scholarly journals New NCI-N87-derived human gastric epithelial line after human telomerase catalytic subunit over-expression

2015 ◽  
Vol 21 (21) ◽  
pp. 6526 ◽  
Author(s):  
Kathy Saraiva-Pava
Keyword(s):  
Catalytic Subunit ◽  
Over Expression ◽  
Human Telomerase

scholarly journals Apoptotic endonuclease EndoG regulates alternative splicing of human telomerase catalytic subunit hTERT

2016 ◽  
Vol 62 (5) ◽  
pp. 544-554 ◽  
Author(s):  
D.D. Zhdanov ◽  
D.A. Vasina ◽  
E.V. Orlova ◽  
V.S. Orlova ◽  
M.V. Pokrovskaya ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Telomerase Activity ◽  
Catalytic Subunit ◽  
Over Expression ◽  
Htert Gene ◽  
Template Strand ◽  
Non Coding Rna ◽  
Dna Strand ◽  
Human Telomerase ◽  
Long Non Coding Rna

Human telomerase catalytic subunit hTERT is subjected to alternative splicing results in loss of its function and leads to decrease of telomerase activity. However, very little is known about the mechanism of hTERT pre-mRNA alternative splicing. Apoptotic endonuclease EndoG is known to participate this process. The aim of this study was to determine the role of EndoG in regulation of hTERT alternative splicing. Increased expression of b-deletion splice variant was determined during EndoG over-expression in CaCo-2 cell line, after EndoG treatment of cell cytoplasm and nuclei and after nuclei incubation with EndoG digested cell RNA. hTERT alternative splicing was induced by 47-mer RNA oligonucleotide in naked nuclei and in cells after transfection. Identified long non-coding RNA, that is the precursor of 47-mer RNA oligonucleotide. Its size is 1754 nucleotides. Based on the results the following mechanism was proposed. hTERT pre-mRNA is transcribed from coding DNA strand while long non-coding RNA is transcribed from template strand of hTERT gene. EndoG digests long non-coding RNA and produces 47-mer RNA oligonucleotide complementary to hTERT pre-mRNA exon 8 and intron 8 junction place. Interaction of 47-mer RNA oligonucleotide and hTERT pre-mRNA causes alternative splicing.

scholarly journals Ectopic Human Telomerase Catalytic Subunit Expression Maintains Telomere Length But Is Not Sufficient for CD8+T Lymphocyte Immortalization

2000 ◽  
Vol 165 (9) ◽  
pp. 4978-4984 ◽  
Author(s):  
Marco Migliaccio ◽  
Mario Amacker ◽  
Tom Just ◽  
Patrick Reichenbach ◽  
Danila Valmori ◽  
...  
Keyword(s):  
Telomere Length ◽  
T Lymphocyte ◽  
Catalytic Subunit ◽  
Cd8 T Lymphocyte ◽  
Subunit Expression ◽  
Human Telomerase

scholarly journals Induction of apoptotic endonuclease EndoG with DNA-damaging agents initiates alternative splicing of telomerase catalytic subunit hTERT and inhibition of telomerase activity hTERT in human CD4+ and CD8+ T-lymphocytes

2017 ◽  
Vol 63 (4) ◽  
pp. 296-305 ◽  
Author(s):  
D.D. Zhdanov ◽  
D.A. Vasina ◽  
V.S. Orlova ◽  
E.V. Orlova ◽  
D.V. Grishin ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
T Lymphocytes ◽  
Splice Variants ◽  
Telomerase Activity ◽  
Catalytic Subunit ◽  
Human Telomerase Reverse Transcriptase ◽  
Dna Damages ◽  
Dna Damaging Agents ◽  
Cd8 T Lymphocytes ◽  
Human Telomerase

Activity of telomerase catalytic subunit hTERT (human Telomerase Reverse Transcriptase) can be regulated by alternative splicing of its mRNA. At present time exact mechanism of hTERT splicing is not fully understood. Apoptotic endonuclease EndoG is known to participate this process. EndoG expression is induced by DNA damages. The aim of this work was to investigate the ability of DNA-damaging agents with different mechanism of action to induce EndoG expression and inhibit telomerase activity due to the activation of hTERT alternative splicing in normal activated human CD4+ and CD8+ T-lymphocytes. All investigated DNA-damaging agents were able to induce EndoG expression. Cisplatin, a therapeutic compound, producing DNA cross-links induced the highest level of DNA damages and EndoG expression. Incubation of CD4+ and CD8+ T-cells with cisplatin caused the changes in proportion of hTERT splice variants and inhibition of telomerase activity.

scholarly journals Isolation of a Candidate Human Telomerase Catalytic Subunit Gene, Which Reveals Complex Splicing Patterns in Different Cell Types

1997 ◽  
Vol 6 (12) ◽  
pp. 2011-2019 ◽  
Author(s):  
A. Kilian ◽  
D. D. L. Bowtell ◽  
H. E. Abud ◽  
G. R. Hime ◽  
D. J. Venter ◽  
...  
Keyword(s):  
Cell Types ◽  
Catalytic Subunit ◽  
Subunit Gene ◽  
Human Telomerase ◽  
Different Cell Types ◽  
Splicing Patterns
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