scholarly journals Orthotopic Mouse Model of Colorectal Cancer

10.3791/484 ◽  
2007 ◽  
Author(s):  
William Tseng ◽  
Xianne Leong ◽  
Edgar Engleman
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Orthotopic Mouse Model

scholarly journals Establishment of an Endoscopy-Guided Minimally Invasive Orthotopic Mouse Model of Colorectal Cancer

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 3007
Author(s):  
Chen Chen ◽  
Jens Neumann ◽  
Florian Kühn ◽  
Serene M. L. Lee ◽  
Moritz Drefs ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Minimally Invasive ◽  
Tumor Growth ◽  
Hepatic Metastases ◽  
Specific Cell ◽  
Local Tumor ◽  
Orthotopic Mouse Model ◽  
Endoscopic Score ◽  
Local Tumor Growth

Open orthotopic mouse models of colorectal cancer have disadvantages such as the requirement for advanced surgical skills or the trauma caused by laparotomy. To overcome these drawbacks, this study aimed to evaluate the establishment of a minimally invasive model using murine colonoscopy. CT26 and MC38 CRC cells of different concentrations were injected into BALB/C and C57BL/6J mice, respectively. Follow-up endoscopies were performed to assign an endoscopic score to tumor growth. Gross autopsy, histologic and immuno-histochemical evaluation, and immune scoring were performed. To describe the learning curve of the procedures, a performance score was given. Local tumor growth with colorectal wall infiltration, luminal ulceration, the presence of tumor-infiltrating lymphocytes, lympho-vascular invasion, and early spontaneous lymph node, peritoneal, and hepatic metastases were observed. The tumors showed cytoplasmic immuno-staining for CK20. Compared to the MC38/C57BL/6J model, tumorigenicity and immunogenicity of the CT26/BALB/C model were higher. Tumor volume correlated with the endoscopic score. This endoscopy-guided orthotopic mouse model is easy to learn and quick to establish. It features early metastasis and enables the study of interactions with the immune system. When specific cell concentrations and cell lines are applied, controlled local tumor growth and metastasis can be achieved within short observation periods.

Curcumin sensitizes human colorectal cancer to capecitabine by modulation of cyclin D1, COX‐2, MMP‐9, VEGF and CXCR4 expression in an orthotopic mouse model

2009 ◽  
Vol 125 (9) ◽  
pp. 2187-2197 ◽  
Author(s):  
Ajaikumar B. Kunnumakkara ◽  
Parmeswaran Diagaradjane ◽  
Preetha Anand ◽  
Harikumar B. Kuzhuvelil ◽  
Amit Deorukhkar ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Cyclin D1 ◽  
Cxcr4 Expression ◽  
Human Colorectal Cancer ◽  
Orthotopic Mouse Model ◽  
Cox 2

scholarly journals The Nrf2 inhibitor brusatol is a potent antitumour agent in an orthotopic mouse model of colorectal cancer

Oncotarget ◽  
2018 ◽  
Vol 9 (43) ◽  
pp. 27104-27116 ◽  
Author(s):  
Jonathan P. Evans ◽  
Boleslaw K. Winiarski ◽  
Paul A. Sutton ◽  
Robert P. Jones ◽  
Lorenzo Ressel ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Orthotopic Mouse Model ◽  
Antitumour Agent

scholarly journals Correction: The Nrf2 inhibitor brusatol is a potent antitumour agent in an orthotopic mouse model of colorectal cancer

Oncotarget ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 685-685
Author(s):  
Jonathan P. Evans ◽  
Boleslaw K. Winiarski ◽  
Paul A. Sutton ◽  
Robert P. Jones ◽  
Lorenzo Ressel ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Orthotopic Mouse Model ◽  
Antitumour Agent

scholarly journals ILCs, the Frontliners of Immune Defense in Mouse Model of Colorectal Cancer

2021 ◽  
Author(s):  
Abolghasem Ajami ◽  
Mohsen keykhosravi ◽  
Seyed Mohammad javadzadeh ◽  
Mohsen Tehrani ◽  
hossein Asgarian-omran ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Mouse Models ◽  
Peripheral Blood ◽  
Sham Group ◽  
Immune Defense ◽  
Lymphoid Cells ◽  
Flow Cytometry Analysis ◽  
Orthotopic Mouse Model ◽  
Chemically Induced

Abstract Background and Objective: Innate lymphoid cells (ILCs) have been shown to play important roles in tumor immunity. We studied the frequency of three subsets of circulating ILCs in mouse models of colorectal cancer (CRC). Methods: Two mouse models of CRC were developed; including a chemically-induced model, via administration of azoxymethane/dextran sulfate sodium (AOM/DSS), and an orthotopic mouse model, using the CT-26 cell line. Based on histopathological examinations, mice were divided into 3 groups of dysplasia group (consists of chemically-induced and orthotopic induced), chemically-induced reparative change group, normal. A sham group was also considered in which mice were screened for stresses that originated from interventions and injections. Flow cytometry analysis was performed to evaluate the frequencies of ILC1, ILC2, and ILC3 in the peripheral blood of all studied mice.Results: The frequency of ILC1 was significantly higher in the chemically-induced reparative change group compared to the sham and dysplasia groups. ILC2s showed higher frequencies in the dysplasia groups than the sham and chemically-induced reparative change groups. In addition, altered composition of ILCs was observed in peripheral blood of dysplastic mice skewing toward ILC3s in the dysplasia groups compared to sham and chemically-induced reparative change groups. Conclusions: A higher frequency of ILC1 in the reparative change group suggests a potentially anti-tumorigenic role. Higher ILC2s might be in favor of differentiation from the reparative change stage to the dysplasia. In addition, it seems likely that ILC3s are participating in the primary stages of CRC development.

scholarly journals Establishment and Validation of an Orthotopic Metastatic Mouse Model of Colorectal Cancer

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Ashwani Rajput ◽  
Ekta Agarwal ◽  
Premila Leiphrakpam ◽  
Michael G. Brattain ◽  
Sanjib Chowdhury
Keyword(s):  
Colorectal Cancer ◽  
Liver Metastasis ◽  
Mouse Model ◽  
Model System ◽  
Metastatic Tumors ◽  
Orthotopic Mouse Model ◽  
Gene Signatures ◽  
Metastatic Colonization ◽  
Disseminated Disease

Metastases are largely responsible for cancer deaths in solid tumors due to the lack of effective therapies against disseminated disease, and there is an urgent need to fill this gap. This study demonstrates an orthotopic colorectal cancer (CRC) mouse model system to develop spontaneous metastasis in vivo and compare its reproducibility against human CRC. IGF1R-dependent GEO human CRC cells were used to study metastatic colonization using orthotopic transplantation procedures and demonstrated robust liver metastasis. Cell proliferation assays were performed both in the orthotopic primary colon and liver metastatic tumors, and human CRC patient’s specimen and similar patterns in H&E and Ki67 staining were observed between the orthotopically generated primary and liver metastatic tumors and human CRC specimens. Microarray analysis was performed to generate gene signatures, compared with deposited human CRC gene expression data sets, analyzed by Oncomine, and revealed similarity in gene signatures with increased aggressive markers expression associated with CRC in orthotopically generated liver metastasis. Thus, we have developed an orthotopic mouse model that reproduces human CRC metastasis. This model system can be effective in developing new therapeutic strategies against disseminated disease and could be implemented for identifying genes that regulate the development and/or maintenance of established metastasis.

Sign in / Sign up

Export Citation Format

Share Document