Camel milk modulates α-lactalbumin induced changes in the gut microbiota in a murine model of cow’s milk allergy

Camel milk has been noted to have medicinal properties treating allergies, diarrhea, microbial infection and Crohn's disease.The aim of this study is to identify and characterize the intestinal microbiota of BALB/c mice sensitized to α-lactalbumin (α-la) and fed with camel milk. For this, 30 females BALB/c mice aged between 4 and 6 weeks were divided into two groups. The first one designated as control group did not receive any treatment (n=10). The second group (n=20) was immunized intraperitoneally with α-la. On day 35 of sensitization protocol, mice of the second group were divided into two subgroups of 10 mice each. Animals of the first subgroup were sacrificed, whereas, mice of the second subgroup were fed camel milk for one week. In order to analyze the effect of camel milk on α-La-sensitized mice, two studies were carried out: a histological analysis of the jejunal tissues and microbiolo-gical study in the intestine and faeces of mice of different groups. Our results showed that drinking camel milk significantly reduced the alteration of in-testinal epithelium induced by α-la immunization as demonstrated by a signi-ficant increase of villus height (p<0.05). The microbiological results showed a very significant increase in enterobacteria and anaerobic bacteria (1 to 2 log ufc/g) in bacterial flora of mice immunized to α-la. However there was no significant difference in the level of enterobacteriaceae and anaerobic bacte-ria in the faeces of α-la-immunized mice treated with camel milk. In conclu-sion, Camel milk reduced the microscopic lesions caused by α-lasensitization and play an antibacterial role againstpathogenic bacteria in the intestinal mucosa of mice immunized to cow’s milk proteins especially α-la.

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Faculty Opinions recommendation of Contribution of IgE and immunoglobulin free light chain in the allergic reaction to cow's milk proteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717971166.793469473 ◽

2013 ◽

Author(s):

Rob Stockley

Keyword(s):

Allergic Reaction ◽

Light Chain ◽

Milk Proteins ◽

Free Light Chain ◽

Cow’S Milk ◽

Cow's Milk ◽

Cow’S Milk Proteins

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Alergia à Proteína do Leite de Vaca Persistente em Adulto: Relato de Caso / Persistent Cow’s Milk Allergy in Adult: Case Report

REVISTA CIÊNCIAS EM SAÚDE ◽

10.21876/rcsfmit.v5i4.400 ◽

1970 ◽

Vol 5 (4) ◽

pp. 51-60

Author(s):

José Henrique Pereira Pinto ◽

Renan Lemos de Toledo ◽

William do Prado Franquelo

Keyword(s):

Immune Response ◽

Case Report ◽

Inflammatory Disease ◽

Clinical Manifestations ◽

Milk Proteins ◽

Cow’S Milk ◽

Cow's Milk ◽

Current Prevalence ◽

Keywords Allergy ◽

Ige Mediated

RESUMOIntrodução: Alergia à Proteína do Leite de Vaca (APLV) é uma doença inflamatória secundária à reação imunológica contra uma ou mais proteínas do leite de vaca (LV) que afeta principalmente a faixa pediátrica. A real prevalência é discutida em muitos estudos. As manifestações clínicas dependem do tipo da resposta imunológica, ser IgE mediada ou não. Os sintomas se iniciam por volta dos 06 meses de vida e na maioria dos casos, esse processo alérgico regride, com o paciente desenvolvendo tolerância até a adolescência. Casuística: Relata-se um caso de um paciente do sexo masculino, apresentando desde os 6 meses de idade de anafilaxia e broncoespasmo. Nesta época foi levado em hospitais e ambulatórios sendo diagnosticado e tratado como asma apenas, porém sem sucesso. Aos 18 anos, em consulta com especialista foi diagnosticado com APLV, apesar da dieta de exclusão, apresentou diversas reações anafiláticas, devido a ingestão acidental do alérgeno. Discussão: O paciente iniciou os primeiros sintomas quando houve contato com LV e apresentou teste laboratorial com valores compatíveis a patologia. Segundo a literatura a prevalência de APLV cai para menos de 1% aos 6 anos de vida e está persistência pode estar associada a múltiplos fatores, no caso relatado, o paciente não apresentou tolerância até o presente momento. Conclusão: APLV é uma doença usualmente de criança em que, se estas não adquirirem tolerância, complicações podem perdurar indefinidamente. O Diagnóstico precoce e o manejo adequado desta condição, revela grande importância na qualidade de vida e na prevenção de anafilaxia.Palavras chave: Alergia, Proteína do leite de vaca, Anafilaxia. ABSTRACT Introduction: Allergy to cow's milk (CMPA) is an inflammatory disease Introduction: Allergy to cow's milk (CMPA) is an inflammatory disease secondary to immune response against one or more cow's milk proteins (LV) which primarily affects pediatric patients. The current prevalence is discussed in many studies. The clinical manifestations depend on the type of immune response, being IgE mediated or not. Symptoms start at about 06 months of life and in most cases, the allergic process subsides, and the patient develops tolerance through adolescence. Case Report: We report the case of a male patient, who was presenting, since his 06 months of age, anaphylaxis and bronchospasm. At that time he was taken into hospitals and clinics being diagnosed and treated as asthma, but without success. At 18, in consultation with expert was diagnosed with CMPA, and despite the exclusion diet, presented several anaphylactic reactions due to accidental ingestion of the allergen. Discussion: The patient began the first symptoms when there was contact with LV and presented laboratory test values compatible with the pathology. According to the literature the prevalence of CMPA drops to less than 1% to 6 years of life and this persistence can be associated with multiple factors, in our case, the patient did not develop tolerance to date. Conclusion: CMPA is usually a child disease but ,if they do not acquire tolerance, complications can last indefinitely. Early diagnosis and appropriate management of this condition, reveals a great deal on quality of life and prevention of anaphylaxis. Keywords: Allergy, Cow’s milk protein, Anaphylaxis.

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Soy Formula Doesn't Help Colic

PEDIATRICS ◽

10.1542/peds.71.2.299b ◽

1983 ◽

Vol 71 (2) ◽

pp. 299-300

Author(s):

LASSE LOTHE ◽

TOR LINDBERG ◽

IRÉNE JAKOBSSON

Keyword(s):

Adverse Reaction ◽

Soy Protein ◽

Milk Proteins ◽

Cow’S Milk ◽

Milk Formula ◽

Cow's Milk ◽

Cow’S Milk Proteins

In Reply.— We fully agree with LeBlanc that there was no significant improvement when infants with colic were given a soy protein-based formula. As pointed out in the "Discussion," as many as 53% of the infants showed an adverse reaction to soy (corresponding figure for cow's milk formula was 71%). We also emphasized that these figures must be interpreted with caution. In fact, soy protein-based formula was a bad choice as placebo. A placebo substance should be a substance of no importance as an allergen in infancy and soy protein has been shown to be as antigenic as cow's milk proteins (Eastham EJ, et al: J Pediatr 1978;93:561).

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ALLERGENIC ACTIVITY AND DANGER OF INDUSTRIAL DUSTS OF DRY PRODUCTS OF COW'S MILK PROCESSING

Medical Journal ◽

10.51922/1818-426x.2021.4.51 ◽

2021 ◽

pp. 51-55

Author(s):

Baranov S.A. ◽

◽

Shevlyakov V.V. ◽

Sychyk S.I. ◽

Filonyuk V.A. ◽

...

Keyword(s):

Milk Proteins ◽

The Body ◽

Cow’S Milk ◽

Antigenic Determinants ◽

Allergic Reactions ◽

Cow's Milk ◽

Milk Processing ◽

Allergenic Activity

The purpose of the work was to establish in a model experiment the allergenic activity and danger of the extracts obtained from the dust of dry products of cow's milk processing (DPMP), containing complexes of soluble whey (WMP) or casein milk proteins (CMP), as a stage of hygienic regulation of the content of dust DPMP in the air of the working area. Experiments on albino guinea pigs sensitized by the intradermal injection of standard doses of WMP and СМР solutions into the ear revealed the development of severe allergic reactions in the animals of the experimental groups with the prevalence of mixed mechanisms of immediate anaphylactic and delayed cell-mediated types. According to the criteria for the classification of industrial allergens, the WMP and СМР complexes have a strong allergenic activity and are differentiated to the 1-st class of allergenic hazard, which determines the classification of the DPMP dust containing them as extremely dangerous industrial allergens. This is confirmed by the established high levels of indicators of allergic-diagnostic reactions in vivo and in vitro when testing sensitized WMP and СМР animals with a solution of skim milk powder dust, indicating the presence of antigenic determinants of whey and casein milk proteins in it and a real ability to form cross-allergic reactions in the body of workers to dust from all dry milk processing products containing these proteins.

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COCONUT MILK MODULATE THE ANTIGENICITY OF ALPHA-LACTALBUMIN IN BALB/C MICE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i3.15753 ◽

2017 ◽

Vol 9 (3) ◽

pp. 290 ◽

Cited By ~ 1

Author(s):

Yamina Benaissa ◽

Samia Addou ◽

Wafaa Dib ◽

Omar Kheroua ◽

Djamel Saidi

Keyword(s):

Histological Study ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Coconut Milk ◽

Cow’S Milk ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Milk Formula ◽

Cow's Milk

Objective: The aim of this work was to study the biochemical characteristics of coconut milk and its antigenic effect on the Balb/c mice immunized with α-lactalbumin protein, as well as its consequences on the structure of the intestinal epithelium.Methods: To achieve the objective of the study, an electrophoresis was realised on a polyacrylamide gel to determine various proteins contained in coconut milk. In addition, Lowry’s method was used to determine the amount of proteins in the formula. The antigenicity of coconut milk in sera was also studied using the Enzyme-Linked Immunosorbent Assay (ELISA) method. For the histological study, 21 w-old mice Balb/c were used and distributed in three groups of 7 mice each. Group 1, received a standard feed with no treatment (Negative control), group 2 and 3 received respectively a standard feed (Positive control) and coconut milk for a period of 28 d after being immunized with α- lactalbumin.Results: Analysis of the data revealed that the rate of proteins of cow’s milk is higher than that of the coconut milk ( p0.01). However, after carrying out the electrophoresis analysis, the coconut milk showed the absence of intact proteins. The anti α-Lactalbumin IgG titers significantly increased in positive control groups that received coconut milk (p<0.0001). Moreover, there was an increase of the intestinal villi height of mice fed with coconut milk, in the structure level of their intestinal epithelium compared to the negative control group.Conclusion: The findings of the study provide the evidence that coconut milk is a possible alternative to the cow’s milk formula in case of allergy.

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Analysis of Allergen-Specific T Cell and IgE Reactivity to Different Preparations of Cow’s Milk-Containing Food Extracts

Cells ◽

10.3390/cells8070667 ◽

2019 ◽

Vol 8 (7) ◽

pp. 667 ◽

Cited By ~ 2

Author(s):

Meng Chen ◽

Aaron Sutherland ◽

Giovanni Birrueta ◽

Susan Laubach ◽

Stephanie Leonard ◽

...

Keyword(s):

T Cell ◽

Skin Prick Test ◽

Food Allergies ◽

Cow’S Milk ◽

Cell Reactivity ◽

Prick Test ◽

Cow's Milk ◽

Ige Reactivity ◽

Significant Difference ◽

T Cell Reactivity

Background: cow’s milk allergy (CM) is among the most common food allergies in young children and is often outgrown by adulthood. Prior to developing a tolerance to CM, a majority of CM-allergic children may tolerate extensively-heated CM. This study aims to characterize the IgE- and T cell-reactivity to unheated CM and the progressively more heated CM-containing foods. Methods: CM-containing food extracts from muffin, baked cheese, custard and raw, pasteurized CM commercial extract were tested for skin prick test reactivity, IgE binding and T cell reactivity as assessed by IL-5 and IFNγ production. Results: the skin prick test (SPT) reactivity was significantly decreased to muffin extract compared to raw, pasteurized CM. Both IgE- and T-cell reactivity were readily detectable against food extracts from all forms of CM. Western blot analysis of IgE reactivity revealed variability between extracts that was protein-specific. T cell-reactivity was detected against all four extracts with no significant difference in IL-5 or IFNγ production between them. Conclusion: our data indicate that despite reduced clinical reactivity, extracts from heated CM-containing foods retain immunogenicity when tested in vitro, particularly at the T cell level.

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ЕФЕКТИВНІСТЬ ЕЛІМІНАЦІЙНОЇ ДІЄТИ У ДІТЕЙ ГРУДНОГО ВІКУ З ГАСТРОІНТЕСТИНАЛЬНИМИ ПРОЯВАМИ ХАРЧОВОЇ АЛЕРГІЇ

INTERNATIONAL ACADEMY JOURNAL Web of Scholar ◽

10.31435/rsglobal_wos/31052019/6500 ◽

2019 ◽

pp. 16-21

Author(s):

Гайдучик Г. А.

Keyword(s):

Milk Proteins ◽

Diet Therapy ◽

Elimination Diet ◽

Cow’S Milk ◽

Complete Regression ◽

Cationic Protein ◽

Cow's Milk ◽

Skin Symptoms ◽

Gastrointestinal Food Allergy ◽

Cow’S Milk Proteins

The purpose of the work was to evaluate the effectiveness of the elimination diet in infants with gastrointestinal manifestations of food allergy.Patients and methods. 84 children aged 2 months to 1 year were examined with gastrointestinal food allergy to cow's milk proteins. All children were prescribed an elimination diet with the exclusion of cow's milk proteins. An assessment was made of the regression of gastrointestinal symptoms on day 3, 7 and 14 of diet therapy and the determination of the concentration of eosinophilic cationic protein in serum. The general-clinical, immunological, allergological and statistical methods of research were used.Results. The purpose of the elimination diet with the exclusion of cow's milk proteins for nursing mothers and the use of mixtures with extensive hydrolysis of protein in the case of artificial feeding leads to a complete regression of gastrointestinal and skin symptoms in all examined children on the 14th day of diet therapy, which is further confirmed by the normalization of the concentration of eosinophilic cationic serum protein in diet therapy. Conclusions. Elimination of cow's milk proteins in infants with gastrointestinal food allergy results in complete regression of gastrointestinal and skin symptoms on day 14 of diet therapy, and determination of the concentration of eosinophilic cationic protein in serum can be used as an additional criterion for the effectiveness of diet therapy.

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Study of Proportion of Milk with Lesser Yam Filtrate and Starter Concentration for Producing Synbiotic Yoghurt

International Journal of Eco-Innovation in Science and Engineering ◽

10.33005/ijeise.v2i02.42 ◽

2021 ◽

Vol 2 (02) ◽

pp. 20-25

Author(s):

Rosida Rosida ◽

Sintha Soraya Santi ◽

Rohman

Keyword(s):

Preference Score ◽

Cow’S Milk ◽

Soluble Fiber ◽

Total Dietary Fiber ◽

Cow's Milk ◽

Randomized Design ◽

Factor Ii ◽

Significant Difference ◽

Two Factors ◽

Completely Randomized Design

This study aimed to determine the effect of the proportion of cow's milk with lesser yam filtrate and starter concentration on the characteristics of synbiotic yoghurt. The research used a factorial completely randomized design (CRD) with two factors, factor I was the proportion of cow's milk with lesser yam filtrate (100:0; 60:40; 50:50; 40:60; 0:100) and factor II was the starter cocentration (3%, 5%, 7%). The data obtained were analyzed using analysis of variant (ANOVA) and if there was a significant difference between treatments, then continued with Duncan's test at the 5% level. Synbiotic yoghurt from proportion of cow's milk with lesser yam filtrate (50:50) and 5% starter concentration was the best treatment with total Lactic Acid Bacteria of 7.23 log CFU/ml; pH 4.20; total dietary fiber 3.05%, soluble fiber content 1.3%, inulin content 1.2%, fat content 0.41% and soluble protein content 2.66% with an average texture preference score of 6.13 (like much); taste core 6.02 (like much) and smell score 5.20 (like).

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Oral immunotherapy for IgE-mediated cow’s milk allergy in children: a systematic review and meta-analysis

10.22541/au.162786459.90340572/v1 ◽

2021 ◽

Author(s):

Lujing Tang ◽

Yu Yu ◽

Xiangyuan Pu ◽

Jie Chen

Keyword(s):

Meta Analysis ◽

Oral Immunotherapy ◽

Cow’S Milk Allergy ◽

Cow’S Milk ◽

Control Group ◽

Milk Allergy ◽

Cow's Milk ◽

Cow's Milk Allergy ◽

Statistical Heterogeneity ◽

Ige Mediated

Background: Cow’s milk allergy(CMA) is the most common allergy in infants that decreases the quality of life of patients and their families. Standard treatment for CMA is the strict avoidance of milk, new treatment strategies such as oral immunotherapy (OIT) have been sought for patients with CMA . We aimed to assess the clinical efficacy and safety of OIT in the treatment of children with IgE-mediated cow’s milk allergy (IMCMA). Methods: We searched all randomized controlled trials (RCTs) in which OIT is used to treat children with IMCMA from 5 international electronic databases. We estimated a pooled relative ratio (RR) for each outcome using a Mantel-Haenzel fixed-effect model if statistical heterogeneity was low. Results: Eleven studies were chosen for meta-analysis, including a total of 469 children (242 OIT, 227 control). 176 patients (72.7%) in the OIT were desensitized compared to 49 patients in the control group (RR 7.35, 95%CI 2.82-19.13, p<0.0001). The desensitization effect of OIT was particularly significant in children over 3 years old (RR 18.05, 95%CI 6.48-50.26, p<0.00001). Although adverse effects were common, they usually involved mild reactions, but epinephrine use was more common in the OIT group (RR 7.69, 95%CI 2.16-27.33, p<0.002). Conclusion: OIT can lead to desensitization in the majority of individuals with IMCMA, especially in patients over 3-years old. A major problem of OIT is the frequency of adverse events, although most are mild. OIT may be an alternative treatment in the future.

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