Multiphoton microscopy

A protocol to detect apoptotic dendritic cells in murine lymph nodes using multiphoton microscopy

Protocol Exchange ◽

10.1038/nprot.2009.133 ◽

2009 ◽

Cited By ~ 1

Author(s):

Cristina Delgado-Martn ◽

Lorena Riol-Blanco ◽

Luis M Alonso-C ◽

Jos Luis Rodrguez-Fernndez

Keyword(s):

Dendritic Cells ◽

Lymph Nodes ◽

Multiphoton Microscopy

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Faculty Opinions recommendation of Combined non-linear laser imaging (two-photon excitation fluorescence microscopy, fluorescence lifetime imaging microscopy, multispectral multiphoton microscopy) in cutaneous tumours: first experiences.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1166974.629075 ◽

2009 ◽

Author(s):

Harvey Lui

Keyword(s):

Fluorescence Microscopy ◽

Fluorescence Lifetime ◽

Multiphoton Microscopy ◽

Fluorescence Lifetime Imaging Microscopy ◽

Fluorescence Lifetime Imaging ◽

Laser Imaging ◽

Two Photon ◽

Lifetime Imaging ◽

Photon Excitation ◽

Linear Laser

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Faculty Opinions recommendation of Angiotensin II AT2 receptor activation attenuates AT1 receptor-induced increases in the glomerular filtration of albumin: a multiphoton microscopy study.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718075985.793490514 ◽

2014 ◽

Author(s):

Neil Turner

Keyword(s):

Angiotensin Ii ◽

Glomerular Filtration ◽

Multiphoton Microscopy ◽

Microscopy Study ◽

Receptor Activation ◽

At1 Receptor ◽

At2 Receptor

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In Vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots

Nano Letters ◽

10.1021/acs.nanolett.9b01708 ◽

2019 ◽

Vol 19 (8) ◽

pp. 5260-5265 ◽

Cited By ~ 14

Author(s):

Hongji Liu ◽

Xiangquan Deng ◽

Shen Tong ◽

Chen He ◽

Hui Cheng ◽

...

Keyword(s):

Quantum Dots ◽

Multiphoton Microscopy ◽

Deep Brain

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Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

Molecules ◽

10.3390/molecules26123758 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3758

Author(s):

Andrea Ceja-Fdez ◽

Ramon Carriles ◽

Ana Lilia González-Yebra ◽

Juan Vivero-Escoto ◽

Elder de la Rosa ◽

...

Keyword(s):

Multiphoton Microscopy ◽

Surface Enhanced Raman Spectroscopy ◽

Rapid Screening ◽

Emission Region ◽

Sem Images ◽

Spectral Signatures ◽

Two Photon ◽

Surface Enhanced ◽

Surface Enhanced Raman ◽

Gold Nps

In this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and localization of the gold NPs inside of the two kinds of tissues. The light absorption of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was probably due to the aggregation of gold NPs inside the tissues. Therefore, through the interaction of gold nanoparticles with the HPV and cancerous cervical tissues, a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV-infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for the rapid screening of cancer.

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Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model

npj Flexible Electronics ◽

10.1038/s41528-021-00115-x ◽

2021 ◽

Vol 5 (1) ◽

Author(s):

Marie C. Lefevre ◽

Gerwin Dijk ◽

Attila Kaszas ◽

Martin Baca ◽

David Moreau ◽

...

Keyword(s):

Electric Field ◽

Electric Fields ◽

Flexible Electronics ◽

Soft Tissues ◽

Multiphoton Microscopy ◽

Membrane Integrity ◽

Tumor Model ◽

Pulsed Electric Fields ◽

In Ovo ◽

Cell Membrane Integrity

AbstractGlioblastoma is a highly aggressive brain tumor, very invasive and thus difficult to eradicate with standard oncology therapies. Bioelectric treatments based on pulsed electric fields have proven to be a successful method to treat cancerous tissues. However, they rely on stiff electrodes, which cause acute and chronic injuries, especially in soft tissues like the brain. Here we demonstrate the feasibility of delivering pulsed electric fields with flexible electronics using an in ovo vascularized tumor model. We show with fluorescence widefield and multiphoton microscopy that pulsed electric fields induce vasoconstriction of blood vessels and evoke calcium signals in vascularized glioblastoma spheroids stably expressing a genetically encoded fluorescence reporter. Simulations of the electric field delivery are compared with the measured influence of electric field effects on cell membrane integrity in exposed tumor cells. Our results confirm the feasibility of flexible electronics as a means of delivering intense pulsed electric fields to tumors in an intravital 3D vascularized model of human glioblastoma.

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