scholarly journals The nanofluidic confinement apparatus: studying confinement-dependent nanoparticle behavior and diffusion

2018 ◽  
Vol 9 ◽  
pp. 301-310 ◽  
Author(s):  
Stefan Fringes ◽  
Felix Holzner ◽  
Armin W Knoll

The behavior of nanoparticles under nanofluidic confinement depends strongly on their distance to the confining walls; however, a measurement in which the gap distance is varied is challenging. Here, we present a versatile setup for investigating the behavior of nanoparticles as a function of the gap distance, which is controlled to the nanometer. The setup is designed as an open system that operates with a small amount of dispersion of ≈20 μL, permits the use of coated and patterned samples and allows high-numerical-aperture microscopy access. Using the tool, we measure the vertical position (termed height) and the lateral diffusion of 60 nm, charged, Au nanospheres as a function of confinement between a glass surface and a polymer surface. Interferometric scattering detection provides an effective particle illumination time of less than 30 μs, which results in lateral and vertical position detection accuracy ≈10 nm for diffusing particles. We found the height of the particles to be consistently above that of the gap center, corresponding to a higher charge on the polymer substrate. In terms of diffusion, we found a strong monotonic decay of the diffusion constant with decreasing gap distance. This result cannot be explained by hydrodynamic effects, including the asymmetric vertical position of the particles in the gap. Instead we attribute it to an electroviscous effect. For strong confinement of less than 120 nm gap distance, we detect the onset of subdiffusion, which can be correlated to the motion of the particles along high-gap-distance paths.

2011 ◽  
Vol 39 (1) ◽  
pp. 109-118 ◽  
Author(s):  
Martin F. Fast ◽  
Andreas Krauss ◽  
Uwe Oelfke ◽  
Simeon Nill

Author(s):  
Ryoken MASUDA ◽  
Ryoga YUZAWA ◽  
Mitsuhide SATO ◽  
Tsutomu MIZUNO ◽  
Kunihisa TASHIRO ◽  
...  

Sensors ◽  
2020 ◽  
Vol 20 (11) ◽  
pp. 3115
Author(s):  
Wei Yang ◽  
Xiang Zhang ◽  
Qian Lei ◽  
Dengye Shen ◽  
Ping Xiao ◽  
...  

Accurate detection of lane lines is of great significance for improving vehicle driving safety. In our previous research, by improving the horizontal and vertical density of the detection grid in the YOLO v3 (You Only Look Once, the 3th version) model, the obtained lane line (LL) algorithm, YOLO v3 (S × 2S), has high accuracy. However, like the traditional LL detection algorithms, they do not use spatial information and have low detection accuracy under occlusion, deformation, worn, poor lighting, and other non-ideal environmental conditions. After studying the spatial information between LLs and learning the distribution law of LLs, an LL prediction model based on long short-term memory (LSTM) and recursive neural network (RcNN) was established; the method can predict the future LL position by using historical LL position information. Moreover, by combining the LL information predicted with YOLO v3 (S × 2S) detection results using Dempster Shafer (D-S) evidence theory, the LL detection accuracy can be improved effectively, and the uncertainty of this system be reduced correspondingly. The results show that the accuracy of LL detection can be significantly improved in rainy, snowy weather, and obstacle scenes.


1996 ◽  
Vol 16 (5) ◽  
pp. 391-404 ◽  
Author(s):  
Birgitta J. Rasmusson ◽  
Jean-Louis Carpentier ◽  
Jean-Pierre Paccaud ◽  
Karl-Eric Magnusson

The effects of the N-formyl methionyl peptide, formyl-methionyl-leucyl phenylalanine (fMLF) on the lateral mobility of the complement receptor type 1 (CR1/CD35) in glass-adherent human neutrophils were investigated, using fluorescence recovery after photobleaching (FRAP) and confocal microscopy (CSLM). It was found that addition of 0.1–1 μM fMLF increased the diffusion constant (D) of CR1/CD35 to 167–278% of controls. No effect was observed on the receptor distribution or the mobile fraction of receptors. The effect of fMLF on the lateral diffusion of CR1/CD35 could be totally inhibited by addition of pertussis toxin (PT, 250 ng/ml) or of the free radical scavenger enzymes superoxide dismutase (SOD, 2000 U/ml) and catalase (CAT, 200 U/ml), added together the results show that oxidative metabolites produced by neutrophils in response to fMLF can modulate CR1/CD35 diffusion, and indicate a regulatory role for oxygen radicals in phagocytosis.


2011 ◽  
Vol 38 (6Part28) ◽  
pp. 3757-3758
Author(s):  
M Fast ◽  
A Krauss ◽  
S Nill ◽  
U Oelfke

Sensors ◽  
2019 ◽  
Vol 19 (21) ◽  
pp. 4722 ◽  
Author(s):  
Zhang ◽  
Guo ◽  
Zhang ◽  
Zhao

For the laser tracking and positioning system of a moving target using a four-quadrant detector, the accuracy of laser spot position detection has a serious impact on the tracking performance of the system. For moving target tracking, the traditional spot position detection method of a four-quadrant detector cannot give better consideration to both detection accuracy and operation speed. In view of this, an improved method based on piecewise low-order polynomial least squares fitting and a Kalman filter is proposed. Firstly, the tracking and positioning mathematical model of the system is created, and the experimental device is established. Then, the shortcomings of traditional methods are analyzed, and the improved method and the real-time tracking and positioning algorithm of the system are studied. Finally, through the experiment, the system operation effects are compared and analyzed before and after the improvement. The experimental results of system dynamic tracking show that, the least squares fitting of the experimental data using a 5-segment and quadratic polynomial can achieve better results. By using the improved method, the maximum tracking distance of a moving object is increased from 12 m to more than 30 m. At a distance of 7.5 m, the maximum tracking speed can reach 2.11 m/s, and the root mean square error (RMSE) of the position is less than 4.59 mm. At 15.5 m, the maximum tracking speed is 2.04 m/s and the RMSE is less than 5.42 mm. Additionally, at 23.5 m, it is 1.13 m/s and 5.71 mm.


2016 ◽  
Vol 5 (1) ◽  
pp. 213-220 ◽  
Author(s):  
Sebastian Felix Reinecke ◽  
Uwe Hampel

Abstract. A concept for buoyancy control and magnetic position detection has been developed for the improvement of instrumented flow-following sensor particles. The sensor particles are used for investigation of hydrodynamic and biochemical processes in large-scale vessels such as biogas fermenters, bioreactors and aerated sludge basins. Neutral buoyancy of the sensor particles is required for tracing of the fluid flows. Buoyancy control is performed by adjustment of the sensor particles' volume, which is altered by an integrated piston. A miniaturized linear actuator, namely a stepper motor with linear transmission, is operated by a microcontroller to drive the piston. The buoyancy control unit enables accurate automated taring of the sensor particles in the stagnant process fluid to achieve neutral buoyancy. Therefore, the measured vertical position of the sensor particle as a function of the hydrostatic pressure is used as feedback. It has an incremental density change of 0.0136 % as compared to water and a residual drift velocity of approximately 3.6  ×  10−3 m s−1. Furthermore, a minimum density of 926 kg m−3 can be set by full extension of the piston, which allows floating of the sensor particles after a defined event, namely critical charge of battery, full data storage or the end of a fixed time cycle. Thus, recovery of the sensor particles can proceed easily from the fluid level. The sensor particles with a buoyancy control unit are tested for depths up to 15 m. Also, detection of a local magnetic position marker by the sensor particles has been implemented to enhance movement tracking. It was tested in a lab-scale biogas digester and was used for estimation of the liquid circulation time distribution and Peclét number to describe the macro-flow.


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