scholarly journals Quercetin Treatment Ameliorates Systemic Oxidative Stress in Cirrhotic Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Emanuelle Kerber Vieira ◽  
Silvia Bona ◽  
Fábio Cangeri Di Naso ◽  
Marilene Porawski ◽  
Juliana Tieppo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bile Duct ◽  
Thiobarbituric Acid ◽  
Biliary Cirrhosis ◽  
Thiobarbituric Acid Reactive Substances ◽  
Common Bile Duct Ligation ◽  
Systemic Oxidative Stress ◽  
Duct Ligation ◽  
Secondary Biliary Cirrhosis ◽  
Quercetin Treatment

Our aim was to investigate whether the antioxidant quercetin protects against liver injury and ameliorates the systemic oxidative stress in rats with common bile duct ligation. Secondary biliary cirrhosis was induced through 28 days of bile duct obstruction. Animals received quercetin (Q) after 14 days of obstruction. Groups of control (CO) and cirrhotic (CBDL) animals received a daily 50 mg/kg body weight i.p. injection of quercetin (CO + Q; CBDL + Q) or vehicle (CO; CBDL). Quercetin corrected the reduction in superoxide dismutase (SOD), catalase CAT, and glutathione peroxidase GPx activities and prevented the increase of thiobarbituric acid reactive substances (TBARS), aminotransferases, and alkaline phosphatase in cirrhotic animals. Quercetin administration also corrected the reduced total nitrate concentration in the liver and prevented liver fibrosis and necrosis. These effects suggest that quercetin might be a useful agent to preserve liver function and prevent systemic oxidative stress.

scholarly journals CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS

2015 ◽  
Vol 52 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Keli Cristina Simões da SILVEIRA ◽  
Cassiana Macagnan VIAU ◽  
Josiane Raskopf COLARES ◽  
Jenifer SAFFI ◽  
Norma Possa MARRONI ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Membrane Potential ◽  
Bile Duct ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Bile Duct Ligation ◽  
Decompensated Cirrhosis ◽  
Biliary Cirrhosis ◽  
Duct Ligation

Background Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to distinguish apoptotic from necrotic cells and comet assay was used for determined DNA integrity in single cells. Results In bile duct ligation animals there was significant increase in the kidney lipoperoxidation and an increase of the level of intracellular ROS. There was too an increase in the activity of all antioxidant enzymes evaluated in the kidney. The percentage viability was above 90% in the control group and in bile duct ligation was 64.66% and the dominant cell death type was apoptosis. DNA damage was observed in the bile duct ligation. There was a decreased in the mitochondrial membrane potential from 71.40% ± 6.35% to 34.48% ± 11.40% in bile duct ligation. Conclusions These results indicate that intracellular increase of ROS cause damage in the DNA and apoptosis getting worse the renal function in cirrhosis.

scholarly journals Antioxidant and anti-inflammatory action of melatonin in an experimental model of secondary biliary cirrhosis induced by bile duct ligation

2016 ◽  
Vol 22 (40) ◽  
pp. 8918 ◽  
Author(s):  
Josieli Raskopf Colares ◽  
Elizângela Gonçalves Schemitt ◽  
Renata Minuzzo Hartmann ◽  
Francielli Licks ◽  
Mariana do Couto Soares ◽  
...  
Keyword(s):  
Bile Duct ◽  
Experimental Model ◽  
Bile Duct Ligation ◽  
Biliary Cirrhosis ◽  
Duct Ligation ◽  
Anti Inflammatory ◽  
Secondary Biliary Cirrhosis ◽  
Inflammatory Action

Secondary biliary cirrhosis in the rat is prevented by decreasing NF-κ B nuclear translocation and TGF-β expression using allopurinol, an inhibitor of xanthine oxidase

2012 ◽  
Vol 90 (11) ◽  
pp. 1469-1478 ◽  
Author(s):  
Liseth R. Aldaba-Muruato ◽  
Mario G. Moreno ◽  
Elisa Hernández-Mercado ◽  
Mineko Shibayama ◽  
Pablo Muriel
Keyword(s):  
Oxidative Stress ◽  
Xanthine Oxidase ◽  
Transforming Growth Factor ◽  
Nuclear Translocation ◽  
Active Form ◽  
Transforming Growth Factor Β ◽  
Biliary Cirrhosis ◽  
Common Bile Duct Ligation ◽  
Experimental Cirrhosis ◽  
Duct Ligation

Allopurinol is an inhibitor of xanthine oxidase (XO), and XO is an enzyme that generates great amounts of reactive oxygen species. The aim of this work was to evaluate the efficacy of allopurinol to prevent experimental cirrhosis. Fibrosis and cirrhosis were induced by common bile duct ligation (BDL) for 4 weeks in rats. Animals were divided into 4 groups: sham-operated rats (SHAM); BDL group; BDL plus allopurinol (100 mg·kg–1, p.o.), and SHAM plus allopurinol treatment. Alanine aminotransferase, γ-glutamyl transpeptidase, and alkaline phosphatase were increased in BDL rats but were preserved normal by allopurinol. XO activity was prevented by allopurinol; however, lipophilic and hydrophilic oxidative stress was not prevented by the drug. Allopurinol partially suppresses nuclear factor-κB (NF-κB) nuclear translocation and transforming growth factor-β (TGF-β) expression, and increased the active form of matrix metalloproteinase-13 (MMP-13). Moreover, collagen production induced by BDL was partially but significantly reduced by allopurinol. These findings suggest that allopurinol possesses a hepatoprotective effect probably by modulating proteins such as NF-κB, TGF-β, and MMP-13, helping to protect against liver damage induced by chronic cholestasis and a mechanism independent of oxidative stress.

scholarly journals Melatonin Ameliorates Bile Duct Ligation-Induced Systemic Oxidative Stress and Spatial Memory Deficits in Developing Rats

2009 ◽  
Vol 65 (2) ◽  
pp. 176-180 ◽  
Author(s):  
Li-Tung Huang ◽  
Mao-Meng Tiao ◽  
You-Lin Tain ◽  
Chih-Cheng Chen ◽  
Chih-Sung Hsieh
Keyword(s):  
Oxidative Stress ◽  
Bile Duct ◽  
Spatial Memory ◽  
Bile Duct Ligation ◽  
Memory Deficits ◽  
Systemic Oxidative Stress ◽  
Duct Ligation

scholarly journals Study of the relation between biliary cirrhosis and hepatopulmonary syndrome and its reversibility among young rats submitted to common bile duct ligation

2018 ◽  
Vol 97 (3) ◽  
pp. 376-377
Author(s):  
Leonardo Ervolino Corbi ◽  
Maria Julia De Aro Braz ◽  
Ana Cristina Aoun Tannuri ◽  
Maria Cecília De Mendonça Coelho ◽  
Suellen Serafini ◽  
...  
Keyword(s):  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Duct Ligation ◽  
Hepatopulmonary Syndrome ◽  
Biliary Cirrhosis ◽  
Common Bile Duct Ligation ◽  
Young Rats ◽  
Duct Ligation

The various impacts of the hepatitis C virus core protein upon hepatic oxidative stress after common bile duct ligation and partial hepatectomy

Redox Report ◽  
2008 ◽  
Vol 13 (4) ◽  
pp. 172-178 ◽  
Author(s):  
Ming-Yu Chang ◽  
Jeng-Chang Chen ◽  
Chau-Ting Yeh ◽  
Cheng-Tang Chiu ◽  
Deng-Yn Lin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Bile Duct ◽  
Common Bile Duct ◽  
Bile Duct Ligation ◽  
Core Protein ◽  
Common Bile Duct Ligation ◽  
Virus Core ◽  
Duct Ligation
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