scholarly journals Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation

2016 ◽  
Vol 22 (40) ◽  
pp. 8918 ◽  
Author(s):  
Josieli Raskopf Colares ◽  
Elizângela Gonçalves Schemitt ◽  
Renata Minuzzo Hartmann ◽  
Francielli Licks ◽  
Mariana do Couto Soares ◽  
...  
Keyword(s):  
Bile Duct ◽  
Experimental Model ◽  
Bile Duct Ligation ◽  
Biliary Cirrhosis ◽  
Duct Ligation ◽  
Anti Inflammatory ◽  
Secondary Biliary Cirrhosis ◽  
Inflammatory Action

scholarly journals CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS

2015 ◽  
Vol 52 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Keli Cristina Simões da SILVEIRA ◽  
Cassiana Macagnan VIAU ◽  
Josiane Raskopf COLARES ◽  
Jenifer SAFFI ◽  
Norma Possa MARRONI ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Membrane Potential ◽  
Bile Duct ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Bile Duct Ligation ◽  
Decompensated Cirrhosis ◽  
Biliary Cirrhosis ◽  
Duct Ligation

Background Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to distinguish apoptotic from necrotic cells and comet assay was used for determined DNA integrity in single cells. Results In bile duct ligation animals there was significant increase in the kidney lipoperoxidation and an increase of the level of intracellular ROS. There was too an increase in the activity of all antioxidant enzymes evaluated in the kidney. The percentage viability was above 90% in the control group and in bile duct ligation was 64.66% and the dominant cell death type was apoptosis. DNA damage was observed in the bile duct ligation. There was a decreased in the mitochondrial membrane potential from 71.40% ± 6.35% to 34.48% ± 11.40% in bile duct ligation. Conclusions These results indicate that intracellular increase of ROS cause damage in the DNA and apoptosis getting worse the renal function in cirrhosis.

Chondroitin sulfate protects liver in the experimental model of extra-hepatic cholestasis induced by common bile duct ligation

2017 ◽  
Vol 66 (1) ◽  
pp. S389
Author(s):  
P.L.R. Guedes ◽  
M.L. Gazarini ◽  
M.Y. Icimoto ◽  
J.A. Aguiar ◽  
M. Kouyoumdjian ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Chondroitin Sulfate ◽  
Experimental Model ◽  
Bile Duct Ligation ◽  
Common Bile Duct Ligation ◽  
Duct Ligation

Differential alteration of cytochrome P450 isoenzymes in two experimental models of cirrhosis

2000 ◽  
Vol 78 (11) ◽  
pp. 912-919 ◽  
Author(s):  
Marie-Claude Bastien ◽  
François Leblond ◽  
Vincent Pichette ◽  
Jean-Pierre Villeneuve
Keyword(s):  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Bile Duct ◽  
Serum Albumin ◽  
Breath Test ◽  
Bile Duct Ligation ◽  
Biliary Cirrhosis ◽  
Breath Tests ◽  
Duct Ligation

Liver diseases are associated with a decrease in hepatic drug elimination, but there is evidence that cirrhosis does not result in uniform changes of cytochrome P450 (CYP) isoenzymes. The objective of this study was to determine the content and activity of four CYP isoenzymes in the bile duct ligation and carbon tetrachloride (CCl4)-induced models of cirrhosis. The hepatic content of CYP1A, CYP2C, CYP2E1, and CYP3A was measured by Western blot analysis. CYP activity in vivo was evaluated with breath tests using substrates specific for different isoenzymes: caffeine (CYP1A2), aminopyrine (CYP2C11), nitrosodimethylamine (CYP2E1), and erythromycin (CYP3A). Bile duct ligation resulted in biliary cirrhosis; CYP1A, CYP2C and CYP3A content was decreased and the caffeine, aminopyrine, and erythromycin breath tests were reduced whereas CYP2E1 content and the nitrosodimethylamine breath test were unchanged compared with controls. CCl4 treatment resulted in cirrhosis of varying severity as assessed from the decrease in liver weight and serum albumin. In rats with mild cirrhosis, CYP content was comparable with controls except for a decrease in CYP2C. The activity of CYPs was also unchanged except for an increase in CYP2E1 activity. In rats with more severe cirrhosis, the content of all four CYP isoenzymes and the caffeine, aminopyrine, and erythromycin breath tests were reduced whereas the nitrosodimethylamine breath test was unchanged. In both models of cirrhosis, there was a significant correlation between the breath tests results and the severity of cirrhosis as assessed from serum albumin levels. These results indicate that content and the catalytic activity of individual CYP enzymes are differentially altered by cirrhosis in the rat and also suggest that drug probes could be useful to assess hepatic functional reserve.Key words: breath test, cirrhosis, cytochrome P450, bile duct ligation, carbon tetrachloride.

scholarly journals Investigating the neuroprotective effects of Resveratrol on encephalopathy induced by bile duct ligation in male rats

2020 ◽  
Vol 10 (3) ◽  
pp. 5512-5515
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Male Rats ◽  
Definitive Treatment ◽  
Neuroprotective Effects ◽  
Beneficial Effects ◽  
Duct Ligation ◽  
Ischemic Diseases ◽  
Anti Inflammatory ◽  
First Time

Hepatic encephalopathy (HE) is a complex neuropsychiatric disorder without definitive treatment. The precise mechanism that leads to HE is not fully understood. Resveratrol (RES) is a polyphenol compound with antioxidant and anti-inflammatory properties that mitigates the progression of different illnesses such as inflammatory and ischemic diseases. This study reports the effects of RES on neuronal injures in bile duct-ligated rats. The rats were randomly distributed into three groups including sham, bile duct ligation (BDL), and BDL + RES. Behavioral and biochemical studies were performed to evaluate neuronal injuries. The obtained data indicate that BDL experienced a balanced impairment and an increase in the hepatic enzymes. RES had a preserving role in the treated animals. Moreover, RES treatment declined neuronal injuries induced by BDL. For the first time, the results of this study showed that RES has beneficial effects in the rat model of HE probably because of its antioxidant and anti-inflammatory properties.

scholarly journals Quercetin Treatment Ameliorates Systemic Oxidative Stress in Cirrhotic Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Emanuelle Kerber Vieira ◽  
Silvia Bona ◽  
Fábio Cangeri Di Naso ◽  
Marilene Porawski ◽  
Juliana Tieppo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bile Duct ◽  
Thiobarbituric Acid ◽  
Biliary Cirrhosis ◽  
Thiobarbituric Acid Reactive Substances ◽  
Common Bile Duct Ligation ◽  
Systemic Oxidative Stress ◽  
Duct Ligation ◽  
Secondary Biliary Cirrhosis ◽  
Quercetin Treatment

Our aim was to investigate whether the antioxidant quercetin protects against liver injury and ameliorates the systemic oxidative stress in rats with common bile duct ligation. Secondary biliary cirrhosis was induced through 28 days of bile duct obstruction. Animals received quercetin (Q) after 14 days of obstruction. Groups of control (CO) and cirrhotic (CBDL) animals received a daily 50 mg/kg body weight i.p. injection of quercetin (CO + Q; CBDL + Q) or vehicle (CO; CBDL). Quercetin corrected the reduction in superoxide dismutase (SOD), catalase CAT, and glutathione peroxidase GPx activities and prevented the increase of thiobarbituric acid reactive substances (TBARS), aminotransferases, and alkaline phosphatase in cirrhotic animals. Quercetin administration also corrected the reduced total nitrate concentration in the liver and prevented liver fibrosis and necrosis. These effects suggest that quercetin might be a useful agent to preserve liver function and prevent systemic oxidative stress.

Bile duct ligation in neonatal rats: Is it a valid experimental model for biliary atresia studies?

2009 ◽  
Vol 13 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Nelson Elias Mendes Gibelli ◽  
Uenis Tannuri ◽  
Evandro Sobroza de Mello ◽  
Consuelo Junqueira Rodrigues
Keyword(s):  
Bile Duct ◽  
Biliary Atresia ◽  
Experimental Model ◽  
Bile Duct Ligation ◽  
Neonatal Rats ◽  
Duct Ligation

Glutathione status in liver and plasma during development of biliary cirrhosis after bile duct ligation

1998 ◽  
Vol 198 (4) ◽  
pp. 167-174 ◽  
Author(s):  
Edmund Purucker ◽  
Ron Winograd ◽  
Elke Roeb ◽  
Siegfried Matern
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Biliary Cirrhosis ◽  
Glutathione Status ◽  
Duct Ligation

Effects of chronic L-NAME on nitrotyrosine expression and renal vascular reactivity in rats with chronic bile-duct ligation

2008 ◽  
Vol 115 (2) ◽  
pp. 57-68 ◽  
Author(s):  
Antonia Alcaraz ◽  
David Hernández ◽  
David Iyú ◽  
Rubén Mota ◽  
Noemí M. Atucha ◽  
...  
Keyword(s):  
Bile Duct ◽  
Vascular Reactivity ◽  
Bile Duct Ligation ◽  
Sodium Balance ◽  
Biliary Cirrhosis ◽  
Sodium Retention ◽  
Vascular Response ◽  
Experimental Cirrhosis ◽  
Duct Ligation ◽  
Renal Vascular

In liver cirrhosis, elevated levels of NO and ROS (reactive oxygen species) might greatly favour the generation of peroxynitrite. Peroxynitrite is a highly reactive oxidant and it can potentially alter the vascular reactivity and the function of different organs. In the present study, we evaluated whether peroxynitrite levels are related to the progression of renal vascular and excretory dysfunction during experimental cirrhosis induced by chronic BDL (bile-duct ligation) in rats. Experiments were performed at 7, 15 and 21 days after BDL in rats and in rats 21 days post-BDL chronically treated with L-NAME (NG-nitro-L-arginine methyl ester). Sodium balance, BP (blood pressure), basal RPP (renal perfusion pressure) and the renal vascular response to PHE (phenylephrine) and ACh (acetylcholine) in isolated perfused kidneys were measured. NO levels were calculated as 24-h urinary excretion of nitrites, ROS as TBARS (thiobarbituric acid-reacting substances), and peroxynitrite formation as the renal expression of nitrotyrosine. BDL rats had progressive sodium retention, and decreased BP, RPP and renal vascular responses to PHE and ACh in the time following BDL. They also had increasing levels of NO and ROS, and renal nitrotyrosine accumulation, especially in the medulla. All of these changes were either prevented or significantly decreased by chronic L-NAME administration. In conclusion, these results suggest that the increasing levels of peroxynitrite might contribute to the altered renal vascular response and sodium retention in the development of the experimental biliary cirrhosis. Moreover, the beneficial effects of decreasing NO synthesis are, at least in part, mediated by anti-peroxinitrite-related effects.

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