The Prevalence of HPV Infection in General Population of Korea and Efficacy of HPV DNA Test as an Adjunctive Screening of Cervical Cancer

Objective: Persistence of high-risk HPV infection is known to be the major cause of cervical cancer. It is important to differentiate the genotype of HPV infection, whether it is high, intermediate or low risk. The aim of this study was to assess the prevalence of high-risk HPV types among Indonesian women with negative VIA. Method: We analyzed cervical swabs from 1,214 patients with negative VIA. By using INNO-Lipa HPV DNA test, we detected the HPV DNA and its genotype. Result: From the 1,214 women with negative VIA, 48 (3.95%) samples were confirmed to have positive HPV DNA by using PCR and electrophoresis. However, hybridization test were not able to detect HPV genotypes in 9 samples. These 9 samples were tested again with PCR and electrophoresis and resulted in negative HPV DNA. Among the remaining 39 samples (3.21%), we detected 19 types of HPV, consisting of 13 types of high-risk HPV, 5 types of low-risk HPV, and 1 type of unknown HPV (type X). Conclusion: Among patients with negative VIA, 3.21% was found to be positive for HPV DNA. From this percentage, the prevalence of high-risk HPV is higher than the low-risk and unknown HPV. Therefore we cannot ignore results of negative VIA, particularly in highrisk group, because there is a slight possibility that presence of HPV can be identified, especially the high risk ones which have a tendency to be persistent. We support the importance of HPV DNA test as cervical cancer screening method. [Indones J Obstet Gynecol 2014; 3: 153-156] Keywords: cervical cancer, high-risk HPV, negative VIA

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Visual inspection vs HPV DNA test: finding cost-effective options for cervical cancer screening in women living with HIV in Burkina Faso

PharmacoEconomics & Outcomes News ◽

10.1007/s40274-021-7646-7 ◽

2021 ◽

Vol 876 (1) ◽

pp. 32-32

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

Visual Inspection ◽

Cost Effective ◽

Dna Test ◽

Hpv Dna ◽

Women Living With Hiv ◽

Living With Hiv ◽

Test Finding ◽

Hpv Dna Test

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Efficiency of HPV DNA Test versus Pap Smear in the Screening of Cervical Cancer

Journal of Karnali Academy of Health Sciences ◽

10.3126/jkahs.v1i2.24128 ◽

2018 ◽

Vol 1 (2) ◽

pp. 9-13

Author(s):

Renee Pradhan ◽

U. Pant ◽

B. Aryal

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Pap Smear ◽

Intraepithelial Neoplasia ◽

Cervical Neoplasia ◽

Hpv Testing ◽

Dna Test ◽

Hpv Dna ◽

Smear Testing ◽

Hpv Dna Test

Introduction: Cancer cervix is a common genital cancer. Human papillomavirus is the main cause of cervical cancer because of the strong association of certain HPV genotypes and the development of cervical cancer and its precursor lesions, cervical intraepithelial neoplasia CIN 2 or CIN3. Methods: The study was conducted on 180 gynecological patients seen at the outpatient department of Manipal Hospital, Bangalore. A comparative study of HPV DNA test with Pap smear in the screening of cervical neoplasia was carried out over the period of 24 months from August 2011 to June 2013. Results: The incidence of cervical cancer and its associated mortality has declined in recent years, largely due to the widespread implementation of screening programs by Pap smear testing. The management and the prevention of cervical cancer should change with HPV DNA testing for high risk HPV, which is more sensitive than pap smear testing. Infection of cervix with HPV is necessary to cause cervical neoplasia and cervical cancer. Persistent infection with HPV is required for the development of cervical dysplasia and invasive cervical cancer. Conclusions: HPV testing alone for primary screening appears promising in women aged 30 years and older as this group is at greatest risk of developing CIN 3. As compared with Pap testing, HPV testing has greater sensitivity for detection of cervical intraepithelial neoplasia.

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The Evaluation of p16ink4a Immunoexpression/Immunostaining and Human Papillomavirus DNA Test in Cervical Liquid-Based Cytological Samples

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e3182009eea ◽

2011 ◽

Vol 21 (1) ◽

pp. 79-85 ◽

Cited By ~ 21

Author(s):

Maria Nasioutziki ◽

Angelos Daniilidis ◽

Kostos Dinas ◽

Maria Kyrgiou ◽

George Valasoulis ◽

...

Keyword(s):

High Risk ◽

Hpv Infection ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Dna Test ◽

Hpv Dna ◽

Squamous Cells ◽

Intraepithelial Lesion ◽

High Risk Hpv ◽

Hpv Dna Test

Aim:To evaluate the role of p16INK4a immunoexpression and human papillomavirus (HPV) DNA test for the detection of dyskaryotic cells in high-risk women.Materials and Methods:This work was a retrospective diagnostic study conducted in the University Hospital of Thessaloniki from January to December 2008. The subjects were women with current or previous HPV infection and current or previous cervical intraepithelial lesion (with or without treatment) or clinical warts. All liquid-based cytological samples were tested for P16INKa and HPV DNA test. The accuracy parameters used for the outcome included sensitivity, specificity, and positive predictive value.Results:A total of 226 women were included; the mean age was 29 years. Expression of p16INK4a was detected in the cytological samples of 13% of the negative cases, 44% of the cases of atypical squamous cells of undetermined significance, 46% of the cases of low-grade squamous intraepithelial lesion, and 78% of the cases of high-grade squamous intraepithelial lesion. A total of 91 women tested positive for high-risk HPV infection, and 54 of those had p16INK4a-positive staining reaction cells. The concordance between the 2 tests, HPV DNA and p16, was 59% regarding infection-positive cases. Diffuse strong parabasal p16INK4a immunostaining (nuclear score >2) was observed in 17 cases of the abnormal cytological findings (atypical squamous cells of undetermined significance, 2 cases; low-grade squamous intraepithelial lesion, 8 cases; high-grade squamous intraepithelial lesion, 7 cases). Colposcopy-directed biopsies were used as the criterion standard for the detection of cervical intraepithelial neoplasia in 91 women. The sensitivity of p16INK4a was 95% and the specificity was 92%, whereas the sensitivity of high-risk HPV was 100% and the specificity was 78%. The positive predictive value of p16INK4a was 71%, whereas that of HPV DNA was 44%.Conclusion:The findings suggest that p16INK4a immunostaining can improve the accuracy of cytological examination and HPV DNA test and may be particularly useful in the triage of low-grade lesions.

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Comparison of the Cepheid Xpert HPV test and the HC2 High-Risk HPV DNA Test for detection of high-risk HPV infection in cervical smear samples in SurePath preservative fluid

Journal of Medical Microbiology ◽

10.1099/jmm.0.000723 ◽

2018 ◽

Vol 67 (5) ◽

pp. 676-680 ◽

Cited By ~ 2

Author(s):

Ali A. Rabaan ◽

Shatha A. Alfaraj ◽

Mohammed A. Alkhalifah

Keyword(s):

High Risk ◽

Hpv Infection ◽

Cervical Smear ◽

Dna Test ◽

Hpv Dna ◽

Hpv Test ◽

High Risk Hpv ◽

Hpv Dna Test

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A Clinical Effectiveness of Cytology and HPV DNA test (Hybrid capture I) in Cervical Cancer Screening

Korean Journal of Gynecologic Oncology and Colposcopy ◽

10.3802/kjgoc.2002.13.4.342 ◽

2002 ◽

Vol 13 (4) ◽

pp. 342

Author(s):

Tae Gyu Ahn ◽

Kyoung Lee ◽

Dong Won Kim ◽

Sei Jun Han

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

Cervical Cancer Screening ◽

Clinical Effectiveness ◽

Hybrid Capture ◽

Dna Test ◽

Hpv Dna ◽

Hpv Dna Test

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Novel concepts in cervical cancer screening: a comparison of VIA, HPV DNA test and p16INK4a/Ki-67 dual stain cytology in Western Kenya

Infectious Agents and Cancer ◽

10.1186/s13027-020-00323-6 ◽

2020 ◽

Vol 15 (1) ◽

Cited By ~ 3

Author(s):

Elkanah Omenge Orang’o ◽

Edwin Were ◽

Oliver Rode ◽

Kapten Muthoka ◽

Michael Byczkowski ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

Cervical Cancer Screening ◽

Screening Program ◽

Hpv Infection ◽

High Morbidity ◽

Ki 67 ◽

Dna Test ◽

Hpv Dna ◽

Triage Test

Abstract Background Screening of unvaccinated women remains essential to mitigate the high morbidity/mortality of cervical cancer. Here, we compared visual inspection with acetic acid (VIA), recommended by WHO as the most cost-effective screening approach in LMICs, with HPV-based screening, and usage of p16INK4a/Ki-67 dual stain cytology. Methods We prospectively enrolled women participating in a VIA-based cervical cancer screening program in two peri-urban health centers of Kenya. Consenting women had a VIA examination preceded by collection of a liquid-based cytology sample from the cervix stored in PreservCyt medium (Hologic®). Analysis of all samples included a hrHPV DNA test and evaluation of a p16INK4a /Ki-67 (CINtecPLUS®) dual stained slide that was prepared using the ThinPrep® 2000 Processor and evaluated by a pathologist trained in the methodology. Results In 701 of a total of 800 women aged 18–64 years, all three investigations were performed and data could be analyzed. The HPV, VIA and dual stain cytology positivity were 33%, 7%, and 2% respectively. The HPV positivity rate of VIA positive cases was 32%. The five most common HPV types were HPV16, 52, 68, 58 and 35. The OR among HIV infected women of an HPV infection, VIA positivity and positive dual stain cytology were 2.6 (95%CI 1.5–4.3), 1.9 (95%CI 0.89–4.4) and 3.4 (95%CI 1.07–10.9) respectively. The sensitivity of VIA to detect a p16INK4a/Ki-67 positive transforming infection was 13% (95%CI 2–38). Conclusions Primary HPV testing appears feasible and should be considered as a primary screening test also in LMICs. The poor sensitivity of VIA renders it unsuitable as a triage test for HPV positive women. The utility of p16INK4a/Ki-67 dual stain cytology as a triage test for HPV positive women in LMICs should be further studied.

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