Determination of oleanolic acid and ursolic acid in Oldenlandia diffusa and its substitute using high performance liquid chromatography

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Z. Liang ◽  
Z. Jiang ◽  
D.W. Fong ◽  
Z. Zhao
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Oldenlandia Diffusa

scholarly journals Simultaneous Analysis of Ursolic Acid and Oleanolic Acid in Guava Leaves Using QuEChERS-Based Extraction Followed by High-Performance Liquid Chromatography

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Chang Xu ◽  
Yiyi Liao ◽  
Chunyan Fang ◽  
Makoto Tsunoda ◽  
Yingxia Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Correlation Coefficients ◽  
Guava Leaves ◽  
Guava Leaf

In this paper, a novel method of QuEChERS-based extraction coupled with high-performance liquid chromatography has been developed for the simultaneous determination of ursolic acid (UA) and oleanolic acid (OA) in guava leaves. The QuEChERS-based extraction parameters, including the amount of added salt, vortex-assisted extraction time, and absorbent amount, and the chromatographic conditions were investigated for the analysis of UA and OA in guava leaves. Under the optimized conditions, the method showed good linearity over a range of 1–320 μg mL−1, with correlation coefficients above 0.999. The limits of detection of UA and OA were 0.18 and 0.36 μg mL−1, respectively. The intraday and interday precision were below 1.95 and 2.55%, respectively. The accuracies of the UA and OA determinations ranged from 97.4 to 111.4%. The contents of UA and OA in the guava leaf samples were 2.50 and 0.73 mg g−1, respectively. These results demonstrate that the developed method is applicable to the simultaneous determination of UA and OA in guava leaves.

scholarly journals Determination of the content of ursolic and oleanolic acid in the cuticular wax of fruits of different species of Rosaceae

2019 ◽  
Vol 48 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Michael A. Ludeña-Huaman ◽  
Deborah A. Ramos-Inquiltupa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ursolic Acid ◽  
Oleanolic Acid ◽  
High Performance ◽  
Cuticular Wax ◽  
Bioactive Properties ◽  
Pharmacological Interest ◽  
Rosaceae Family

Ursolic acid (UA) and oleanolic acid (OA) are two widely distributed triterpenes in fruits, especially those belonging to Rosaceae family. These triterpene isomers are of great pharmacological interest due to their multiple bioactive properties. For this reason, the objective of this study was to determine the content of UA and OA extracted from the cuticular wax of five highly edible fruits (quince, loquat, pear, peach and apple) all belonging to the Rosaceae family. The acids were analyzed by high performance liquid chromatography. Both UA and OA are present in all these fruits, however, UA is in greater quantities.

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