An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

10.3791/60790 ◽  
2020 ◽  
Author(s):  
Luz DC. Martínez-Sánchez ◽  
Rashmita Pradhan ◽  
Phuong A. Ngo ◽  
Lena Erkert ◽  
Lukas S. Becker ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Permeability ◽  
Intravital Microscopy ◽  
Cell Shedding

scholarly journals PWE-076 Absence Of Gut Microbiota Reduces Lipopolysaccharide-induced Epithelial Cell Shedding In The Small Intestine

Gut ◽  
2014 ◽  
Vol 63 (Suppl 1) ◽  
pp. A157.1-A157 ◽  
Author(s):  
K Hughes ◽  
C Alcon-Giner ◽  
M Lawson ◽  
K McCoy ◽  
A Macpherson ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cell ◽  
Gut Microbiota ◽  
Cell Shedding

Effects of neutrophil-derived oxidants on intestinal permeability, electrolyte transport, and epithelial cell viability

Inflammation ◽  
1990 ◽  
Vol 14 (5) ◽  
pp. 531-542 ◽  
Author(s):  
Matthew B. Grisham ◽  
Timothy S. Gaginella ◽  
Christoph von Ritter ◽  
Hiroshi Tamai ◽  
Robert M. Be ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Viability ◽  
Intestinal Permeability ◽  
Electrolyte Transport

scholarly journals γδ intraepithelial lymphocytes facilitate pathological epithelial cell shedding via CD103-mediated granzyme release.

2021 ◽  
Author(s):  
Madeleine D. Hu ◽  
Natasha B. Golovchenko ◽  
Grace L. Burns ◽  
Prema M. Nair ◽  
Thomas J. Kelly ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intraepithelial Lymphocytes ◽  
Cell Shedding

scholarly journals RSV-encoded NS2 promotes epithelial cell shedding and distal airway obstruction

2014 ◽  
Vol 124 (5) ◽  
pp. 2219-2233 ◽  
Author(s):  
Rachael M. Liesman ◽  
Ursula J. Buchholz ◽  
Cindy L. Luongo ◽  
Lijuan Yang ◽  
Alan D. Proia ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Airway Obstruction ◽  
Distal Airway ◽  
Cell Shedding

Expression of J1/tenascin in the crypt-villus unit of adult mouse small intestine: implications for its role in epithelial cell shedding

Development ◽  
1990 ◽  
Vol 109 (2) ◽  
pp. 313-321 ◽  
Author(s):  
R. Probstmeier ◽  
R. Martini ◽  
M. Schachner
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Basal Lamina ◽  
Adult Mouse ◽  
Epithelial Cell Line ◽  
Intestinal Epithelial ◽  
Collagen Types ◽  
Numerous Microvillus ◽  
Cell Shedding

The localization of the extracellular matrix recognition molecule J1/tenascin was investigated in the crypt-villus unit of the adult mouse ileum by immunoelectron microscopic techniques. In the villus region, J1/tenascin was detected strongly in the extracellular matrix (ECM) between fibroblasts of the lamina propria. It was generally absent in the ECM at the interface between subepithelial fibroblasts and intestinal epithelium, except for some restricted areas along the epithelial basal lamina of villi, but not of crypts. These restricted areas corresponded approximately to the basal part of one epithelial cell. In J1/tenascin-positive areas, epithelial cells contacted the basal lamina with numerous microvillus-like processes, whereas in J1/tenascin-negative areas the basal surface membranes of epithelial cells contacted their basal lamina in a smooth and continuous apposition. In order to characterize the functional role of J1/tenascin in the interaction between epithelial cells and ECM, the intestinal epithelial cell line HT-29 was tested for its ability to adhere to different ECM components. Cells adhered to substratum-immobilized fibronectin, laminin and collagen types I to IV, but not to J1/tenascin. When laminin or collagen types I to IV were mixed with J1/tenascin, cell adhesion was as effective as without J1/tenascin. However, adhesion was completely abolished when cells were offered a mixture of fibronectin and J1/tenascin as substratum. The ability of J1/tenascin to reduce the adhesion of intestinal epithelial cells to their fibronectin-containing basal lamina suggests that J1/tenascin may be involved in the process of physiological cell shedding from the villus.

scholarly journals Bacteroides fragilis toxin stimulates intestinal epithelial cell shedding and  -secretase-dependent E-cadherin cleavage

2007 ◽  
Vol 120 (20) ◽  
pp. 3713-3713
Author(s):  
S. Wu ◽  
K.-J. Rhee ◽  
M. Zhang ◽  
A. Franco ◽  
C. L. Sears
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Bacteroides Fragilis ◽  
Intestinal Epithelial ◽  
Cell Shedding ◽  
E Cadherin
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