scholarly journals Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)

2021 ◽  
Vol 80 (2) ◽  
Author(s):  
Ergun Kaya ◽  
Selin Galatali ◽  
Irem Aktay ◽  
Onur Celik ◽  
Bilge Ozturk ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Kernel Method ◽  
Eastern Mediterranean ◽  
Medium Composition ◽  
Regeneration Rate ◽  
Graph Kernel ◽  
Proliferation Medium ◽  
Shoot Number

Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

scholarly journals Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition

2014 ◽  
Vol 22 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Rossen S. Sokolov ◽  
Bistra Y. Atanassova ◽  
Elena T. Iakimova
Keyword(s):  
Nutrient Medium ◽  
Culture Conditions ◽  
Medium Composition ◽  
Naphthaleneacetic Acid ◽  
Axillary Shoots ◽  
Regeneration Rate ◽  
Primary Explants ◽  
High Regeneration ◽  
Basal Salts

AbstractThe objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity.

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals In vitro propagation of Passiflora edulis through internodal segments as affected by medium composition

2021 ◽  
Vol 108 (4) ◽  
pp. 377-382
Author(s):  
Natalija Burbulis ◽  
Aušra Blinstrubienė ◽  
Aistis Petruškevičius
Keyword(s):  
In Vitro Propagation ◽  
Medium Composition ◽  
Passiflora Edulis

scholarly journals Micropropagation, rooting, and acclimatization of two cultivars of goji (Lycium chinense)

2021 ◽  
Vol 49 (2) ◽  
pp. 12271
Author(s):  
Arleta KRUCZEK ◽  
Marcelina KRUPA-MAŁKIEWICZ ◽  
Ireneusz OCHMIAN
Keyword(s):  
Shoot Length ◽  
Public Acceptance ◽  
Ms Medium ◽  
Lycium Chinense ◽  
Regeneration Rate ◽  
Health Value ◽  
Shoot Number ◽  
Rapid Production ◽  
The Mean

In recent years, Lycium chinense (goji) has become increasing popular because of its public acceptance as a “superfood”. Hence, the present study aimed to develop a rapid production technology by using in vitro culture to produce plants with high health value, throughout year and in desired quantities. A micropropagation protocol for growing L. chinense ‘No 1’ and ‘New Big’ cultivars was developed. The explants were grown on MS medium supplemented with different concentrations of meta-Topolin (0.4-0.8 mg L-1), and WPM and RA without plant hormones. Among the tested combinations, the maximum regeneration rate (95-97%) with the mean shoot length of 3.53-4.12 cm and mean shoot number of 1.42–1.58 (‘No 1’ and ‘New Big’, respectively) was recorded for plants grown on MS with 0.6 mg L-1 mT and WPM. For in vitro rooting, healthy roots (4.71-4.91 cm) were obtained on MS with the addition of 20 ppm chitosan. A maximum of 70–80% plantlets (‘No 1’ and ‘New Big’, respectively) regenerated on the medium with chitosan were successfully acclimatized and established in the mixture of 90% peat and 10% perlite under field conditions.

The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] Assay Is a Fast and Reliable Method for Colorimetric Determination of Fungal Cell Densities

1999 ◽  
Vol 65 (8) ◽  
pp. 3727-3729 ◽  
Author(s):  
Florian M. Freimoser ◽  
Claude A. Jakob ◽  
Markus Aebi ◽  
Urs Tuor
Keyword(s):  
Colorimetric Method ◽  
Medium Composition ◽  
Colorimetric Determination ◽  
Fungal Cell ◽  
Diphenyltetrazolium Bromide ◽  
Hyphal Bodies ◽  
Growth Promoting ◽  
Cell Densities

ABSTRACT The entomopathogenic fungus Neozygites parvispora(Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.

scholarly journals Modeling and Optimizing Medium Composition for Shoot Regeneration of Chrysanthemum via Radial Basis Function-Non-dominated Sorting Genetic Algorithm-II (RBF-NSGAII)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohsen Hesami ◽  
Roohangiz Naderi ◽  
Masoud Tohidfar
Keyword(s):  
Genetic Algorithm ◽  
Sensitivity Analysis ◽  
Radial Basis Function ◽  
Basis Function ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Validation Data ◽  
Shoot Number ◽  
Radial Basis

AbstractThe aim of the current study was modeling and optimizing medium compositions for shoot proliferation of chrysanthemum, as a case study, through radial basis function- non-dominated sorting genetic algorithm-II (RBF-NSGAII). RBF as one of the artificial neural networks (ANNs) was used for modeling four outputs including proliferation rate (PR), shoot number (SN), shoot length (SL), and basal callus weight (BCW) based on four variables including 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), phloroglucinol (PG), and sucrose. Afterward, models were linked to the optimization algorithm. Also, sensitivity analysis was applied for evaluating the importance of each input. The R2 correlation values of 0.88, 0.91, 0.97, and 0.76 between observed and predicted data were obtained for PR, SN, SL, and BCW, respectively. According to RBF-NSGAII, optimal PR (98.85%), SN (13.32), SL (4.83 cm), and BCW (0.08 g) can be obtained from a medium containing 2.16 µM BAP, 0.14 µM IBA, 0.29 mM PG, and 87.63 mM sucrose. The results of sensitivity analysis indicated that PR, SN, and SL were more sensitive to BAP, followed by sucrose, PG, and IBA. Finally, the performance of predicted and optimized medium compositions were tested, and results showed that the difference between the validation data and RBF-NSGAII predicted and optimized data were negligible. Generally, RBF-NSGAII can be considered as an efficient computational strategy for modeling and optimizing in vitro organogenesis.

Copper and Zinc Induced Amelioration of In vitro Multiplication of Dendrocalamus strictus (Roxb.) Nees

2017 ◽  
Vol 40 (2) ◽  
pp. 181-184
Author(s):  
Manish Singh ◽  
◽  
Manpreet Kaur ◽  
Meena Bakshi ◽  
Satakshi Kapurwan ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Shoot Length ◽  
Ms Medium ◽  
Forest Resource ◽  
Similar Concentration ◽  
Dendrocalamus Strictus ◽  
Copper And Zinc ◽  
Shoot Number ◽  
Induced Amelioration

Bamboos are among most significant plants worldwide and economically important forest resource in many countries of Asia. In vitro propagation is a potential and well known technique for mass multiplication of bamboo. Efforts were made for multiplication of Dendrocalamus strictus through aseptic nodal explants and its acceleration through heavy metal infusion. A rapid in vitro propagation protocol was established. Maximum germination with 100% survival rate was recorded in MS media supplemented with 1.0 mg/l BAP in combination with TDZ (0.25 mg/l) with a maximum shoot number of 9.36±0.67 and shoot length of 6.85 ± 0.95. The effects of copper and zinc were assessed on morphogenesis. The specific concentration of copper and zinc in MS medium were doubled (2x), quintuple pentadrupled (5x) and decupled (10x). The results revealed that maximum mean shoot number and mean shoot length was observed in quintuple pentadrupled zinc i.e. 6.80 ± 0.74 and 6.60 ± 0.80 cm respectively, however as concerned to copper, maximum shoot number (5.56 ±0.92) and shoot length (5.87 ± 0.78) were observed at similar concentration. Although copper and zinc are toxic for plants at higher concentration, but a good in vitro propagation response was observed in quintuple pentadrupled concentration.

Requirement for sorbitol (D-glucitol) as carbon source for in vitro propagation of Malus robusta No. 5

1984 ◽  
Vol 62 (7) ◽  
pp. 1545-1549 ◽  
Author(s):  
E.-C. Pua ◽  
Calvin Chong
Keyword(s):  
Carbon Source ◽  
Nutrient Medium ◽  
In Vitro Propagation ◽  
Agar Medium ◽  
Shoot Cultures ◽  
Indolebutyric Acid ◽  
Shoot Number ◽  
Shoot Weight ◽  
Number Of Shoots

Meristem tip explants of Malus robusta Rehd. No. 5 were successfully initiated in vitro on nutrient medium with sorbitol but not with glucose, sucrose, or fructose, each supplied at 30 g/L. Cultures, subsequently subcultured and multiplied on sorbitol medium, produced the highest number of shoots and had the highest shoot fresh and dry weights, followed in descending order by cultures grown on glucose, sucrose, and fructose media. Decreasing the proportion of sorbitol and increasing that of sucrose in the carbon source fraction (30 g/L total) resulted in decreasing shoot number and shoot weight accompanied by increasing leaf/stem ratio and compactness of the shoot cultures. After 32 days on agar medium with 0.5 mg/L indolebutyric acid in the presence of either sorbitol or sucrose, 85% rooting was achieved.

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