scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

2021 ◽  
Vol 16 (3) ◽  
pp. 130-138
Author(s):  
Fazilah Arifin ◽  
Rozilawati Mohamed Razali ◽  
Vigneswari Sevakumaran
Keyword(s):  
16S Rdna ◽  
Anthropogenic Activities ◽  
Morphological Characteristics ◽  
Gravimetric Method ◽  
Diesel Oil ◽  
Plate Count ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Degrading Bacteria ◽  
Diesel Degradation

Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.

scholarly journals Isolation and Identification of Pesticides Degrading Bacteria from Farmland Soil

2019 ◽  
Vol 35 (2) ◽  
pp. 90-94
Author(s):  
Md Atikur Rahman ◽  
ASM Shamsul Arefin ◽  
Otun Saha ◽  
Md Mizanur Rahaman
Keyword(s):  
16S Rdna ◽  
Restriction Analysis ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Rdna Sequencing ◽  
Degrading Bacteria ◽  
Farmland Soil ◽  
Wide Range ◽  
Dna Restriction

Pesticides are recognized to be the threat to the environment and associated with a wide range of serious diseases including respiratory diseases, cancer and even birth defects. In this study, six-different bacterial strains capable of degrading Carbofuran, Emamectin Benzoate and Thiamethoxam were isolated from eight different soil samples. The isolates were characterized by using different conventional and molecular methods. The strains were identified molecularly into different genotypes using amplified ribosomal DNA restriction analysis (ARDRA) and partial sequencing of 16S rDNA. The ARDRA pattern clustered them into 3 groups. Among the isolates three were identified as Achromobacter spp. and one as Diaphorobacter sp. by biochemical tests. It was further confirmed by the partial 16S rDNA sequencing. The two identified potential bacteria can be used for biodegradation of different pesticides which can have a significant environmental impact in soil farm. Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 90-94

scholarly journals Isolation and Identification of Hydrocarbon-Degrading Bacteria that Tolerant to Saponin of Sapindus Rarak Plant

2019 ◽  
Vol 4 (1) ◽  
pp. 79-88
Author(s):  
Evi Octaviany ◽  
Suharjono Suharjono ◽  
Irfan Mustafa
Keyword(s):  
Crude Oil ◽  
16S Rdna ◽  
Bacterial Isolates ◽  
High Concentration ◽  
Isolation And Identification ◽  
Rdna Sequences ◽  
Degrading Bacteria ◽  
16S Rdna Sequences ◽  
Petroleum Contaminated Soil ◽  
Cell Densities

A commercial saponin as biosurfactant can reduce the surface tension of water and increase of hydrocarbon degradation. However, this saponin can be toxic to some hydrocarbonoclastic bac-teria. This study aimed to obtain bacterial isolates that were tolerant and incapable to degrade saponin, and to identify them based on 16S rDNA sequence. Bacteria were isolated from petroleum contaminated soil in Wonocolo Village, Bojonegoro Regency, East Java, Indonesia. The soil samples were acclimated using Bushnell-Haas (BH) broth with 0.5% crude oil at room temperature for 3 weeks. The culture was spread onto BH agar incubated at 30°C for 7 days. The first screened, isolates were grown in nutrient broth with addition of sap-onin 0%, 8%, and 12% (v/v) then incubated at 30°C for three days. The bacterial cell density was measured using a spectrophotometer. Second screened, the isolates were grown on BH broth with addition of 0.5% saponin as a sole carbon source, and their cell densities were measured. The selected isolates were identified based on 16S rDNA sequences. Among 34 bacterial isolates, nine isolates were tol-erant to 12% saponin. Three bacterial isolates IHT1.3, IHT1.5, and IHT3.24 tolerant to high concentration of saponin and did not use this substance as growth nutrition. The IHT1.3, IHT1.5, and IHT3.24 isolates were identified as Ochrobactrum pseudogrignonense (99% similarity), Pseudomonas mendocina (99%), and Ochrobactrum pi-tuitosum; (97%), respectively. Those three selected isolates are good candidates as hydrocarbon-degrading bacteria to bioremediation of soil contaminated crude oil. However, the combined activity of bacteria and saponin to degrade hydrocarbon needs further study. 

scholarly journals Eksplorasi Bakteri Indigen Pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ]

2019 ◽  
Vol 4 (2) ◽  
pp. 193
Author(s):  
Prayogo, Boedi Setya Rahardja, Abdul Manan
Keyword(s):  
Organic Matter ◽  
Organic Materials ◽  
Pseudomonas Stutzeri ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Biological Filtration ◽  
Degrading Bacteria ◽  
Recirculation System ◽  
Pseudomonas Pseudomallei

Abstract The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.

Isolation and identification of low density polythene-degrading bacteria from soil of North West of Algeria

2019 ◽  
Vol 8 (3) ◽  
pp. 76-82
Author(s):  
K. Zerhouni ◽  
B. Abbouni ◽  
K. Kanoun ◽  
K. Larbi Daouadji ◽  
A. Tifrit ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Daily Lives ◽  
North West ◽  
Degrading Bacteria ◽  
Solid Culture Medium ◽  
Chemical Cycling

Plas c bags (Low Density Polyethylene (LDPE) belong to the polymers, which plays a very important role in our daily lives by their diversi ed applica on. However, the accumula on of the plas c bags in the environment cons - tutes a serious problem and a real source for visual nuisance, pollu on of soil and marine environments. Furthermore, their biodegradation was the safest method of breakdown that possibly leaves behind less toxic residues and showed poten al of bio-geo chemical cycling of the substrate. The aim of the present work was the characterization of the isolated bacterial strains from a municipal land ll area of Tlemcen, North West Algeria, which were implicated by the biodegrada on ability of the Low Density Polyethylene. The degradation of the Low Density Polyethylene was inves gated by studying the bacterial growth of the isolated, inoculated on a solid culture medium, which was composed of LDPE as the sole carbon source with and with- out a nitrogen source and the selec on was based by the determination of the produced diameter of hydrolysis clear zone on the surface. Furthermore, the isolated, selected degrading Low Density Polyethylene bacterial ML002 has been iden ed by the study of their morphological, biochemical charac- teris cs and the ampli ca on of the fragment, coding the region of ARN 16S. The use of the API system indicated their belonging to the genus Bacillus Cereus, which has reduced the weight of LDPE by 0.26, 1.28, 1.53% a er 30, 90, 120 days respec vely. Furthermore, the amplified of the fragment, coding the region of ARN 16S by the isolated, selected bacterial ML002 indicated a similarity of 99.394% with Bacillus wiedmannii and Bacillus proteolyticus and 99.293% homology with Bacillus toyonensis, Bacillus cereus and Bacillus thuringiensis.

scholarly journals Screening, Isolation and Biochemical Characterization of Laccase Producing Bacteria from Contaminated Sites

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Siva Jyothi J. ◽  
Kishore Kumar K. ◽  
Hema Latha E.
Keyword(s):  
Biochemical Characterization ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Laccase Production ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Leading Role ◽  
Degrading Bacteria ◽  
Nutrient Agar Medium ◽  
Guntur District

Screening and isolation of Laccase producing bacteria from Guntur District soil was carried out to assess the diversity of Lignocellulose degrading bacteria. Isolation and identification of environmental friendly bacteria for lignin degradation becomes an essential one, because all the researchers are mainly concentrating on fungal strains. However, bacteria seem to play a leading role in decomposing lignin. For isolation of Laccase producing bacteria nutrient agar medium containing guaiacol was used. Total nine bacterial strains were isolated from soil samples collected from different regions of Guntur district. Preliminary screening of bacterial strains was carried out on guaiacol containing nutrient agar medium for laccase production. Formation of green colour using ABTS (2,2'- azinobis(3-ethylbenzthiazoline-6-sulfonate) confirms the capability of laccase production by the bacterial strains. Nine bacterial strains showed positive results. High laccase producing bacterial isolates were examined for morphological and biochemical characteristics according to Bergey’s Manual of Systematic Bacteriology. The predominant isolates were identified as Bacillus and Enterobacter species.

Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. from Antarctica

2020 ◽  
Vol 32 (5) ◽  
pp. 341-353 ◽  
Author(s):  
Mansur Abdulrasheed ◽  
Nur Nadhirah Zakaria ◽  
Ahmad Fareez Ahmad Roslee ◽  
Mohd Yunus Shukor ◽  
Azham Zulkharnain ◽  
...  
Keyword(s):  
Oil Spills ◽  
Growth Conditions ◽  
Diesel Oil ◽  
Bacterial Strains ◽  
Antarctic Soils ◽  
Cold Tolerant ◽  
Antarctic Treaty ◽  
Degradation Activity ◽  
The Antarctic ◽  
Diesel Degradation

AbstractBioremediation has been proposed as a means of dealing with oil spills on the continent. However, the introduction of non-native organisms, including microbes, even for this purpose would appear to breach the terms of the Environmental Protocol to the Antarctic Treaty. This study therefore aimed to optimize the growth conditions and diesel degradation activity of the Antarctic native bacteria Arthrobacter spp. strains AQ5-05 and AQ5-06 through the application of a one-factor-at-a-time (OFAT) approach. Both strains were psychrotolerant, with the optimum temperature supporting diesel degradation being 10–15°C. Both strains were also screened for biosurfactant production and biofilm formation. Their diesel degradation potential was assessed using Bushnell–Haas medium supplemented with 0.5% (v/v) diesel as the sole carbon source and determined using both gravimetric and gas chromatography and mass spectrophotometry analysis. Strain AQ5-06 achieved 37.5% diesel degradation, while strain AQ5-05 achieved 34.5% diesel degradation. Both strains produced biosurfactants and showed high biofilm adherence. Strains AQ5-05 and AQ5-06 showed high cellular hydrophobicity rates of 73.0% and 81.5%, respectively, in hexadecane, with somewhat lower values of 60.5% and 70.5%, respectively, in tetrahexadecane. Optimized conditions identified via OFAT increased diesel degradation to 41.0% and 47.5% for strains AQ5-05 and AQ5-06, respectively. Both strains also demonstrated the ability to degrade diesel in the presence of heavy metal co-pollutants. This study therefore confirms the potential use of these cold-tolerant bacterial strains in the biodegradation of diesel-polluted Antarctic soils at low environmental temperatures.

scholarly journals Bakteri Pendegradasi Solar Dari Sedimen Perairan Dalam Skala Laboratorium (In Vitro)

2020 ◽  
Vol 9 (4) ◽  
pp. 453-463
Author(s):  
Bagus Enggal Prakoso ◽  
Widianingsih Widianingsih ◽  
Sunaryo Sunaryo
Keyword(s):  
Gravimetric Method ◽  
Diesel Oil ◽  
Vibrio Alginolyticus ◽  
Plate Count ◽  
Anthropogenic Activity ◽  
Molecular Testing ◽  
Bacterial Isolates ◽  
Standard Plate Count ◽  
Standard Plate ◽  
Initial Selection

 ABSTRAK: Pelabuhan Tanjung Mas adalah sebuah pelabuhan yang mengalami berbagai aktivitas seperti bongkar muat, aktivitas pelayaran, kapal bersandar dan lain-lain. Aktivitas antropogenik yang ada di area pelabuhan umumnya menyebabkan banyaknya jumlah minyak solar yang tumpah ke laut dan kemudian terakumulasi ke dalam sedimen dalam jangka waktu yang panjang. Jika hal ini terus terjadi maka akan mengganggu kehidupan biota demersal dan ekosistem di Pelabuhan Tanjung Mas. Oleh karena itu, perlu dilakukan upaya biodegradasi minyak solar menggunakan bakteri  yang ramah lingkungan dan tepat. Tujuan penelitian ini dilakukan yaitu mendapatkan isolat bakteri sedimen yang mampu mendegradasi minyak solar dan mengetahui tingkat kemampuan isolat bakteri untuk mendegradasi minyak solar. Penelitian ini dilaksanakan pada bulan Juli sampai dengan Oktober 2019 dengan metode purpossive sampling, metode pour plate, metode gravimetri dan Standard Plate Count. Bakteri isolat yang didapat dari hasil isolasi sedimen kemudian diseleksi kembali menggunakan media SMSSe dan prosentase minyak solar 2%. Pada seleksi awal dengan prosentase minyak solar 2% didapatkan 2 isolat bakteri yang mampu tumbah baik pada media SMSSe dan 2% prosentase minyak solar, yaitu bakteri dengan kode isolat ISL 2 dan ISL 3. Kedua isolat bakteri hasil seleksi awal kemudian diidentifikasi dengan uji molekuler. Hasil dari uji molekuler menyatakan bahwa kedua isolat bakteri dengan kode ISL 2 dan ISL 3 berasal dari spesies yang sama, yaitu Vibrio alginolyticus. Isolat bakteri Vibrio alginlyticus yang didapat kemudian diuji daya degradasinya dengan menggunakan 2 prosentase minyak solar yang berbeda, yaitu prosentase 5% dan 10% dengan 2 pengulangan. Pada hasil uji degradasi menyatakan bahwa bakteri Vibrio alginolyticus dapat mendegradasi minyak solar yang ditambahkan pada media SMSSe. Bakteri Vibrio alginolyticus hasil isolasi mampu mendegradasi minyak solar hingga 0,8085 mL (80,85%) pada prosentase awal 10% minyak solar (1 mL) dengan periode 14 hari (H14). ABSTRACT: Tanjung Mas Port is a port that experiences a variety of activities such as loading and unloading, shipping activities, leaning ships, and others. Generally, anthropogenic activity in the port area causes large amounts of diesel oil that spills into the sea and then accumulates into the sediment over a long period. If this continues, it will disrupt the lives of demersal biota and the ecosystem in Tanjung Mas Harbor. Therefore, it is necessary to do biodegradation of diesel oil using environmentally friendly and appropriate bacteria. The purpose of this study was to obtain bacterial sediment isolates that can degrade diesel oil and determine the ability of bacterial isolates to degrade diesel oil. This research was conducted from July to October 2019 using the purposive sampling method, pour plate method, gravimetric method, and Standard Plate Count. Bacteria isolates obtained from the results of sediment isolation were then reselected using SMSSe media and a percentage of 2% diesel oil. In the initial selection with a percentage of 2% diesel oil, 2 bacterial isolates were able to grow well on SMSSe media and 2% percentage of diesel oil, namely bacteria with isolate code ISL 2 and ISL 3. The two bacterial isolates from the initial selection were identified by molecular testing. The results of the molecular test stated that the two bacterial isolates with the code ISL 2 and ISL 3 came from the same species, namely Vibrio alginolyticus. The Vibrio alginolyticus bacterial isolate obtained was then tested for its degradation power using 2 different percentages of diesel oil, namely 5% and 10% with 2 repetitions. The results of the degradation test stated that the Vibrio alginolyticus bacteria could degrade the diesel oil added to the SMSSe medium. The isolated Vibrio alginolyticus bacteria can degrade diesel oil up to 0.8085 mL (80.85%) at the initial percentage of 10% diesel oil (1 mL) with a period of 14 days (H14).

scholarly journals Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials

2021 ◽  
Vol 9 (2) ◽  
pp. 7-12
Author(s):  
Abdulrahman Abdulhamid Arabo ◽  
Raji Arabi Bamanga ◽  
Mujiburrahman Fadilu ◽  
Musa Abubakar ◽  
Fatima Abdullahi Shehu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Incubation Time ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Screening Methods ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Degrading Bacteria

This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.

scholarly journals Cyperus laevigatus L. Enhances Diesel Oil Remediation in Synergism with Bacterial Inoculation in Floating Treatment Wetlands

2020 ◽  
Vol 12 (6) ◽  
pp. 2353 ◽  
Author(s):  
Muhammad Fahid ◽  
Shafaqat Ali ◽  
Ghulam Shabir ◽  
Sajid Rashid Ahmad ◽  
Tahira Yasmeen ◽  
...  
Keyword(s):  
Bacterial Consortium ◽  
Pilot Scale ◽  
Diesel Oil ◽  
Treatment Wetlands ◽  
Contaminated Water ◽  
Bacterial Strains ◽  
Joint Application ◽  
Degrading Bacteria ◽  
Acinetobacter Sp ◽  
Floating Treatment Wetlands

Diesel oil is considered a very hazardous fuel due to its adverse effect on the aquatic ecosystem, so its remediation has become the focus of much attention. Taking this into consideration, the current study was conducted to explore the synergistic applications of both plant and bacteria for cleaning up of diesel oil contaminated water. We examined that the application of floating treatment wetlands (FTWs) is an economical and superlative choice for the treatment of diesel oil contaminated water. In this study, a pilot scale floating treatment wetlands system having diesel oil contaminated water (1% w/v), was adopted using Cyperus laevigatus L and a mixture of hydrocarbons degrading bacterial strains; viz., Acinetobacter sp.61KJ620863, Bacillus megaterium 65 KF478214, and Acinetobacter sp.82 KF478231. It was observed that consortium of hydrocarbons degrading bacteria improved the remediation of diesel oil in combination with Cyperus laevigatus L. Moreover, the performance of the FTWs was enhanced by colonization of bacterial strains in the root and shoot of Cyperus laevigatus L. Independently, the bacterial consortium and Cyperus laevigatus L exhibited 37.46% and 56.57% reduction in diesel oil, respectively, while 73.48% reduction in hydrocarbons was exhibited by the joint application of both plant and bacteria in FTWs. Furthermore, microbial inoculation improved the fresh biomass (11.62%), dry biomass (33.33%), and height (18.05%) of plants. Fish toxicity assay evaluated the effectiveness of FTWs by showing the extent of improvement in the water quality to a level that became safe for living organisms. The study therefore concluded that Cyperus laevigatus L augmented with hydrocarbons degrading bacterial consortium exhibited a remarkable ability to decontaminate the diesel oil from water and could enhance the FTWs performance.

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