Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

2019 ◽  
Vol 8 (3) ◽  
pp. 76-82
Author(s):  
K. Zerhouni ◽  
B. Abbouni ◽  
K. Kanoun ◽  
K. Larbi Daouadji ◽  
A. Tifrit ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Daily Lives ◽  
North West ◽  
Degrading Bacteria ◽  
Solid Culture Medium ◽  
Chemical Cycling

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

Isolation and identification of low-density polyethylene degrading novel bacterial strains

2021 ◽  
Author(s):  
Habibullah Nadeem ◽  
Khush Bakhat Alia ◽  
Faizan Muneer ◽  
Ijaz Rasul ◽  
Muhammad Hussnain Siddique ◽  
...  
Keyword(s):  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Isolation And Identification

Cellulase enzyme catalyst producing bacterial strains from vermicompost and its application in low-density polyethylene degradation

Chemosphere ◽  
2021 ◽  
pp. 132552
Author(s):  
Veena Gayathri Krishnaswamy ◽  
Rajalakshmi Sridharan ◽  
P. Senthil Kumar ◽  
Mariyam Jaffer Fathima
Keyword(s):  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Cellulase Enzyme ◽  
Enzyme Catalyst

scholarly journals Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Christabel Ndahebwa Muhonja ◽  
Gabriel Magoma ◽  
Mabel Imbuga ◽  
Huxley Mae Makonde
Keyword(s):  
Bacillus Cereus ◽  
Aspergillus Oryzae ◽  
Bacterial Degradation ◽  
Low Density ◽  
Optimum Growth ◽  
Alkane Hydroxylase ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Bacteria And Fungi

This study aimed at molecular and biochemical characterization of low-density polyethene (LDPE) degrading fungi and bacteria from Dandora dumpsite, Nairobi. Twenty bacterial and 10 fungal isolates were identified using 16S rDNA and 18S rDNA sequences for bacteria and fungi, respectively. The highest fungal degradation was attributed to Aspergillus oryzae strain A5,1 while the highest bacterial degradation was attributed to Bacillus cereus strain A5,a and Brevibacillus borstelensis strain B2,2, respectively. Isolates were screened for their ability to produce extracellular laccase and esterase; Aspergillus fumigatus strain B2,2 exhibited the highest presence of laccase (15.67 mm) while Aspergillus oryzae strain A5,1 exhibited the highest presence of esterase (14.33 mm). Alkane hydroxylase-encoding genes were screened for using primer AlkB 1 which amplified the fragment of size 870 bp. Four bacterial samples were positive for the gene. Optimum growth temperature of the fungal isolates was 30°C. The possession of laccase, esterase, and alkane hydroxylase activities is suggested as key molecular basis for LDPE degrading capacity. Knowledge of optimum growth conditions will serve to better utilize microbes in the bioremediation of LDPE. The application of Aspergillus oryzae strain A5,1 and Bacillus cereus strain A5,a in polyethene degradation is a promising option in this kind of bioremediation as they exhibited significantly high levels of biodegradation. Further investigation of more alkane degrading genes in biodegrading microbes will inform the choice of the right microbial consortia for bioaugmentation strategies.

scholarly journals Ability of Trichoderma hamatum Isolated from Plastics-Polluted Environments to Attack Petroleum-Based, Synthetic Polymer Films

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 467
Author(s):  
Kateřina Malachová ◽  
Čeněk Novotný ◽  
Grażyna Adamus ◽  
Nadia Lotti ◽  
Zuzana Rybková ◽  
...  
Keyword(s):  
Functional Groups ◽  
Polymer Surface ◽  
Microbial Consortia ◽  
Low Density Polyethylene ◽  
High Temperature Treatment ◽  
Rrna Gene ◽  
Its Sequencing ◽  
Low Density ◽  
Bacterial Strains ◽  
Trichoderma Hamatum

Microorganisms colonizing plastic waste material collected in composting-, landfill-, and anaerobic digestion plants were isolated to obtain novel strains maximally adapted to the degradation of plastics due to long-term contact with plastic polymers. Twenty-six bacterial strains were isolated and identified by the 16 S rRNA method, and eighteen strains of yeasts and fungi using 18 S rRNA and the internal transcribed spacer ITS sequencing of the 18 S rRNA gene. In selected strains, the ability to degrade linear low-density polyethylene (LLDPE), low-density polyethylene (LDPE), polystyrene (PS), and polyvinyl chloride (PVC) was tested in aerobic liquid-medium cultures. An oxidative, two-step pretreatment of LLDPE and LDPE using γ- or UV-irradiation followed by a high-temperature treatment was carried out, and the pretreated plastics were also included in the degradation experiments. The respective weight losses after biodegradation by Trichoderma hamatum were: virgin and γ/T90-pretreated LLDPE (2.2 ± 1.2 and 3.9 ± 0.5%), virgin and UV/T60-pretreated LDPE (0.5 ± 0.4 and 1.3 ± 0.4%), and virgin PS (0.9 ± 0.4%). The Fourier transform infrared spectroscopy (FTIR) analysis showed that during the treatment of pretreated LLDPE, T. hamatum attacked low molecular weight LLDPE oligomers, reducing the functional groups (carbonyl C = O), which was paralleled by a slight increase of the molar mass of pretreated LLDPE and a decrease of the dispersity index, as demonstrated by gel permeation chromatography (GPC). Thermogravimetric analysis (TGA) highlighted the formation of functional groups on LLDPE due to polymer pretreatment that favored fungal attack at the polymer surface. The results provide insight into microbial consortia that spontaneously colonize the surface of plastics in various environments and their capability to attack plastic polymers.

scholarly journals Eksplorasi Bakteri Indigen Pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ]

2019 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Prayogo, Boedi Setya Rahardja, Abdul Manan
Keyword(s):  
Organic Matter ◽  
Organic Materials ◽  
Pseudomonas Stutzeri ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Biological Filtration ◽  
Degrading Bacteria ◽  
Recirculation System ◽  
Pseudomonas Pseudomallei

Abstract The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.

Isolation and identification of low-density polyethylene (LDPE) biodegrading bacteria from waste landfill in Yazd

2018 ◽  
Vol 76 (2) ◽  
pp. 236-250 ◽  
Author(s):  
Asieh Nourollahi ◽  
Samaneh Sedighi-Khavidak ◽  
Mehdi Mokhtari ◽  
Gilda Eslami ◽  
Mahbubeh Shiranian
Keyword(s):  
Low Density Polyethylene ◽  
Low Density ◽  
Isolation And Identification ◽  
Waste Landfill

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

2021 ◽  
Vol 16 (3) ◽  
pp. 130-138
Author(s):  
Fazilah Arifin ◽  
Rozilawati Mohamed Razali ◽  
Vigneswari Sevakumaran
Keyword(s):  
16S Rdna ◽  
Anthropogenic Activities ◽  
Morphological Characteristics ◽  
Gravimetric Method ◽  
Diesel Oil ◽  
Plate Count ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Diesel Degradation

Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.

scholarly journals Screening, Isolation and Biochemical Characterization of Laccase Producing Bacteria from Contaminated Sites

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Siva Jyothi J. ◽  
Kishore Kumar K. ◽  
Hema Latha E.
Keyword(s):  
Biochemical Characterization ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Laccase Production ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Leading Role ◽  
Degrading Bacteria ◽  
Nutrient Agar Medium ◽  
Guntur District

Screening and isolation of Laccase producing bacteria from Guntur District soil was carried out to assess the diversity of Lignocellulose degrading bacteria. Isolation and identification of environmental friendly bacteria for lignin degradation becomes an essential one, because all the researchers are mainly concentrating on fungal strains. However, bacteria seem to play a leading role in decomposing lignin. For isolation of Laccase producing bacteria nutrient agar medium containing guaiacol was used. Total nine bacterial strains were isolated from soil samples collected from different regions of Guntur district. Preliminary screening of bacterial strains was carried out on guaiacol containing nutrient agar medium for laccase production. Formation of green colour using ABTS (2,2'- azinobis(3-ethylbenzthiazoline-6-sulfonate) confirms the capability of laccase production by the bacterial strains. Nine bacterial strains showed positive results. High laccase producing bacterial isolates were examined for morphological and biochemical characteristics according to Bergey’s Manual of Systematic Bacteriology. The predominant isolates were identified as Bacillus and Enterobacter species.

scholarly journals Short Communication: Diversity of Chlorpyrifos-degrading bacteria isolated from shallow aquifer of East Java Coastal Settlements, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
BASKORO ROCHADDI ◽  
Muhammad Zainuri ◽  
AGUS SABDONO
Keyword(s):  
Bacillus Cereus ◽  
Gene Sequence ◽  
Short Communication ◽  
Shallow Aquifer ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Kinetic Growth ◽  
Degrading Bacteria ◽  
The 16S Rrna Gene ◽  
Coastal Settlement

Abstract. Rochaddi B, Zainuri M, Sabdono A. 2019. Short Communication: Diversity of Chlorpyrifos-degrading bacteria isolated from shallow aquifer of East Java Coastal Settlements, Indonesia. Biodiversitas 20: 3662-3666. Wastes from inhabited regions, factories and maricultural practices through movement and penetration enter to groundwaters. A study was undertaken to determine the diversity of Chlorpyrifos-degrading bacteria isolated from a shallow aquifer of East Java coastal settlements. Fifteen of 116 bacterial strains isolated from shallow aquifer samples of 12 household wells were selected due to their capability of degrading Chlorpyrifos herbicide. These isolates were different in the ability of Chlorpyrifos degradation. They utilize Chlorpyrifos as the source of carbon and energy for their growth. Their initial degradation at 50 mg L−1 concentration within the first 4 days ranged between 26.81 and 70.12 %. The 16S rRNA gene sequence analyses indicated that the majority of the isolates belonged to members of Bacillus genera. These bacterial strains were Bacillus cereus (seven strains) and Bacillus paramycoides (four strains). Besides, three strains were identified as Bacillus subtilis and one strain as Bacillus thuringiensis. Bacillus cereus strain LCA1.1 was selected for further study on kinetic growth and Chlorpyrifos utilization. These bacterial strains have a great potential utility for the bioremediation of shallow aquifer of coastal settlement contaminated with Chlorpyrifos pesticides.

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