Rapid measurement of retinol, retinal, 13-cis-retinoic acid and all-trans-retinoic acid by high performance liquid chromatography

2020 ◽  
Vol 13 (3) ◽  
Author(s):  
V. Tatum ◽  
C.K. Chow
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retinoic Acid ◽  
High Performance ◽  
Rapid Measurement ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid

scholarly journals Simultaneous Determination of all-trans-Retinoic Acid, β-Carotene, and Vitamin A in Galenic Preparations by Liquid Chromatography

2001 ◽  
Vol 84 (2) ◽  
pp. 354-360
Author(s):  
Vincenzo Pucci ◽  
Francesca Bugamelli ◽  
Roberto Mandrioli ◽  
Maria A Raggi
Keyword(s):  
Liquid Chromatography ◽  
Retinoic Acid ◽  
Vitamin A ◽  
Degradation Products ◽  
Extraction Procedure ◽  
Uv Detector ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Β Carotene

Abstract The concentrations of vitamin A, β-carotene, and all-trans-retinoic acid in oral preparations were determined in a single analysis by a method based on isocratic, reversed-phase liquid chromatography (LC). The LC system consisted of a C18 column, a mobile phase of acetonitrile, dichloromethane, methanol, and water and a UV detector set at 330 nm. The linearity ranges were 25–250 ng/mL for trans-retinoic acid and vitamin A, and 100–1000 ng/mL for β-carotene. This LC method for the determination of retinoids is simple, precise, and accurate. No extraction procedure is required before the chromatographic analysis; only a suitable dilution is necessary. The method proved to be reliable, fast, and economical. Furthermore, this method is indicative of stability, because it allows for the determination of degradation products such as 13-cis-retinoic acid.

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