CSP plants using supercritical CO2 (sCO2) power cycle can potentially achieve high thermal conversion efficiency at low capital cost due to compact turbomachinery and other components. An sCO2 expander and improved heat exchanger is expected to provide a major stepping stone for achieving CSP power at $0.06/kW-hr LCOE, energy conversion efficiency > 50%, and total power block cost < $1,200/kW installed. However the life limiting mechanisms of these turbomachines in high pressure, high temperature sCO2 environment are not well understood. To understand the effect of high pressures, high temperatures and sCO2 chemical kinetics on crack initiation, crack propagation and low cycle fatigue (LCF) life of these turbomachines, a novel experimental setup is developed. Advanced microstructure and spectroscopic analyses are conducted that shed light on some key differences between various Ni base alloys in terms of oxidation morphology, chemical species diffusion and trapping, the formation of protective corrosion resistant layers and changes in surface properties. An experimental technique for low cycle fatigue experiments in high pressure, high temperature supercritical CO2 environment is developed. The test setup allows for pressurized LCF testing of alloys being considered for MW scale sCO2 turbine development. Results show that the LCF life remains the same (within the scatter band) irrespective of the location of crack initiation site whether at the OD (non shot-peened bars in air and sCO2), or at the ID (shot peened bars). Total fatigue life, for all conditions, lie within the normal variation in LCF results (± 2X life variation). No significant LCF life debit is observed in IN718 by sCO2 at 550 °C, 0.7% max strain, 20 cpm. Similar conclusion is reached during 0.6% max strain tests. The effect of sCO2 is found not to be significantly more damaging than air at these strain levels. However, the results can be different for lower % max strains due to longer exposure times involved, resulting from larger number of cycles to failure. Similarly at higher temperatures and/or longer hold-times, sCO2 environment may be more aggressive, resulting in lower total fatigue life.