Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

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Anti-Inflammatory Activity and Composition ofSenecio salignusKunth

BioMed Research International ◽

10.1155/2013/814693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

Cuauhtemoc Pérez González ◽

Roberto Serrano Vega ◽

Marco González-Chávez ◽

Miguel Angel Zavala Sánchez ◽

Salud Pérez Gutiérrez

Keyword(s):

Medicinal Plant ◽

Methanol Extract ◽

Chloroform Extract ◽

Inflammatory Activity ◽

Paw Edema ◽

Active Fraction ◽

Ear Edema ◽

Methanol Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

We investigated the anti-inflammatory activity ofSenecio salignus. This medicinal plant is often used in Mexico for the treatment of fever and rheumatism. Chloroform and methanol extracts of the plant were tested on 12-O-tetradecanoylphorbol-13-acetate- (TPA-) induced edema in mice ears. The methanol extract of the plant inhibited edema by36±4.4% compared with the control, while the chloroform extract exhibited an even greater level of inhibition (64.1%). The chloroform extract was then fractionated, and the composition of the active fraction was determined by GC-MS. The anti-inflammatory activity of this fraction was then tested on TPA-induced ear edema in mice, and we found that the active fraction could inhibit edema by 46.9%. The anti-inflammatory effect of the fraction was also tested on carrageenan-induced paw edema in rats at doses of 100 mg/kg; a58.9±2.8% reduction of the edema was observed 4 h after administration of carrageenan, and the effect was maintained for 5 h.

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SPASMOLYTIC, ANTI-INFLAMMATORY, AND ANTIOXIDANT ACTIVITY OF SALVIA GESNERIFLORA LINDLEY

African Journal of Traditional Complementary and Alternative Medicines ◽

10.21010/ajtcam.v15i4.10 ◽

2018 ◽

Vol 15 (4) ◽

pp. 72

Author(s):

Abraham Gómez-Rivera ◽

Manasés González-Cortazar ◽

Ammy Joana Gallegos-García ◽

Armando Escobar-Ramos ◽

Gabriel Flores-Franco ◽

...

Keyword(s):

Antioxidant Activity ◽

Guinea Pig ◽

Ursolic Acid ◽

Antioxidant Potential ◽

Guinea Pig Ileum ◽

Chromatographic Profile ◽

Mouse Ear ◽

Inflammatory Edema ◽

Anti Inflammatory

Background: Salvia gesneriflora Lindley is employed in traditional Mexican medicine for the treatment of several diseases. Materials and Methods: The chromatographic profile of three extracts (SgH, SgD and SgM) of this plant allowed the identification of 11 components in SgH, the presence of rosmarinic (1), chlorogenic (2) and caffeic (3) acids and quercetin glucoside (4) in SgM and of ursolic acid (5) in SgD. The spasmolytic (electrically induced contractions of guinea-pig ileum), anti-inflammatory (edema in mouse ear) and antioxidant potential (DPPH, ABTS and FRAP assays) of the extracts were evaluated. Results: SgM showed the highest percentage of relaxation (80.67 ± 1.633%) with no significant difference (p

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In vitro antioxidant, anti-inflammatory and anti-cancer activities of methanolic extract of Asparagus horridus grows in North Cyprus Kuzey Kıbrıs da yetişen Asparagus horridus metanolik ekstraktının in-vitro antioksidan, anti-enflamatuar ve anti-kanser aktivitesi

Turkish Journal of Biochemistry ◽

10.1515/tjb-2019-0325 ◽

2020 ◽

Vol 45 (4) ◽

pp. 365-372

Author(s):

Ergul Mutlu Altundag ◽

Duygu Gençalp ◽

Cahit Özbilenler ◽

Kübra Toprak ◽

Namık Kerküklü

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Protein Denaturation ◽

Soxhlet Extraction ◽

Edible Plant ◽

North Cyprus ◽

Anti Cancer ◽

Anti Inflammatory ◽

Inflammatory Effect

AbstractBackgroundAsparagus horridus is an edible plant known as “Ayrelli” in North Cyprus. The scientific literature has not yet submitted a report about the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus plant from North Cyprus until now. The purpose of the research was to determine the antioxidant, anti-inflammatory and anti-cancer activities of A. horridus.Materials and methodsSoxhlet extraction of A. horridus was performed using methanol. Antioxidant activity was determined by DPPH, TFC, FRAP and TPC assays. Protein-denaturation assay was performed to determine the anti-inflammatory effect. The anti-cancer effects of the extract on HepG2 and B-CPAP cell lines were determined with MTT assay.ResultsAntioxidant activity for A. horridus extract was determined by DPPH (50%), TFC (266.26 μg QUE/mg extract), FRAP (1.27 μg FeSO4/mg extract) and TPC (167.613 μg GAE/mg extract) assays at 25 mg/mL. Inhibition of protein-denaturation activity was found as 29.42% at 25 mg/mL. After 24 h of the extract treatment, cell proliferation of HepG2 and B-CPAP cancer cells were inhibited at IC50 values 63.24 μg/mL and 101.24 μg/mL, respectively.ConclusionThese results have shown that the methanol extract of A. horridus grows in North Cyprus has antioxidant, anti-inflammatory and anti-cancer activities.

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Antagonistic effect of corynanatheidine, an alkaloid from Thai medicinal plant Mitragyna speciosa, on opioid receptors in guinea-pig ileum

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)40351-x ◽

1998 ◽

Vol 76 ◽

pp. 58

Author(s):

Tomoyuki Moriyama ◽

Leonardo T. Yamamoto ◽

Syunji Horie ◽

Shingo Yano ◽

Hiromitsu Takayama ◽

...

Keyword(s):

Medicinal Plant ◽

Guinea Pig ◽

Opioid Receptors ◽

Antagonistic Effect ◽

Guinea Pig Ileum ◽

Mitragyna Speciosa

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Anti-inflammatory study on crude methanol extract and different fractions ofEremostachys laciniata

Pharmaceutical Biology ◽

10.3109/13880200903517950 ◽

2010 ◽

Vol 48 (10) ◽

pp. 1115-1118 ◽

Cited By ~ 4

Author(s):

Salimullah Khan ◽

Muhammad Nisar ◽

Wajid Rehman ◽

Razaullah Khan ◽

Fazli Nasir

Keyword(s):

Methanol Extract ◽

Anti Inflammatory ◽

Crude Methanol Extract

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Pharmacognostic Screening and Anti-inflammatory Investigation of the Methanol extract of stem bark of Blighia unijugata Baker (Sapindaceae)

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i4.4167 ◽

2020 ◽

Vol 10 (4) ◽

pp. 146-152

Author(s):

Felix N. Osuala ◽

Uchenna E. Odoh ◽

VictoriaC. Onuigbo ◽

Sylvester C. Ohadoma

Keyword(s):

Methanol Extract ◽

Lethal Dose ◽

Numerical Data ◽

Stem Bark ◽

Water Soluble ◽

Herbal Remedies ◽

Albino Rats ◽

Standard Drug ◽

Anti Inflammatory ◽

Inflammatory Effect

Introduction: Blighia unijugata is a potent medicinal plant extensively employed in traditional herbal remedies for the treatment of various diseases such as fever, fertility, inflammation, hypertension, migraine and treatment of infections due to microorganisms. Aim: This work, investigates the pharmacognostic screening and anti-inflammatory activities of the methanol extract of B. unijugata stem bark. Method: Collection, drying, pulverization, and methanol extraction of the stem bark were done accordingly. The screening of phytochemical constituents and Pharmacognostic numerical data were carried out. The chromatographic analysis was carried out using TLC. The acute toxicity was determined using Lorke’s method. Methanol extract was investigated for anti-inflammatory effect in albino rats using egg-induced hind paw oedema at doses of 200, 400 and 600mg/kg body weight respectively. Result: The macroscopical investigation showed the stem bark outer layer is greyish and, the inner layer is pale reddish brown, disagreeable odour, bitter, Microscopical screening revealed the presence of starch grains, trichomes and sclerenchyma cells. Chemomicroscopic result revealed the presence of lignin, starch, calcium oxalate, cellulose, stone cells. Qualitative phytochemical screening of the powdered bark showed the presence of saponins, steroids, tannins, and resins. Numerical data: moisture content/ weight loss on drying gave value of 7.7%, percentage yield of 6.7%, alcoholic soluble extractive 5.6%, water soluble extractive 6.3%, total ash of 7.1%, acid insoluble ash 0.57% and water soluble ash 4.56%. The chromatographic screening results were close compared with the standard drug. Toxicity test established the lethal dose of greater than 5000mg/kg. There was a significant inhibition of the edema p>0.05, the presence of various bioactive constituents may have contributed to the anti-inflammatory properties of the plant extract. Conclusion: The result of this study confirms that the barks of B. unijugata have anti-inflammatory effect and justifies the use as traditional treatment of inflammation and pain. Keywords: Blighia unijugata, Inflammation, Inflammatory agents, Anti inflammatory

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Anti-Inflammatory Effects of Huberia peruviana Cogn. Methanol Extract by Inhibiting Src Activity in the NF-κB Pathway

Plants ◽

10.3390/plants10112335 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2335

Author(s):

Seung A Kim ◽

Chae Young Lee ◽

Ankita Mitra ◽

Haeyeop Kim ◽

Byoung Young Woo ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Western Blotting ◽

Methanol Extract ◽

Luciferase Assay ◽

Luciferase Reporter ◽

No Production ◽

In Vivo Models ◽

Anti Inflammatory ◽

Inflammatory Effect

There is a growing need to develop anti-inflammatory drugs to regulate inflammatory responses. An extract of Huberia peruviana Cogn. had the best inhibitory effect on nitric oxide (NO) production in screening process undertaken in our laboratory. However, the anti-inflammatory effect of Huberia peruviana Cogn. methanol extract (Hp-ME) has not been studied. In this study, the anti-inflammatory effect of Hp-ME was assessed by using an NO assay, RT-PCR, luciferase reporter gene activity assay, western blotting assay, HCl/EtOH-induced acute gastritis model, and LPS-induced acute lung injury model. The phytochemical components of Hp-ME were determined through LC-MS/MS analysis. When RAW264.7 and HEK293T cells were treated with Hp-ME, NO production was decreased dose-dependently without cytotoxicity and the mRNA levels of iNOS, COX-2, and TNF-α were decreased. In a luciferase assay, the activity of transcription factors, NF-κB in TRIF or MyD88-overexpressing HEK293T cells was extremely reduced by Hp-ME. The western blotting analysis indicated that Hp-ME has anti-inflammatory effects by inhibiting the phosphorylation of Src. Hp-ME showed anti-inflammatory effects on in vivo models of HCl/EtOH-induced gastritis and LPS-induced acute lung injury. LC-MS/MS revealed that Hp-ME contains several anti-inflammatory flavonoids. The final findings of this study imply that Hp-ME could be used as an anti-inflammatory drug in several inflammatory diseases.

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