Influence of Peloid Therapy on the Condition and Functional Activity of the Glands of Internal Secretion in Juvenile Rheumatoid Arthritis (Clinical and Experimental Study)

2020 ◽  
Vol 98 (4) ◽  
pp. 82-89
Author(s):  
N.N. Kaladze ◽  
E.Y. Zyablitskaya
Keyword(s):  
Rheumatoid Arthritis ◽  
Thyroid Gland ◽  
Juvenile Rheumatoid Arthritis ◽  
Functional Activity ◽  
Adjuvant Arthritis ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Thyroid Axis ◽  
Peloid Therapy ◽  
Positive Effect

The aim of the study was to assess the dynamics of hormones of the pituitary-gonadal-thyroid axis in patients with juvenile rheumatoid arthritis under the influence of peloid therapy and to establish the corresponding morphological changes in the pituitary gland, gonads and the thyroid gland of white Wistar rats with induced adjuvant arthritis during peloid therapy. Material and methods. 46 patients with juvenile rheumatoid arthritis were examined during their rehabilitation at sanatoria. The effect of peloid therapy on the pituitary-gonadal-thyroid axis was evaluated based on the changes in the levels of the main hormones LH, FSH, estradiol, testosterone, TSH, T3, T4. The control group consisted of 15 healthy age-mates. Morphological changes in the pituitary, testes, ovaries and thyroid gland were studied in 24 white Wistar rats with adjuvant arthritis. Results. In juvenile rheumatoid arthritis, the pituitary-gonad-thyroid axis is activated, which is manifested by a decrease in the production of FSH, LH, TSH, testosterone in boys of all age groups and estradiol both in girls regardless of their age and in boys 12–17 years of age. At the same time, an increase in secretion of T3, T4 was observed. The most pronounced deviations were noted in patients with a systemic form of the disease, with a high degree of severity and duration of the disease. The inclusion of mud therapy into the program of sanatorium rehabilitation leads to positive changes in the levels of the studied hormones. The experiment with induced adjuvant arthritis revealed reversible ultrastructural and tinctorial changes in the thyrotropocytes and gonadotropocytes of the pituitary gland, as well as impaired differentiation and partial destruction of the structural elements in the thyroid gland and gonads. The course of peloid therapy had a positive effect on the condition of the thyroid gland and gonads in the experimental animals, which was manifested by the restoration of structural and functional organization. Conclusion. Peloid therapy has a positive effect on the functional activity of the pituitary-gonadal thyroid axis in patients with juvenile rheumatoid arthritis, which has been confirmed by experimental data.

scholarly journals Hormones of thyroid gland in sera of rats treated with different dose of concentrated potassium iodine solutions

2010 ◽  
Vol 138 (5-6) ◽  
pp. 323-327 ◽  
Author(s):  
Ljiljana Markovic ◽  
Violeta Mihailovic-Vucinic ◽  
Jelena Aritonovic
Keyword(s):  
Thyroid Gland ◽  
Genetic Susceptibility ◽  
Experimental Model ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Cell Necrosis ◽  
High Iodine ◽  
Hormone Status ◽  
Potassium Iodine ◽  
Iodine Dose

Introduction Potassium iodine (KI) is used as a drug therapy for treating numerous diseases such as small-vessel vasculitis, erythema nodosum, vasculitis nodularis, Sweet's syndrome, tuberculosis and granulomatosis, and for iodized salt. At the same time, KI can be harmful. Iodine intake may increase the frequency of thyroiditis in humans, and may induce the occurrence of experimental thyroiditis (ET) in animals. Investigations on an experimental model for the examination of thyroiditis in Wistar rats have clearly showed morphological changes in the rat thyroid evoked by KI administration. Objective The purpose of this study was to compare the effects of low and high doses of KI on the thyroid gland of Wistar rats and determine the effect on hormone status (T4, T3 and TSH) in this rat strain. Methods Two groups of rats from the Wistar strain were treated with a low iodine dose (225 ?g/g BW) and with a high iodine dose (675 ?g/g BW) of KI solutions. Untreated nonimmunized animals served as controls. The solution was administrated daily intraperitoneally during the period of 26 consecutive days. Results Monitoring hormone status (TSH, T3 and T4) and morphological changes it was found that therapeutic doses of KI applied in treatment induced the occurrence of experimental thyroiditis (chronic destructive Hashimoto's thyroiditis in humans) and cell necrosis in animals not carrying a genetic susceptibility. Significant inflammatory changes were observed in rats treated with a high iodine dose. Conclusion The early iodine induced cell necrosis and inflammation in the nonimmunized animals without genetic susceptibility is a new experimental model of thyroiditis. .

scholarly journals INFLUENCE OF ALIMENTARY DEPRIVATION ON MORPHOLOGICAL CHANGES OF RAT'S THYROID GLAND

2021 ◽  
Vol 26 (2(49)) ◽  
pp. 89-97
Author(s):  
R. V. Yanko
Keyword(s):  
Thyroid Gland ◽  
Functional Activity ◽  
Morphological Changes ◽  
Thyroid Tissue ◽  
Digital Camera ◽  
The Body ◽  
Standard Diet ◽  
Thyroid Cells ◽  
Experimental Animals ◽  
The Impact

Introduction: Despite the well-studied effect of alimentary deprivation on the body, the literature data on its effect on functional activity and, in particular, on morphological changes in the thyroid gland are single and often contradictory, which does not allow unambiguous conclusions. All this requires a more detailed study of the role and mechanisms of the impact of restricted nutrition on the thyroid gland. Aim: To investigate the effect of alimentary deprivation on morphological changes in the thyroid gland of young rats. Methods: The study was conducted on 24 male Wistar rats aged 3 months. Rats of all groups were in uniform conditions, on a standard diet. Animals of the experimental group, for 28 days, received a diet reduced by 30 %. Work with rats was carried out in accordance with the principles of the Declaration of Helsinki. Histological preparations were made from the central areas of the thyroid tissue according to the standard method. Using a digital camera, the micropreparations were photographed under a Nikon Eclipse E 100 microscope (Japan). Morphometry was performed using a computer program "Image J". Results: Histological analysis of the rat's thyroid gland affected by alimentary deprivation revealed that it had an unchanged physiological structure. The follicles were mostly of oval shape and of various sizes. Colloid in the follicles of experimental animals is of moderate density and contains numerous resorption vacuoles. Thyroid cells are of prismatic and cubic shape. It was found that in the thyroid gland of experimental rats the area of ​​follicles, colloid, their inner diameter decreases, the height of thyrocytes increases, the stereological resorption index increases and the colloid accumulation index decreases, the number of interfollicular islands increases. Also in experimental animals there was a decrease in the width of the interlobar and interfollicular connective tissue. Conclusion: In rats fed on a reduced diet, morphological signs of increased functional activity of the thyroid gland were found.

scholarly journals INDICATORS OF ТНЕ FUNCTIONALACTIVITY OF ТНЕ THYMUS, STEM CELLS AND APOPTOSIS МARКERS IN PATIENTS WITH JUVENILE RHEUМATOID ARTHRITIS АТ ТНЕ SANATORIUM STAGE OF REНABILITATION

2020 ◽  
Vol 26 (3) ◽  
pp. 96-99
Author(s):  
Е. М. Soboleva ◽  
N N. Kaladze
Keyword(s):  
Rheumatoid Arthritis ◽  
Stem Cells ◽  
Juvenile Rheumatoid Arthritis ◽  
Functional Activity ◽  
Systemic Circulation ◽  
Control Group ◽  
Stem Cell Markers ◽  
Differentiated Cells ◽  
Apoptosis Markers ◽  
Poorly Differentiated

The aim of the study was to evaluate the effect of sanatorium treatment on the indicators of the functional activity of the thymus, stem cells and apoptosis markers in patients with juvenile rheumatoid arthritis. Material and methods. 48 patients with juvenile rheumatoid arthritis were examined. The effect of sanatorium rehabilitation on the functional activity of the thymus was evaluated by the changes in the release of its main hormone, α1-thymosin, as well as of markers of stem cells (CD34) and apoptosis (CD95). The control group consisted of 15 healthy peers. Results. In juvenile rheumatoid arthritis, the functional activity of the thymus decreases, which is manifested by significantly lower levels of secretion of its main hormone α1-thymosin, which can contribute to the disruption of the processes of T-lymphocyte selection and the appearance of autoreactive clones. There was also a significant increase in stem cell markers (CD34) and markers of the readiness of activated lymphocytes for apoptosis (CD95). This fact indicates the accumulation of poorly differentiated cells in the systemic bloodstream and impaired apoptosis. Sanatorium treatment leads to a significant increase in α1-thymosin and positive changes in CD34 and CD95 levels. Conclusion. The factors contributing to the development of an autoimmune process were identified in patients with juvenile rheumatoid arthritis. A decrease in thymus α1-thymosin, combined with an increased level of CD34 and CD95, can contribute to the impairment of negative selection of T-lymphocytes in the thymus, accumulation of autoreactive clones and poorly differentiated cells that have not undergone apoptosis in the systemic circulation. Sanatorium treatment has a normalizing effect on the parameters under study.

scholarly journals EFFECT OF CHRONIC NEUROGENIC PAIN ON FUNCTIONAL ACTIVITY OF THYROID GLAND IN MALE MICE WITH TRANSPLANTABLE B16/F10 MELANOMA

2020 ◽  
Vol 66 (4) ◽  
pp. 434-438
Author(s):  
Oleg Kit ◽  
Yelena Frantsiyants ◽  
Valeriya Bandovkina ◽  
Irina Kaplieva ◽  
Inga Kotieva ◽  
...  
Keyword(s):  
Thyroid Gland ◽  
Thyroid Hormones ◽  
Functional Activity ◽  
Weight Coefficient ◽  
Male Mice ◽  
Neurogenic Pain ◽  
Total Triiodothyronine ◽  
Thyroid Axis ◽  
Exogenous Factors ◽  
Modifying Effect

The important role of the thyroid axis in the body's response to various endogenous and exogenous factors is beyond doubt. The data on the effect of tumor growth on the functional activity of the thyroid gland are inconsistent. A modifying effect of chronic neurogenic pain on the biological aggressiveness of transplantable B16/F10 melanoma requires studying the main mechanisms of action. The aim of the study was to analyze the effect of chronic neurogenic pain on the functional activity of the thyroid gland in the dynamics of B16/F10 melanoma growth in male mice. Methods. The comparison group included animals with standard subcutaneous transplantation of B16/F10 melanoma; the main group - mice with B16/F10 melanoma transplanted 2 weeks after the generation of the chronic neurogenic pain model. Levels of total and free forms of thyroid hormones and TSH were determined in thyroid cytosolic fractions by standard ELISA methods. Results. Chronic neurogenic pain increases the levels of free forms of thyroid hormones and total triiodothyronine, but decreases the levels of TSH and total thyroxin in the thyroid, as well as the organ weight coefficient in male mice with transplanted B16/F10 melanoma. Conclusions. The results demonstrated that the thyroid activity was a point of application of modifying influence of chronic neurogenic pain on the course of transplantable B16/F10 melanoma in male mice.

scholarly journals REHABILITATION MEASURES OF DENTAL DIRECTION IN PATIENTS WITH JUVENILE RHEUMATOID ARTHRITIS IN SANATORIUM-RESORT CONDITIONS

2018 ◽  
Vol 17 (2) ◽  
pp. 76-81
Author(s):  
N. N Kaladze ◽  
Olga Petrovna Galkina ◽  
S. G Bezrukov
Keyword(s):  
Rheumatoid Arthritis ◽  
Salivary Glands ◽  
Juvenile Rheumatoid Arthritis ◽  
Dental Care ◽  
Functional Activity ◽  
Oral Fluid ◽  
Physiological Activity ◽  
Health Effect ◽  
Natural Factors ◽  
Pathological Focus

Physiotherapeutic procedures normalize physicochemical processes at the cellular and molecular levels. The complex application of natural factors for reducing the interference of the leading pathological focus and deviations of other localization, including the maxillofacial region is actual. The purpose of the study is the development of a therapeutic and prophylactic dental complex for patients with juvenile rheumatoid arthritis (JRA) staying in sanatorium. Material and methods. 30 patients with JRA in the Evpatoriya were examined. Patients of the comparison group receved standart therapy. Patients of the main group additionally received bioresonance stimulation (BRS) of the collar zone and projection of large salivary glands; the oral cavity was rinsed with a solution of the brine from Saky’s Lake. Dental indices, rate of salivation, saliva viscosity, cortisol and ACTH in oral fluid and blood were determined. Results. The effectiveness of dental care in resort conditions was noted in both groups, but the reduction of rate of salivation and viscosity of oral fluid was more dynamic (on 31,58% and 15,6%), reflecting the ability of the BRS to regulate functional activity salivary glands. Cortisol in the OF decreased, which indirectly indicated the physiological activity of the excretory apparatus of the salivary glands at the level of transport molecular metabolism. Conclusions. Staying patients with JRA in the sanatorium-resort conditions in Evpatoriya has a general health effect. “Small” balneotherapy of the brine from Saky’s Lake improves oral hygiene. BRS-therapy restores the functional activity of salivary glands, promotes regulation of cortisol and ACTH levels. Providing rehabilitation dental care for patients with JRA, in the conditions of the resort is justified.

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