scholarly journals Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation

2006 ◽  
Vol 87 (8) ◽  
pp. 2263-2268 ◽  
Author(s):  
Dennis J. Pierro ◽  
Ma Isabel Salazar ◽  
Barry J. Beaty ◽  
Ken E. Olson
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Infectious Clone ◽  
Cell Types ◽  
Dengue Virus Type 2

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

scholarly journals Chloroquine Inhibits Dengue Virus Type 2 Replication in Vero Cells but Not in C6/36 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Kleber Juvenal Silva Farias ◽  
Paula Renata Lima Machado ◽  
Benedito Antônio Lopes da Fonseca
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Plaque Assay ◽  
Antiviral Drug ◽  
Vero Cells ◽  
Significant Difference ◽  
Dengue Virus Type 2

Dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. Since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. Chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal pH, was used in the in vitro treatment of Vero and C6/36 cells infected with dengue virus type 2 (DENV-2). Real-time RT-PCR and plaque assays were used to quantify the DENV-2 load in infected Vero and C6/36 cells after chloroquine treatment. Our results showed that a dose of 50 μg/ml of chloroquine was not toxic to the cells and induced a statistically significant inhibition of virus production in infected Vero cells when compared to untreated cells. In C6/36 cells, chloroquine does not induce a statistically significant difference in viral replication when compared to untreated cells, showing that this virus uses an unlikely pathway of penetration in these cells, and results were also confirmed by the plaque assay (PFU). These data suggest that the inhibition of virus infection induced by chloroquine is due to interference with acidic vesicles in mammalian cells.

In-vitro effect of human cathelicidin antimicrobial peptide LL-37 on dengue virus type 2

Peptides ◽  
2017 ◽  
Vol 92 ◽  
pp. 23-30 ◽  
Author(s):  
K. Alagarasu ◽  
P.S. Patil ◽  
P. Shil ◽  
M. Seervi ◽  
M.B. Kakade ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Antimicrobial Peptide ◽  
Virus Type ◽  
Vitro Effect ◽  
Dengue Virus Type 2

Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2

2006 ◽  
Vol 139 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Chie Aoki ◽  
Kazuya I.P.J. Hidari ◽  
Saki Itonori ◽  
Akihiro Yamada ◽  
Naonori Takahashi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Identification And Characterization ◽  
Dengue Virus Type 2

scholarly journals A small molecule inhibitor of dengue virus type 2 protease inhibits the replication of all four dengue virus serotypes in cell culture

2015 ◽  
Vol 12 (1) ◽  
pp. 16 ◽  
Author(s):  
Rajendra Raut ◽  
Hemalatha Beesetti ◽  
Poornima Tyagi ◽  
Ira Khanna ◽  
Swatantra K Jain ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Small Molecule ◽  
Small Molecule Inhibitor ◽  
Molecule Inhibitor ◽  
Dengue Virus Serotypes ◽  
Dengue Virus Type 2

scholarly journals Replication of Dengue Virus Type 2 in Aedes albopictus Cell Culture

1973 ◽  
Vol 12 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Pantipa Sinarachatanant ◽  
Lloyd C. Olson
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Albopictus Cell ◽  
Dengue Virus Type 2

Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro

2013 ◽  
Vol 159 (3) ◽  
pp. 573-579 ◽  
Author(s):  
Leonardo Padilla-S ◽  
Andrés Rodríguez ◽  
María M. Gonzales ◽  
Juan C. Gallego-G ◽  
Jhon C. Castaño-O
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Inhibitory Effects ◽  
Infected Cells ◽  
Dengue Virus Type 2
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