Molecular Detection of Drug resistant and Non Tuberculosis Mycobacteria using Line Probe Assay: A case study from North India

2020 ◽  
Keyword(s):  
Molecular Detection ◽  
North India ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Probe Assay

scholarly journals Evaluation of MTBDRplusand MTBDRslin Detecting Drug-Resistant Tuberculosis in a Chinese Population

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Wei Lu ◽  
Yan Feng ◽  
Jianming Wang ◽  
Limei Zhu
Keyword(s):  
Drug Resistance ◽  
Chinese Population ◽  
Sequencing Analysis ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Drug Resistant Tuberculosis ◽  
Additional Tool ◽  
Resistant Tuberculosis ◽  
Probe Assay ◽  
Mycobacteria Tuberculosis

Background. This study aims to evaluate GenoType MTBDRplusand GenoType MTBDRslfor their ability to detect drug-resistant tuberculosis in a Chinese population.Methods. We collected 112Mycobacteria tuberculosisstrains from Jiangsu province, China. The conventional DST and line probe assay were used to detect drug resistance to rifampicin (RFP), isoniazid (INH), ofloxacin (OFX), kanamycin (Km), and ethambutol (EMB).Results. The sensitivity and specificity were 100% and 50% for RFP and 86.11% and 47.06% for INH, respectively. The most common mutations observed in MTBDRpluswererpoBWT8 omission + MUT3 presence,katGWT omission + MUT1 presence, andinhAWT1 omission + MUT1 presence. For drug resistance to OFX, Km, and EMB, the sensitivity of MTBDRslwas 94.74%, 62.50%, and 58.82%, respectively, while the specificity was 92.59%, 98.81%, and 91.67%, respectively. The most common mutations weregyrAWT3 omission + MUT3C presence,rrsMUT1 presence,embBWT omission + MUT1B presence, andembBWT omission + MUT1A presence. Sequencing analysis found several uncommon mutations.Conclusion. In combination with DST, application of the GenoType MTBDRplusand GenoType MTBDRslassays might be a useful additional tool to allow for the rapid and safe diagnosis of drug resistance to RFP and OFX.

scholarly journals Comparison of GeneXpert and Line Probe Assay for Detection of Mycobacterium tuberculosis and Rifampicin-Mono resistance at the National Tuberculosis Reference Laboratory, Kenya

2019 ◽  
Author(s):  
S.A. Aricha ◽  
Leonard King'wara ◽  
N.W. Mwirigi ◽  
Linda Chaba ◽  
T. Kiptai ◽  
...  
Keyword(s):  
Predictive Value ◽  
Performance Characteristics ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Perfect Agreement ◽  
Predictive Values ◽  
National Tuberculosis ◽  
Probe Assay ◽  
Sensitivity Specificity

Abstract Introduction The dual challenge of low diagnostic sensitivity of microscopy test and technical challenge of performing a TB culture test poses a problem for case detection and initiation of Tuberculosis (TB) second-line treatment. There is thus need for a rapid, reliable and easily accessible assay. This comparative analysis was performed to assess diagnostic performance characteristics of GeneXpert MTB/RIF and Line Probe Assay (LPA) Methods 329 sputum samples of patients across the 47 counties in Kenya suspected to have drug resistant TB were picked and subjected to GeneXpert, LPA and Culture MGIT at the National TB Reference Laboratory. Sensitivity, specificity and predictive values were then determined to assess the performance characteristics of various assays. Results GeneXpert had a sensitivity, specificity, positive predictive value, and negative predictive value of 78.5%, 64.9%, 59.4% and 82.2% respectively while LPA had 98.4%, 66.0%, 65.4% and 98.4%. For diagnosis of rifampicin mono-resistance GeneXpert had a moderate agreement (Kappa=0.59, P<0.01) (sensitivity= 62.50%, specificity = 96.50%) while LPA that had almost perfect agreement (Kappa= 0.89, p<0.01) with a (sensitivity= 90.0% and specificity= 99.1%). Conclusion LPA has a better performance characteristic to GeneXpert and an alternative to culture with regards to detection of RIF’s mono-resistance.

scholarly journals RAPID DETECTION OF MULTI DRUG RESISTANCE AMONG MULTI DRUG RESISTANT TUBERCULOSIS SUSPECTS USING LINE PROBE ASSAY

2016 ◽  
Vol 10 (1) ◽  
pp. 131
Author(s):  
Sanjeev Saini ◽  
Manoj Kumar Dubey ◽  
Uma Bhardwaj ◽  
M Hanif ◽  
Chopra Kk ◽  
...  
Keyword(s):  
Fluorescent Microscopy ◽  
Multi Drug Resistance ◽  
Sputum Smear ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Multi Drug Resistant Tuberculosis ◽  
Mdr Tb ◽  
Probe Assay

ABSTRACTObjective: GenoType MTBDRplus line probe assay (LPA) is developed for performing drug susceptibility testing (DST) for Rifampicin (RIF) andisoniazid in sputum specimens from smear-positive pulmonary tuberculosis (TB) patients and revised national TB control Programme (RNTCP)has endorsed LPA for the diagnosis of multi drug resistant TB (MDR-TB). This study was conducted to assess the potential utility of LPA for MDR-TBpatient management.Methods: MDR-TB suspects under RNTCP PMDT criteria C referred from different districts in Delhi state were included in the study January 2013 toDecember 2014. Sputum specimens found acid-fast bacilli positive by fluorescent microscopy were processed for LPA.Results: Out of 3062 specimens, 2055 (67.1%) MDR-TB suspects were read as positive and specimens from 1007 (32.9%) suspects were read asnegative in sputum smear microscopy. Out of 2019 specimens valid LPA results, 1427 were found to be pan-sensitive, 280 were MDR-TB, 40 were RIFmonoresistant, 183 were Isoniazid (INH) monoresistant, and 89 specimens were found negative for Mycobacterium tuberculosis.Conclusion: Routine use of LPA can substantially reduce the time to diagnosis of RIF and/or INH-resistant TB and can hence potentially enable earliercommencement of appropriate drug therapy and thereby facilitate prevention of further transmission of drug resistant strains.Keywords: Multi drug resistant tuberculosis, Line probe assay, Rifampicin, Isoniazid.

scholarly journals Comparison of GeneXpert and Line Probe Assay for Detection of Mycobacterium tuberculosis and Rifampicin-Mono resistance at the National Tuberculosis Reference Laboratory, Kenya

2019 ◽  
Author(s):  
S.A. Aricha ◽  
Leonard King'wara ◽  
N.W. Mwirigi ◽  
Linda Chaba ◽  
T. Kiptai ◽  
...  
Keyword(s):  
Predictive Value ◽  
Performance Characteristics ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Perfect Agreement ◽  
Predictive Values ◽  
National Tuberculosis ◽  
Probe Assay ◽  
Sensitivity Specificity

Abstract Background: The dual challenge of low diagnostic sensitivity of microscopy test and technical challenge of performing a TB culture test poses a problem for case detection and initiation of Tuberculosis (TB) second-line treatment. There is thus need for a rapid, reliable and easily accessible assay. This comparative analysis was performed to assess diagnostic performance characteristics of GeneXpert MTB/RIF and Line Probe Assay (LPA) Methods: 329 sputum samples of patients across the 47 counties in Kenya suspected to have drug resistant TB were picked and subjected to GeneXpert, LPA and Culture MGIT at the National TB Reference Laboratory. Sensitivity, specificity and predictive values were then determined to assess the performance characteristics of the various assays. Results: Against culture MGIT as the gold standard for TB diagnosis, GeneXpert had a sensitivity, specificity, positive predictive value, and negative predictive value of 78.5%, 64.9%, 59.4 % and 82.2% respectively while LPA had 98.4%, 66.0 %, 65.4% and 98.4%. For diagnosis of rifampicin mono-resistance GeneXpert had a moderate agreement (Kappa=0.59, P<0.01) (sensitivity= 62.50%, specificity = 96.50 %) while LPA that had almost perfect agreement (Kappa= 0.89, p<0.01) with a (sensitivity= 90.0% and specificity= 99.1%). Conclusion: LPA has a better performance characteristic to GeneXpert and an alternative to culture with regards to detection of RIF’s mono-resistance. Keywords: LPA, GeneXpert, Sensitivity, Specificity, Drug-resistant TB

scholarly journals Comparison of GeneXpert and Line Probe Assay for Detection of Mycobacterium tuberculosis and Rifampicin-Mono resistance at the National Tuberculosis Reference Laboratory, Kenya

2019 ◽  
Author(s):  
S.A. Aricha ◽  
Leonard King'wara ◽  
N.W. Mwirigi ◽  
Linda Chaba ◽  
T. Kiptai ◽  
...  
Keyword(s):  
Predictive Value ◽  
Performance Characteristics ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Line Probe Assay ◽  
Perfect Agreement ◽  
Predictive Values ◽  
National Tuberculosis ◽  
Probe Assay ◽  
Sensitivity Specificity

Abstract Background: The dual challenge of low diagnostic sensitivity of microscopy test and technical challenge of performing a TB culture test poses a problem for case detection and initiation of Tuberculosis (TB) second-line treatment. There is thus need for a rapid, reliable and easily accessible assay. This comparative analysis was performed to assess diagnostic performance characteristics of GeneXpert MTB/RIF and Line Probe Assay (LPA) Methods : 329 sputum samples of patients across the 47 counties in Kenya suspected to have drug resistant TB were picked and subjected to GeneXpert, LPA and Culture MGIT at the National TB Reference Laboratory. Sensitivity, specificity and predictive values were then determined to assess the performance characteristics of the various assays. Results: Against culture MGIT as the gold standard for TB diagnosis, GeneXpert had a sensitivity, specificity, positive predictive value, and negative predictive value of 78.5%, 64.9%, 59.4 % and 82.2% respectively while LPA had 98.4%, 66.0 %, 65.4% and 98.4%. For diagnosis of rifampicin mono-resistance GeneXpert had a moderate agreement (Kappa 0.59, P<0.01) (sensitivity 62.50%, specificity 96.50 %) while LPA that had almost perfect agreement (Kappa=0.89, p<0.01) with a (sensitivity 90.0% and specificity 99.1%). Conclusion : LPA has a better performance characteristic to GeneXpert and an alternative to culture with regards to detection of RIF’s mono-resistance. Keywords : LPA, GeneXpert, Sensitivity, Specificity, Drug-resistant TB

Sign in / Sign up

Export Citation Format

Share Document