Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

10.3791/62972 ◽  
2021 ◽  
Author(s):  
Ruby Boateng ◽  
Nathaniel Boechat ◽  
Philipp P. Henrich ◽  
Ewelina Bolcun-Filas
Keyword(s):  
Ovarian Reserve ◽  
Multiphoton Microscopy ◽  
3D Analysis ◽  
Whole Ovary

Direct phase determination in electron crystallography: small organic molecules

1992 ◽  
Vol 50 (2) ◽  
pp. 1166-1167
Author(s):  
Douglas L. Dorset
Keyword(s):  
Organic Molecules ◽  
Data Sets ◽  
Temperature Structure ◽  
3D Analysis ◽  
Intensity Data ◽  
Electron Crystallography ◽  
Phase Determination ◽  
Measured Intensity ◽  
3D Data

The quantitative use of electron diffraction intensity data for the determination of crystal structures represents the pioneering achievement in the electron crystallography of organic molecules, an effort largely begun by B. K. Vainshtein and his co-workers. However, despite numerous representative structure analyses yielding results consistent with X-ray determination, this entire effort was viewed with considerable mistrust by many crystallographers. This was no doubt due to the rather high crystallographic R-factors reported for some structures and, more importantly, the failure to convince many skeptics that the measured intensity data were adequate for ab initio structure determinations.We have recently demonstrated the utility of these data sets for structure analyses by direct phase determination based on the probabilistic estimate of three- and four-phase structure invariant sums. Examples include the structure of diketopiperazine using Vainshtein's 3D data, a similar 3D analysis of the room temperature structure of thiourea, and a zonal determination of the urea structure, the latter also based on data collected by the Moscow group.

A protocol to detect apoptotic dendritic cells in murine lymph nodes using multiphoton microscopy

2009 ◽  
Author(s):  
Cristina Delgado-Martn ◽  
Lorena Riol-Blanco ◽  
Luis M Alonso-C ◽  
Jos Luis Rodrguez-Fernndez
Keyword(s):  
Dendritic Cells ◽  
Lymph Nodes ◽  
Multiphoton Microscopy

Serum AMH concentration has limited prognostic value for the density of primordial follicles questioning AMH as a parameter for the real ovarian reserve

2018 ◽  
Author(s):  
J Liebenthron ◽  
J Reinsberg ◽  
R Fimmers ◽  
N Sänger ◽  
K van der Ven ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Prognostic Value ◽  
Primordial Follicles ◽  
The Real

Multiphoton microscopy

2015 ◽  
Author(s):  
Eleonora Dondossola ◽  
Peter Friedl
Keyword(s):  
Multiphoton Microscopy

Evidence for preserved ovarian reserve in transgender men receiving testosterone therapy: Anti-mullerian hormone serum levels decrease modestly after one year of treatment

2019 ◽  
Author(s):  
Iris Yaish ◽  
Gad Malinger ◽  
Foad Azem ◽  
Yael Sofer ◽  
Nechama Golani ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Serum Levels ◽  
Testosterone Therapy ◽  
One Year ◽  
Transgender Men

Long-term effects of childhood cancer treatment on hormonal and ultrasound markers of ovarian reserve

2019 ◽  
Author(s):  
van den Berg MH ◽  
Overbeek A ◽  
Lambalk CB ◽  
Kaspers GJL ◽  
Bresters D ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Childhood Cancer ◽  
Ovarian Reserve ◽  
Long Term Effects
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