In vitro Antiviral Activity of Bacopa monnieri (l.) Wettst Roots Methanolic Extract Against Dengue Virus Type 2

Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.

Download Full-text

In-vitro effect of human cathelicidin antimicrobial peptide LL-37 on dengue virus type 2

Peptides ◽

10.1016/j.peptides.2017.04.002 ◽

2017 ◽

Vol 92 ◽

pp. 23-30 ◽

Cited By ~ 25

Author(s):

K. Alagarasu ◽

P.S. Patil ◽

P. Shil ◽

M. Seervi ◽

M.B. Kakade ◽

...

Keyword(s):

Dengue Virus ◽

Antimicrobial Peptide ◽

Virus Type ◽

Vitro Effect ◽

Dengue Virus Type 2

Download Full-text

Antiviral activity of Faramea bahiensis leaves on dengue virus type-2 and characterization of a new antiviral flavanone glycoside

Phytochemistry Letters ◽

10.1016/j.phytol.2017.01.013 ◽

2017 ◽

Vol 19 ◽

pp. 220-225 ◽

Cited By ~ 6

Author(s):

Adriana C. Nascimento ◽

Ligia M.M. Valente ◽

Mário Gomes ◽

Rodolfo S. Barboza ◽

Thiago Wolff ◽

...

Keyword(s):

Antiviral Activity ◽

Dengue Virus ◽

Virus Type ◽

Flavanone Glycoside ◽

Dengue Virus Type 2

Download Full-text

Characterization of 8-hydroxyquinoline derivatives containing aminobenzothiazole as inhibitors of dengue virus type 2 protease in vitro

Antiviral Research ◽

10.1016/j.antiviral.2012.10.009 ◽

2013 ◽

Vol 97 (1) ◽

pp. 74-80 ◽

Cited By ~ 29

Author(s):

Huiguo Lai ◽

G. Sridhar Prasad ◽

Radhakrishnan Padmanabhan

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Dengue Virus Type 2

Download Full-text

Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation

Journal of General Virology ◽

10.1099/vir.0.81958-0 ◽

2006 ◽

Vol 87 (8) ◽

pp. 2263-2268 ◽

Cited By ~ 26

Author(s):

Dennis J. Pierro ◽

Ma Isabel Salazar ◽

Barry J. Beaty ◽

Ken E. Olson

Keyword(s):

Cell Culture ◽

Dengue Virus ◽

Virus Type ◽

Mammalian Cells ◽

Infectious Clone ◽

Cell Types ◽

Dengue Virus Type 2

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

Download Full-text

Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

The Scientific World JOURNAL ◽

10.1155/2013/282734 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 36

Author(s):

Kleber Juvenal Silva Farias ◽

Paula Renata Lima Machado ◽

Benedito Antônio Lopes da Fonseca

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Mammalian Cells ◽

Plaque Assay ◽

Antiviral Drug ◽

Vero Cells ◽

Significant Difference ◽

Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

Download Full-text