (10.63 ppm). H. rosa-sinenesis has shown p-coumaric acid, caffeic acid, and gallic acid at 35.26, 15.04, and 11.4 ppm, respectively. M. olifera contained 3.24 ppm gallic acid in pods extract while stems and leaves extract contain a very low amount. Anticancer profile evinced that Citrus sinensis extract showed the highest percent inhibition (142.746%) of human liver cancer (Hep G2) cell lines followed by H. rosa-sinensis (132.49%), C. paradaisii (82.39%) and M. olifera (68.0%). The determined IC50 values for antioxidant activity were C. sinenesis (IC50=0.49 mM), C. paradaisii (IC50=0.43 mM), M. olifera (IC50=0.42 mM) and H. rosa-sinensis (IC50=0.41 mM). Conclusively, the selected plants could be an effective alternative and deliverable chemical therapeutic to the pharmaceutical industry due to their excellent biological effects.

scholarly journals Antiproliferative Effect of Lenvatinib on Human Liver Cancer Cell Lines In Vitro and In Vivo

2019 ◽  
Vol 39 (11) ◽  
pp. 5973-5982 ◽  
Author(s):  
SACHIKO OGASAWARA ◽  
YUTARO MIHARA ◽  
REIICHIRO KONDO ◽  
HIRONORI KUSANO ◽  
JUN AKIBA ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Liver ◽  
Antiproliferative Effect ◽  
Liver Cancer Cell ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

scholarly journals A Titanium (IV)–Dithiophenolate Complex and Its Chitosan Nanocomposite: Their Roles towards Rat Liver Injuries In Vivo and against Human Liver Cancer Cell Lines

2021 ◽  
Vol 22 (20) ◽  
pp. 11219
Author(s):  
Nadia Z. Shaban ◽  
Salah A. Yehia ◽  
Doaa Awad ◽  
Shaban Y. Shaban ◽  
Samar R. Saleh
Keyword(s):  
Oxidative Stress ◽  
Liver Cancer ◽  
Cell Lines ◽  
Rat Liver ◽  
Liver Damage ◽  
Human Liver ◽  
Therapeutic Effects ◽  
Liver Injuries ◽  
Human Liver Cancer

Titanium (IV)–dithiophenolate complex chitosan nanocomposites (DBT–CSNPs) are featured by their antibacterial activities, cytotoxicity, and capacity to bind with DNA helixes. In this study, their therapeutic effects against rat liver damage induced by carbon tetrachloride (CCl4) and their anti-proliferative activity against human liver cancer (HepG2) cell lines were determined. Results of treatment were compared with cisplatin treatment. Markers of apoptosis, oxidative stress, liver functions, and liver histopathology were determined. The results showed that DBT–CSNPs and DBT treatments abolished liver damage induced by CCl4 and improved liver architecture and functions. DNA fragmentation, Bax, and caspase-8 were reduced, but Bcl-2 and the Bcl-2/Bax ratios were increased. However, there was a non-significant change in the oxidative stress markers. DBT–CSNPs and DBT inhibited the proliferation of HepG2 cells by arresting cells in the G2/M phase and inducing cell death. DBT–CSNPs were more efficient than DBT. Low doses of DBT and DBT–CSNPs applied to healthy rats for 14 days had no adverse effect. DBT and DBT–CSNP treatment gave preferable results than the treatment with cisplatin. In conclusion, DBT–CSNPs and DBT have anti-apoptotic activities against liver injuries and have anti-neoplastic impacts. DBT–CSNPs are more efficient. Both compounds can be used in pharmacological fields.

scholarly journals Mechanism of action of novel piperazine containing compound toxicant against human liver cancer cells

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1588 ◽  
Author(s):  
Nima Samie ◽  
Sekaran Muniandy ◽  
MS Kanthimathi ◽  
Batoul Sadat Haerian
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Liver ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

The purpose of this study was to assess the cytotoxic potential of a novel piperazine derivative (PCC) against human liver cancer cells. SNU-475 and 423 human liver cancer cell lines were used to determine the IC50 of PCC using the standard MTT assay. PCC displayed a strong suppressive effect on liver cancer cells with an IC50 value of 6.98 ± 0.11 µM and 7.76 ± 0.45 µM against SNU-475 and SNU-423 respectively after 24 h of treatment. Significant dipping in the mitochondrial membrane potential and elevation in the released of cytochrome c from the mitochondria indicated the induction of the intrinsic apoptosis pathway by PCC. Activation of this pathway was further evidenced by significant activation of caspase 3/7 and 9. PCC was also shown to activate the extrinsic pathways of apoptosis via activation of caspase-8 which is linked to the suppression of NF-κB translocation to the nucleus. Cell cycle arrest in the G1 phase was confirmed by flow cytometry and up-regulation of glutathione reductase expression was quantified by qPCR. Results of this study suggest that PCC is a potent anti-cancer agent inducing both intrinsic and extrinsic pathways of apoptosis in liver cancer cell lines.

Allicin Induces p53-Mediated Autophagy in Hep G2 Human Liver Cancer Cells

2012 ◽  
Vol 60 (34) ◽  
pp. 8363-8371 ◽  
Author(s):  
Yung-Lin Chu ◽  
Chi-Tang Ho ◽  
Jing-Gung Chung ◽  
Raghu Rajasekaran ◽  
Lee-Yan Sheen
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Hep G2 ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells

scholarly journals HNF1α inhibition triggers epithelial-mesenchymal transition in human liver cancer cell lines

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Laura Pelletier ◽  
Sandra Rebouissou ◽  
Danijela Vignjevic ◽  
Paulette Bioulac-Sage ◽  
Jessica Zucman-Rossi
Keyword(s):  
Liver Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Liver ◽  
Epithelial Mesenchymal Transition ◽  
Cancer Cell Lines ◽  
Liver Cancer Cell ◽  
Mesenchymal Transition ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer
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