scholarly journals FATTY ACID AND AMINO ACID PROFILE OF INDIGENOUS CLUSTER ECOTYPES OF KENYAN FREERANGING CHICKEN

10.37512/100 ◽  
2019 ◽  

An experiment was conducted to determine amino acid and fatty acid profile of indigenous chicken reared in Kenya under a free range feeding system. Five cocks of each ecotype were purchased, slaughtered and transported to JKUAT and Durban University of Technology, within 12 and 24 hours respectively in an insulated box containing ice. Amino acid profile was estimated using Pico Tag by hydrolysis with acid for 24 hours, derivatized and analyzed by reverse phase HPLC. Fatty Acid Methyl Esters (FAME) were synthesized by a direct/one-step extraction transesterification method. FAME were then separated and quantified using gas chromatography. The amino acid and fatty acid profile of indigenous chicken in Kenya was affected by the ecotype as well as the part of the chicken (breast or thigh). The amino acid profile of experimental chicken revealed values higher than the RDA. The ratio of ꞷ-6:ꞷ-3 though higher than 4:1, demonstrated that the consumption of indigenous free ranging chicken meat is likely to give favourable health outcomes.

2019 ◽  
Vol 64 (No. 3) ◽  
pp. 141-149 ◽  
Author(s):  
Tingting Zhang ◽  
Bingwen Si ◽  
Yan Tu ◽  
Kai Cui ◽  
Chaolong Zhou ◽  
...  

A total of 144 cross-bred (Duroc × Landrace × Yorkshire) finishing pigs with a similar initial weight (65.4 ± 1.03 kg) were used to investigate the effect of moringa leaf meal on growth performance, meat quality, fatty acid composition, and amino acid profile. The moringa leaf meal-inclusion rates were 0% (M0 or control), 3% (M3), 6% (M6) and 9% (M9). The results showed that supplementing moringa leaf meal significantly increased the daily weight gain of finishing pigs, but had a minor impact on pork quality indicators and the amino acid profile in the Longissimus dorsi. The fatty acid profile in the Longissimus dorsi was significantly modified when pigs were fed moringa leaf meal diets. The relative percentage of total unsaturated fatty acid and monounsaturated fatty acid was higher in the meat of pigs fed a 6% moringa leaf meal diet than in the meat of those receiving the control diet. In contrast, the percentage of total saturated fatty acids was lower in the meat of pigs fed the moringa leaf meal diets. Moreover, the omega-6/omega-3 ratio decreased with moringa leaf meal supplementation. Addition of the moringa supplement into the diet of pigs improved growth performance and modified pork fatty acid profile positively. The results suggest that moringa leaf meal could be used as a diet supplement for producing healthier pork.


2021 ◽  
pp. e00896
Author(s):  
Paul Taipa Leparmarai ◽  
Carmen Kunz ◽  
David Miano Mwangi ◽  
Ilona Gluecks ◽  
Michael Kreuzer ◽  
...  

2015 ◽  
Vol 80 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Jun Wang ◽  
Weiwei Wu ◽  
Xudong Wang ◽  
Min Wang ◽  
Fuan Wu

In search of an accurate and effective method to determine fatty acid composition in silkworm pupae oils, five methylation methods were evaluated for use in the gas chromatographic (GC) quantitation of fatty acid methyl esters (FAMEs), including one-step esterification catalyzed by an acidic (H2SO4 and BF3) or alkali catalyst (KOH and NaOCH3) and a two-step procedure catalyzed successively by KOH and H2SO4. These methods were comparatively adopted to quantify FAMEs in silkworm pupae oil using GC-MS and GC and then validate their precision, stability and average recovery rates. The results indicated that compared with the other four methyl esterification methods, two-step methylation effectively improves the synthesis yield of FAMEs, conserves agents, and eliminates the usage of potential harmful reagents. The proposed GC method has been validated, shows good accuracy and precision, and has been applied successfully to the quantification of FAMEs in several varieties of silkworm pupae oils. The short analytical run time leads to low costs and a fast chromatographic procedure. In summary, two-step pretreatment had superior performance, providing technical references for the determination and analysis of fatty acids in other oils.


2021 ◽  
Vol 51 (2) ◽  
pp. 262-270
Author(s):  
I.M. Boldea ◽  
C. Dragomir ◽  
M.A. Gras ◽  
M. Ropotă

The objective of this research was to assess the effects of including oil-rich feedstuffs in diets for lactating goats on the fatty acid (FA) profile of their milk. Thirty-six Murciano-Granadina goats were randomly assigned to three treatment groups, namely a control diet (CTRL), a diet based on whole rapeseed (RS), and a diet based on pumpkin seed cake (PSC). The diets were composed of 1 kg hay (70 % Italian ryegrass, 30% alfalfa) and 1.24 kg concentrate, and were formulated to be isoenergetic and isonitrogenous. Milk yield and its contents of protein, fat and lactose did not differ significantly among the groups. However, including oil-rich feeds in the diet altered the fatty acid profile of the milk significantly, decreasing its saturated fatty acid (SFA) content and increasing its content of unsaturated fatty acids (UFAs). Effects on polyunsaturated fatty acids (PUFAs), conjugated linoleic acid (CLA), and the n-6 to n-3 ratio depended on the source of dietary lipids. The PSC augmented diet increased the relative amount of PUFAs and fatty acid methyl esters (FAME) in milk (+25 %) significantly In comparison with CTRL, whereas the RS diet produced a limited and statistically insignificant increase (+7.5%). The concentration of CLA was higher in milk from does fed the PSC diet, whereas the n-6 to n-3 ratio was lower in milk from does fed RS. These preliminary results form the basis for developing premium dairy products that are enriched in fatty acids that are more favourable for human health.


1974 ◽  
Vol 31 (6) ◽  
pp. 1025-1029 ◽  
Author(s):  
John Spinelli ◽  
Lawrence Lehman ◽  
Dave Wieg

The proximate composition, amino acid profile, and fatty acid composition of red crab (Pleuroncodes planipes) indicated that it was highly suitable for inclusion in salmonid diets. The amino acid profile of the proteins was similar to those of fish and the fatty acid composition was approximately 40% linolenic. Rainbow trout fed Oregon Moist Pellet (OMP) type diets containing 10 and 25% red crab were highly pigmented after 2 mo.A method for processing red crab into a dry feed material and a chitinous residue suitable for further processing into chitin derivatives was described.


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