scholarly journals Comparison of Colonic Anastomosis Using Dry Amnion Membrane and Fibrin Glue in Intraperitoneal Infection Condition Assessed from Tissue Hydroxyproline Level Measurement (Study on Wistar Rat)

2021 ◽  
Keyword(s):  
Fibrin Glue ◽  
Colonic Anastomosis ◽  
Wistar Rat ◽  
Measurement Study ◽  
Level Measurement ◽  
Intraperitoneal Infection ◽  
Amnion Membrane

scholarly journals Comparison of metamizole and paracetamol effects on colonic anastomosis and fibroblast activities in Wistar rats

2020 ◽  
Author(s):  
Eko Purnomo ◽  
Dwi Aris Agung Nugrahaningsih ◽  
Nunik Agustriani ◽  
. Gunadi
Keyword(s):  
Granulation Tissue ◽  
Wistar Rats ◽  
Collagen Synthesis ◽  
Colonic Anastomosis ◽  
Wistar Rat ◽  
High Morbidity ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background : Leakage following colorectal anastomosis surgery causes various complications associated with high morbidity and mortality, especially in pediatric patients. It might be caused by the use of non-steroidal anti-inflammatory drugs (NSAIDs) as postoperative analgesics. This study aimed to compare the effect of metamizole and paracetamol on colonic anastomosis and fibroblast activities, including proliferation, migration, and collagen synthesis, in Wistar rats. Methods: Rats were divided into control, paracetamol and metamizole groups. The colonic anastomosis was evaluated by determining the integrity of the muscle layers, the formation of granulation tissue, and mucosal anastomosis. Fibroblast activities were analyzed by measuring the proliferation, migration, and collagen synthesis. Results: Metamizole caused more damage to muscle layer integrity, more inhibition of granulation tissue formation in the anastomosis area and lower mucosal anastomosis compared with paracetamol and control groups. Metamizole had a higher cytotoxic effect than paracetamol, which suppressed the proliferation and migration of fibroblasts. Furthermore, both drugs did not affect the synthesis of collagen. Conclusion: Metamizole shows worse effects on the integrity of muscle layers, inhibition of granulation tissue formation, mucosal anastomosis, fibroblast proliferation, and migration, but not collagen synthesis, than paracetamol in Wistar rat intestines following colonic anastomosis. These findings might indicate that paracetamol is safer than metamizole as analgesic following colonic anastomosis. Key Words: colonic anastomosis; fibroblast activities; metamizole; paracetamol; Wistar rat

scholarly journals Comparison of metamizole and paracetamol effects on colonic anastomosis and fibroblast activities in Wistar rats

2019 ◽  
Author(s):  
Eko Purnomo ◽  
Dwi Aris Agung Nugrahaningsih ◽  
Nunik Agustriani ◽  
. Gunadi
Keyword(s):  
Granulation Tissue ◽  
Wistar Rats ◽  
Collagen Synthesis ◽  
Colonic Anastomosis ◽  
Wistar Rat ◽  
High Morbidity ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background: Leakage following colorectal anastomosis surgery causes various complications associated with high morbidity and mortality, especially in pediatric patients. It might be caused by the use of non-steroidal anti-inflammatory drugs (NSAIDs) as postoperative analgesics. We aimed to compare the effect of metamizole and paracetamol on colonic anastomosis and fibroblast activities, including proliferation, migration, and collagen synthesis, in Wistar rats. Methods: We divided rats into control, paracetamol and metamizole groups. Colonic anastomosis was evaluated by determining the integrity of the muscle layers, formation of granulation tissue, and mucosal anastomosis. Fibroblast activities were analyzed by measuring the proliferation, migration, and collagen synthesis. Results: Metamizole caused more damage to muscle layer integrity, more inhibition of granulation tissue formation in the anastomosis area and lower mucosal anastomosis compared with paracetamol and control groups. Metamizole has a higher cytotoxic effect than paracetamol, which suppress the proliferation and migration of fibroblasts. Furthermore, both drugs did not affect the synthesis of collagen. Conclusion: Metamizole shows worse effects on integrity of muscle layers, inhibition of granulation tissue formation, mucosal anastomosis, fibroblast proliferation and migration, but not collagen synthesis, than paracetamol in Wistar rat intestines following colonic anastomosis. These findings might indicate that paracetamol is safer than metamizole as analgesic following colonic anastomosis. Keywords : colonic anastomosis; metamizole; paracetamol; fibroblast activities; Wistar rat

scholarly journals Comparison of metamizole and paracetamol effects on colonic anastomosis and fibroblast activities in Wistar rats

2019 ◽  
Author(s):  
Eko Purnomo ◽  
Dwi Aris Agung Nugrahaningsih ◽  
Nunik Agustriani ◽  
. Gunadi
Keyword(s):  
Granulation Tissue ◽  
Wistar Rats ◽  
Collagen Synthesis ◽  
Colonic Anastomosis ◽  
Wistar Rat ◽  
High Morbidity ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background : Leakage following colorectal anastomosis surgery causes various complications associated with high morbidity and mortality, especially in pediatric patients. It might be caused by the use of non-steroidal anti-inflammatory drugs (NSAIDs) as postoperative analgesics. This study aimed to compare the effect of metamizole and paracetamol on colonic anastomosis and fibroblast activities, including proliferation, migration, and collagen synthesis, in Wistar rats. Methods: Rats were divided into control, paracetamol and metamizole groups. The colonic anastomosis was evaluated by determining the integrity of the muscle layers, the formation of granulation tissue, and mucosal anastomosis. Fibroblast activities were analyzed by measuring the proliferation, migration, and collagen synthesis. Results: Metamizole caused more damage to muscle layer integrity, more inhibition of granulation tissue formation in the anastomosis area and lower mucosal anastomosis compared with paracetamol and control groups. Metamizole had a higher cytotoxic effect than paracetamol, which suppressed the proliferation and migration of fibroblasts. Furthermore, both drugs did not affect the synthesis of collagen. Conclusion: Metamizole shows worse effects on the integrity of muscle layers, inhibition of granulation tissue formation, mucosal anastomosis, fibroblast proliferation, and migration, but not collagen synthesis, than paracetamol in Wistar rat intestines following colonic anastomosis. These findings might indicate that paracetamol is safer than metamizole as analgesic following colonic anastomosis. Key Words: colonic anastomosis; fibroblast activities; metamizole; paracetamol; Wistar rat

Effects of the use of fibrin glue around the colonic anastomosis of the rat

2003 ◽  
Vol 7 (2) ◽  
pp. 82-84 ◽  
Author(s):  
I. Kanellos ◽  
I. Mantzoros ◽  
I. Goulimaris ◽  
E. Zacharakis ◽  
A. Zavitsanakis ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Colonic Anastomosis

scholarly journals The Effect of Fibrin Glue on the Intensity of Colonic Anastomosis in the Presence and Absence of Peritonitis: An Experimental Randomized Controlled Trial on Rats

ISRN Surgery ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Metin Senol ◽  
Mehmet M. Altintas ◽  
Ayhan Cevık ◽  
Yunus E. Altuntas ◽  
Nagehan O. Barisik ◽  
...  
Keyword(s):  
Fibrin Glue ◽  
Colonic Anastomosis ◽  
Controlled Trial ◽  
Proximal Colon ◽  
Control Group ◽  
Colon Anastomosis ◽  
Randomized Controlled ◽  
Bursting Pressures ◽  
The Impact ◽  
Experimental Group

Aim. Anastomotic leakage after colon anastomosis is the most frequent and most feared complication with its highest mortality rate. In this study, we aimed to expose the impact of performing fibrin glue on sutured colocolic anastomosis, in the presence of experimental peritonitis, on anastomosis safety. Method. In this experimental study, the rats were divided into two groups as control group (Groups 1 and 3) and experimental group (Groups 2 and 4). They were also divided as clean abdomen (Groups 1 and 2) and infected abdomen (3 and 4) groups. Full-thickness incisions were made on the proximal colon of both groups of rats. The control group’s anastomoses were conducted only with sutures, whereas in experimental group, fibrin glue was applied over the sutures. The samples were taken on the 10th day. Results. Highest values for average levels of hydroxyproline in the tissues and anastomotic bursting pressures were detected when fibrin glue was applied on sutured anastomosis in clean abdomen. In the histopathological staging performed in line with Ehrlich-Hunt model, lowest values were detected during the presence of peritonitis. Conclusion. As a result, it has been established that the use of fibrin glue over sutured colocolic anastomosis, both in clean abdomen and in the presence of peritonitis, had increased anastomosis safety.

Surface epithelial ultrastructure of the polycystic ovary in the BB wistar rat

1986 ◽  
Vol 44 ◽  
pp. 252-253
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Wistar Rat ◽  
Polycystic Ovaries ◽  
Chemical Diabetes ◽  
Scanning Electron

Diabetes decreases the fertility of females, but the mechanisms are not completely understood. In our investigations, we have found that 13% of the female BB Wistar rats that spontaneously developed chemical diabetes had persistent estrous. In this study the ovaries of these rats were examined by scanning electron microscopy(SEM) and compared to normal-cycling controls as well as to rats that had developed polycystic ovaries(PCO) by exposure to constant 1ight.

Ultrastructural and immunocytochemical studies of the rat hypothalamo-neurohypophysial system processed by rapid freezing and freeze-substitution fixation

1990 ◽  
Vol 48 (3) ◽  
pp. 884-885
Author(s):  
Seiji Shioda ◽  
Yasumitsu Nakai ◽  
Atsushi Ichikawa ◽  
Hidehiko Ochiai ◽  
Nobuko Naito
Keyword(s):  
Osmium Tetroxide ◽  
Freeze Substitution ◽  
Ultrastructural Localization ◽  
Wistar Rat ◽  
Neurosecretory Cells ◽  
Rapid Freezing ◽  
Sodium Metaperiodate ◽  
Tissue Sections ◽  
Ultrathin Sections ◽  
Electron Microscopes

The ultrastructure of neurosecretory cells and glia cells in the supraoptic nucleus (SON) of the hypothalamus and the neurohypophysis (PN) was studied after rapid freezing followed by substituion fixation. Also, the ultrastructural localization of vasopressin (VP) or its carrier protein neurophys in II (NPII) in the SON and PN was demonstrated by using a post-embedding immunoco1loidal gold staining method on the tissue sections processed by rapid freezing and freeze-substitution fixation.Adult male Wistar rat hypothalamus and pituitary gland were quenched by smashing against a copper block surface precooled with liquid helium and freeze-substituted in 3% osmium tetroxide-acetone solutions kept at -80°C for 36-48h. After substituion fixation, the tissue blocks were warmed up to room temperature, washed in acetone and then embedded in an Epon-Araldite mixture. Ultrathin sections mounted on 200 mesh nickel grids were immersed in saturated sodium metaperiodate and then incubated in each of the following solutions: 1 % egg albumin in phosphate buffer, VP or NPII (1/1000-1/5000) antiserum 24h at 4°C, 3) colloidal gold solution (1/20) 1h at 20°C. The sections were washed with distilled waterand dried, then stained with uranylacetate and lead citrate and examined with Hitachi HU-12A and H-800 electron microscopes.

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