scholarly journals IMPACT OF PESTICIDE TOLERANT SOIL BACTERIA ON DISEASE CONTROL, PLANT GROWTH PROMOTION AND SYSTEMIC RESISTANCE IN COWPEA

2021 ◽  
Vol 29 (4) ◽  
pp. 430-440
Author(s):  
Anuradha Bandopadhyay ◽  
Tina Roy ◽  
Nirmalendu Das
Keyword(s):  
Plant Growth ◽  
Disease Control ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Control Plant ◽  
Field Evaluation ◽  
Macrophomina Phaseolina ◽  
Systemic Resistance ◽  
Infested Soil

Cowpea, an annual legume, suffers from several disease symptoms caused by Macrophomina phaseolina. Rhizobacteria isolated from pesticide infested soil, identified by blast analysis as Bacillus cereus, Bacillus safensis, Pseudomonas donghuensis and Pseudomonas aeruginosa ascertained tolerant to at least 0.1% pesticides viz. methomyl, imidacloprid and carbendazim. In vitro antagonism against pathogen exhibited maximum by P. aeruginosa 63%. All rhizobacteria were bestowed with attributes responsible for pathogen control and plant growth promotion. Field evaluation resulted highest 75% disease control, enhancement of length, nodule counts, biomass or yield per plant by P. aeruginosa. All rhizobacteria induced systemic resistance in cowpea under challenged inoculation with pathogen by augmenting defensive enzyme production. Highest Phenylalanine Ammonia Lyase activity was expressed in P. aeruginosa treated plants 1.02 μMoles/ml/min, Polyphenol Oxidase by P. donghuensis 1.39 μMoles/ml/min, Chitinase by B. cereus 0.745 μMoles/ml/min and 400 percent relative activity of Peroxidase by P. aeruginosa. The rhizobacteria were prospective for plant disease control, growth promotion and as immunity boosters in pesticide and heavy metal infested toxic environment.

scholarly journals Nematicidal Volatiles from Bacillus atrophaeus GBSC56 Promote Growth and Stimulate Induced Systemic Resistance in Tomato against Meloidogyne incognita

2021 ◽  
Vol 22 (9) ◽  
pp. 5049
Author(s):  
Muhammad Ayaz ◽  
Qurban Ali ◽  
Ayaz Farzand ◽  
Abdur Rashid Khan ◽  
Hongli Ling ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Control Plant ◽  
Nematicidal Activity ◽  
Systemic Resistance ◽  
Tomato Plants ◽  
Nematicidal Volatiles

Bacillus volatiles to control plant nematodes is a topic of great interest among researchers due to its safe and environmentally friendly nature. Bacillus strain GBSC56 isolated from the Tibet region of China showed high nematicidal activity against M. incognita, with 90% mortality as compared with control in a partition plate experiment. Pure volatiles produced by GBSC56 were identified through gas chromatography and mass spectrometry (GC-MS). Among 10 volatile organic compounds (VOCs), 3 volatiles, i.e., dimethyl disulfide (DMDS), methyl isovalerate (MIV), and 2-undecanone (2-UD) showed strong nematicidal activity with a mortality rate of 87%, 83%, and 80%, respectively, against M. incognita. The VOCs induced severe oxidative stress in nematodes, which caused rapid death. Moreover, in the presence of volatiles, the activity of antioxidant enzymes, i.e., SOD, CAT, POD, and APX, was observed to be enhanced in M. incognita-infested roots, which might reduce the adverse effect of oxidative stress-induced after infection. Moreover, genes responsible for plant growth promotion SlCKX1, SlIAA1, and Exp18 showed an upsurge in expression, while AC01 was downregulated in infested plants. Furthermore, the defense-related genes (PR1, PR5, and SlLOX1) in infested tomato plants were upregulated after treatment with MIV and 2-UD. These findings suggest that GBSC56 possesses excellent biocontrol potential against M. incognita. Furthermore, the study provides new insight into the mechanism by which GBSC56 nematicidal volatiles regulate antioxidant enzymes, the key genes involved in plant growth promotion, and the defense mechanism M. incognita-infested tomato plants use to efficiently manage root-knot disease.

EFFICACY OF RHIZOBACTERIA ON PLANT GROWTH PROMOTION AND DISEASE SUPPRESSION IN VITRO

2012 ◽  
pp. 525-532 ◽  
Author(s):  
S. Velivelli ◽  
E. O'Herlihy ◽  
B. Janczura ◽  
B. Doyle Prestwich ◽  
J. Ghyselinck ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression

scholarly journals Perception of Biocontrol Potential of Bacillusinaquosorum KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis

Biology ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 137
Author(s):  
Maedeh Kamali ◽  
Dianjing Guo ◽  
Shahram Naeimi ◽  
Jafar Ahmadi
Keyword(s):  
Chemical Analysis ◽  
Plant Growth ◽  
Fusarium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Genome Mining ◽  
Gene Clusters ◽  
Systemic Resistance ◽  
Plant Colonization ◽  
Biocontrol Potential

Tomato Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against Fol was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as B. inaquosorum based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals Potential for Plant Growth Promotion of Rhizobacteria Associated withSalicorniaGrowing in Tunisian Hypersaline Soils

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Francesca Mapelli ◽  
Ramona Marasco ◽  
Eleonora Rolli ◽  
Marta Barbato ◽  
Hanene Cherif ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Crop Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Growth Promotion ◽  
Arid Ecosystems ◽  
Acetic Acid Production ◽  
Halotolerant Bacteria ◽  
Microbiome Composition

Soil salinity and drought are among the environmental stresses that most severely affect plant growth and production around the world. In this study the rhizospheres ofSalicorniaplants and bulk soils were collected fromSebkhetandChotthypersaline ecosystems in Tunisia. Depiction of bacterial microbiome composition by Denaturing Gradient Gel Electrophoresis unveiled the occurrence of a high bacterial diversity associated withSalicorniaroot system. A large collection of 475 halophilic and halotolerant bacteria was established fromSalicorniarhizosphere and the surrounding bulk soil, and the bacteria were characterized for the resistance to temperature, osmotic and saline stresses, and plant growth promotion (PGP) features. TwentyHalomonasstrains showed resistance to a wide set of abiotic stresses and were able to perform different PGP activitiesin vitroat 5% NaCl, including ammonia and indole-3-acetic acid production, phosphate solubilisation, and potential nitrogen fixation. By using agfp-labelled strain it was possible to demonstrate thatHalomonasis capable of successfully colonisingSalicorniaroots in the laboratory conditions. Our results indicated that the culturable halophilic/halotolerant bacteria inhabiting salty and arid ecosystems have a potential to contribute to promoting plant growth under the harsh salinity and drought conditions. These halophilic/halotolerant strains could be exploited in biofertilizer formulates to sustain crop production in degraded and arid lands.

Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice

2001 ◽  
Vol 47 (10) ◽  
pp. 916-924 ◽  
Author(s):  
Tika B Adhikari ◽  
C M Joseph ◽  
Guoping Yang ◽  
Donald A Phillips ◽  
Louise M Nelson
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Incidence ◽  
Control Plant ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Bacterial Strains ◽  
Seedling Disease

Of 102 rhizoplane and endophytic bacteria isolated from rice roots and stems in California, 37% significantly (P [Formula: see text] 0.05) inhibited the growth in vitro of two pathogens, Achlya klebsiana and Pythium spinosum, causing seedling disease of rice. Four endophytic strains were highly effective against seedling disease in growth pouch assays, and these were identified as Pseudomonas fluorescens (S3), Pseudomonas tolaasii (S20), Pseudomonas veronii (S21), and Sphingomonas trueperi (S12) by sequencing of amplified 16S rRNA genes. Strains S12, S20, and S21 contained the nitrogen fixation gene, nifD, but only S12 was able to reduce acetylene in pure culture. The four strains significantly enhanced plant growth in the absence of pathogens, as evidenced by increases in plant height and dry weight of inoculated rice seedlings relative to noninoculated rice. Three bacterial strains (S3, S20, and S21) were evaluated in pot bioassays and reduced disease incidence by 50%–73%. Strain S3 was as effective at suppressing disease at the lowest inoculum density (106 CFU/mL) as at higher density (108 CFU/mL or undiluted suspension). This study indicates that selected endophytic bacterial strains have potential for control of seedling disease of rice and for plant growth promotion.Key words: biological control, plant growth promotion, endophytes, rice, seedling disease.

scholarly journals Isolation, Biochemical and Genomic Characterization of Glyphosate Tolerant Bacteria to Perform Microbe-Assisted Phytoremediation

2021 ◽  
Vol 11 ◽  
Author(s):  
Francisco Massot ◽  
Panagiotis Gkorezis ◽  
Jonathan Van Hamme ◽  
Damian Marino ◽  
Bojana Spirovic Trifunovic ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Large Scale ◽  
Agricultural Soils ◽  
Growth Promotion ◽  
Sole Source ◽  
Bacterial Strains ◽  
Positive Effects ◽  
Assisted Phytoremediation

The large-scale use of the herbicide glyphosate leads to growing ecotoxicological and human health concerns. Microbe-assisted phytoremediation arises as a good option to remove, contain, or degrade glyphosate from soils and waterbodies, and thus avoid further spreading to non-target areas. To achieve this, availability of plant-colonizing, glyphosate-tolerant and -degrading strains is required and at the same time, it must be linked to plant-microorganism interaction studies focusing on a substantive ability to colonize the roots and degrade or transform the herbicide. In this work, we isolated bacteria from a chronically glyphosate-exposed site in Argentina, evaluated their glyphosate tolerance using the minimum inhibitory concentration assay, their in vitro degradation potential, their plant growth-promotion traits, and performed whole genome sequencing to gain insight into the application of a phytoremediation strategy to remediate glyphosate contaminated agronomic soils. Twenty-four soil and root-associated bacterial strains were isolated. Sixteen could grow using glyphosate as the sole source of phosphorous. As shown in MIC assay, some strains tolerated up to 10000 mg kg–1 of glyphosate. Most of them also demonstrated a diverse spectrum of in vitro plant growth-promotion traits, confirmed in their genome sequences. Two representative isolates were studied for their root colonization. An isolate of Ochrobactrum haematophilum exhibited different colonization patterns in the rhizoplane compared to an isolate of Rhizobium sp. Both strains were able to metabolize almost 50% of the original glyphosate concentration of 50 mg l–1 in 9 days. In a microcosms experiment with Lotus corniculatus L, O. haematophilum performed better than Rhizobium, with 97% of glyphosate transformed after 20 days. The results suggest that L. corniculatus in combination with to O. haematophilum can be adopted for phytoremediation of glyphosate on agricultural soils. An effective strategy is presented of linking the experimental data from the isolation of tolerant bacteria with performing plant-bacteria interaction tests to demonstrate positive effects on the removal of glyphosate from soils.

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