Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

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Anti-Inflammatory Effect of Asterias amurensis Fatty Acids through NF-��B and MAPK Pathways against LPS-Stimulated RAW264.7 Cells

Journal of Microbiology and Biotechnology ◽

10.4014/jmb.1802.03044 ◽

2018 ◽

Vol 28 (10) ◽

pp. 1635-1644 ◽

Cited By ~ 3

Author(s):

Chaiwat Monmai ◽

Seok Hyeon Go ◽

Il-shik Shin ◽

SangGuan You ◽

Dae-ok Kim ◽

...

Keyword(s):

Fatty Acids ◽

Raw264.7 Cells ◽

Asterias Amurensis ◽

Mapk Pathways ◽

Anti Inflammatory ◽

Inflammatory Effect

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Anti-Inflammatory Effect of the Extracts from Abeliophyllum distichum Nakai in LPS-Stimulated RAW264.7 Cells

Korean Journal of Plant Resources ◽

10.7732/kjpr.2014.27.3.209 ◽

2014 ◽

Vol 27 (3) ◽

pp. 209-214 ◽

Cited By ~ 7

Author(s):

Gwang Hun Park ◽

Jae Ho Park ◽

Hyun Ji Eo ◽

Hun Min Song ◽

Man Hyo Lee ◽

...

Keyword(s):

Raw264.7 Cells ◽

Anti Inflammatory ◽

Abeliophyllum Distichum ◽

Inflammatory Effect

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Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

BioMed Research International ◽

10.1155/2013/814693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Cuauhtemoc Pérez González ◽

Roberto Serrano Vega ◽

Marco González-Chávez ◽

Miguel Angel Zavala Sánchez ◽

Salud Pérez Gutiérrez

Keyword(s):

Medicinal Plant ◽

Methanol Extract ◽

Chloroform Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Active Fraction ◽

Ear Edema ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

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Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema

Microbiology and Biotechnology Letters ◽

10.4014/mbl.1610.10002 ◽

2017 ◽

Vol 45 (2) ◽

pp. 110-117 ◽

Cited By ~ 2

Author(s):

Min-Ji Kim ◽

Min-Ju Kim ◽

Koth Bong Woo Ri Kim ◽

Sun Hee Park ◽

Hyeun Deok Choi ◽

...

Keyword(s):

Ethanol Extract ◽

Raw264.7 Cells ◽

Ear Edema ◽

Mouse Ear ◽

Anti Inflammatory ◽

Sargassum Patens ◽

Mouse Ear Edema ◽

Inflammatory Effect

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MiR-155/GSK-3β mediates anti-inflammatory effect of Chikusetsusaponin IVa by inhibiting NF-κB signaling pathway in LPS-induced RAW264.7 cell

Scientific Reports ◽

10.1038/s41598-020-75358-1 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Yi Xin ◽

Qin Yuan ◽

Chaoqi Liu ◽

Changcheng Zhang ◽

Ding Yuan

Keyword(s):

Signaling Pathway ◽

Mouse Liver ◽

Inflammatory Responses ◽

Biological Activities ◽

Raw264.7 Cells ◽

Inflammation Model ◽

Raw264.7 Cell ◽

Anti Inflammatory ◽

Gsk 3Β ◽

Inflammatory Effect

Abstract It has been demonstrated that Chikusetsusaponin IVa (CsIVa) possesses abundant biological activities. Herein, using LPS to establish acute inflammation model of mouse liver and cell line inflammation model, we investigated whether miR-155/GSK-3β regulated NF-κB signaling pathway, and CsIVa exerted anti-inflammatory effects by regulating miR-155/GSK-3β signaling pathway. Our results showed that LPS induced high expression of miR-155 and miR-155 promoted macrophage activation through GSK-3β. In addition, CsIVa inhibited inflammatory responses in LPS-induced mouse liver and RAW264.7 cells. Furthermore, we demonstrated that CsIVa improved the inflammatory response in LPS-induced RAW264.7 cells by inhibiting miR-155, increasing GSK-3β expression, and inhibiting NF-κB signaling pathway. In conclusion, our study reveals that CsIVa suppresses LPS-triggered immune response by miR-155/GSK-3β-NF-κB signaling pathway.

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