STUDY OF SACCHAROMYCES CEREVISIAE YEAST STRAIN SENSITIVITY WITH DOUBLE MUTANT RAD2∆HSM3∆ TO METHYLMETHANESULPHONATE

2020 ◽  
pp. 325-328
Author(s):  
E. Alekseeva ◽  
V. Korolev
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Strain ◽  
Double Mutant ◽  
Methyl Methanesulfonate ◽  
Strain Sensitivity ◽  
Spontaneous Mutagenesis

The sensitivity of the yeast strain Saccharomyces cerevisiae with the double mutant rad2∆hsm3∆ to genotoxicants was studied using methyl methanesulfonate as an example, as well as its influence on the possibility of spontaneous mutagenesis, and further use of the strain as a test for determining genotoxicants in the environment.

scholarly journals INCREASED SPONTANEOUS MITOTIC SEGREGATION IN MMS-SENSITIVE MUTANTS OF SACCHAROMYCES CEREVISIAE

Genetics ◽  
1977 ◽  
Vol 87 (2) ◽  
pp. 229-236
Author(s):  
Satya Prakash ◽  
Louise Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Methyl Methanesulfonate ◽  
X Rays ◽  
X Ray ◽  
Complementation Groups

ABSTRACT Methyl methanesulfonate (MMS)-sensitive mutants of Saccharomyces cerevisiae belonging to four different complementation groups, when homozygous, increase the rate of spontaneous mitotic segregation to canavanine resistance from heterozygous sensitive (canr/+) diploids by 13- to 170-fold. The mms8—1 mutant is MMS and X-ray sensitive and increases the rate of spontaneous mitotic segregation 170-fold. The mms9—1 and mms13—1 mutants are sensitive to X rays and UV, respectively, in addition to MMS, and increase the rate of spontaneous mitotic segregation by 13-fold and 85-fold, respectively. The mutant mms21—1 is sensitive to MMS, X rays and UV and increases the rate of spontaneous mitotic segregation 23-fold.

Evaluation of the roles of Pol zeta and NHEJ in starvation-associated spontaneous mutagenesis in the yeast Saccharomyces cerevisiae

2009 ◽  
Vol 55 (3) ◽  
pp. 245-251 ◽  
Author(s):  
Agnieszka Halas ◽  
Hanna Baranowska ◽  
Agnieszka Podlaska ◽  
Ewa Sledziewska-Gojska
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

scholarly journals A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Bernadette Connors ◽  
Lauren Rochelle ◽  
Asela Roberts ◽  
Graham Howard
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Excision Repair ◽  
Uv Irradiation ◽  
Double Mutant ◽  
Excision Repair ◽  
Strand Break ◽  
Anaphase Promoting Complex ◽  
End Joining ◽  
Ubiquitin Ligase Complex ◽  
Nucleotide Excision

Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER) and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C), a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair.

Mutation in genes coding for glucose-induced degradation-deficient protein contribute to high malate production in yeast strains No. 28 and No. 77 used for industrial brewing of sake

2021 ◽  
Author(s):  
Hiroaki Negoro ◽  
Atsushi Kotaka ◽  
Hiroki Ishida
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Organic Acids ◽  
Nonsense Mutation ◽  
Yeast Strain ◽  
Homozygous Mutation ◽  
Alcohol Fermentation ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Yeast Strains

ABSTRACT Saccharomyces cerevisiae produces organic acids including malate during alcohol fermentation. Since malate contributes to the pleasant flavor of sake, high-malate-producing yeast strain No. 28 and No. 77 have been developed by the Brewing Society of Japan. In this study, the genes responsible for the high malate phenotype in these strains were investigated. We had found previously that the deletion of components of the glucose induced degradation-deficient (GID) complex led to high malate production in yeast. Upon examining GID protein-coding genes in yeast strain No. 28 and No. 77, a nonsense homozygous mutation of GID4 in strain No. 28, and of GID2 in strain No. 77, were identified as the cause of high malate production. Furthermore, complementary tests of these mutations indicated that the heterozygous nonsense mutation in GID2 was recessive. In contrast, the heterozygous nonsense mutation in GID4 was considered semi-dominant.

DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

SPT10 and SPT21 are required for transcription of particular histone genes in Saccharomyces cerevisiae

1994 ◽  
Vol 14 (8) ◽  
pp. 5223-5228
Author(s):  
C Dollard ◽  
S L Ricupero-Hovasse ◽  
G Natsoulis ◽  
J D Boeke ◽  
F Winston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Differential Expression ◽  
Double Mutant ◽  
The Other ◽  
Histone Genes ◽  
Related Gene ◽  
Histone Proteins ◽  
Histone Locus

The Saccharomyces cerevisiae genome contains four loci that encode histone proteins. Two of these loci, HTA1-HTB1 and HTA2-HTB2, each encode histones H2A and H2B. The other two loci, HHT1-HHF1 and HHT2-HHF2, each encode histones H3 and H4. Because of their redundancy, deletion of any one histone locus does not cause lethality. Previous experiments demonstrated that mutations at one histone locus, HTA1-HTB1, do cause lethality when in conjunction with mutations in the SPT10 gene. SPT10 has been shown to be required for normal levels of transcription of several genes in S. cerevisiae. Motivated by this double-mutant lethality, we have now investigated the interactions of mutations in SPT10 and in a functionally related gene, SPT21, with mutations at each of the four histone loci. These experiments have demonstrated that both SPT10 and SPT21 are required for transcription at two particular histone loci, HTA2-HTB2 and HHF2-HHT2, but not at the other two histone loci. These results suggest that under some conditions, S. cerevisiae may control the level of histone proteins by differential expression of its histone genes.

Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28

2012 ◽  
Vol 114 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Shunichi Nakayama ◽  
Ken Tabata ◽  
Takahiro Oba ◽  
Kenichi Kusumoto ◽  
Shinji Mitsuiki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Yeast Strain ◽  
Acid Production ◽  
Production Mechanism

scholarly journals Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Qiaoning He ◽  
Yongfu Yang ◽  
Shihui Yang ◽  
Bryon S. Donohoe ◽  
Stefanie Van Wychen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Strain

scholarly journals New Lager Brewery Strains Obtained by Crossing Techniques Using Cachaça (Brazilian Spirit) Yeasts

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Bruna Inez Carvalho Figueiredo ◽  
Margarete Alice Fontes Saraiva ◽  
Paloma Patrick de Souza Pimenta ◽  
Miriam Conceição de Souza Testasicca ◽  
Geraldo Magela Santos Sampaio ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Temperature ◽  
Yeast Strain ◽  
Genetically Modified ◽  
Acetate Esters ◽  
Content Type ◽  
Yeast Strains ◽  
Lager Beer ◽  
New Yeast ◽  
Beer Production

ABSTRACT The development of hybrids has been an effective approach to generate novel yeast strains with optimal technological profile for use in beer production. This study describes the generation of a new yeast strain for lager beer production by direct mating between two Saccharomyces cerevisiae strains isolated from cachaça distilleries: one that was strongly flocculent, and the other with higher production of acetate esters. The first step in this procedure was to analyze the sporulation ability and reproductive cycle of strains belonging to a specific collection of yeasts isolated from cachaça fermentation vats. Most strains showed high rates of sporulation, spore viability, and homothallic behavior. In order to obtain new yeast strains with desirable properties useful for lager beer production, we compare haploid-to-haploid and diploid-to-diploid mating procedures. Moreover, an assessment of parental phenotype traits showed that the segregant diploid C2-1d generated from a diploid-to-diploid mating experiment showed good fermentation performance at low temperature, high flocculation capacity, and desirable production of acetate esters that was significantly better than that of one type lager strain. Therefore, strain C2-1d might be an important candidate for the production of lager beer, with distinct fruit traces and originating using a non-genetically modified organism (GMO) approach. IMPORTANCE Recent work has suggested the utilization of hybridization techniques for the generation of novel non-genetically modified brewing yeast strains with combined properties not commonly found in a unique yeast strain. We have observed remarkable traits, especially low temperature tolerance, maltotriose utilization, flocculation ability, and production of volatile aroma compounds, among a collection of Saccharomyces cerevisiae strains isolated from cachaça distilleries, which allow their utilization in the production of beer. The significance of our research is in the use of breeding/hybridization techniques to generate yeast strains that would be appropriate for producing new lager beers by exploring the capacity of cachaça yeast strains to flocculate and to ferment maltose at low temperature, with the concomitant production of flavoring compounds.

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