scholarly journals Genetic characterization of ESBL producing strains of Klebsiella pneumoniae from Tehran hospitals

2010 ◽  
Vol 4 (10) ◽  
pp. 609-615 ◽  
Author(s):  
Mohammad Mehdi Feizabadi ◽  
Samira Mahamadi-Yeganeh ◽  
Akbar Mirsalehian ◽  
Seyed-Mohammad Mirafshar ◽  
Mohaddeseh Mahboobi ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Genetic Characterization ◽  
Dominant Gene ◽  
Confirmatory Test ◽  
Esbl Production ◽  
Disk Diffusion Test ◽  
Extended Spectrum Beta Lactamase

Introduction: This study was conducted to determine the genetic characterization of extended-spectrum beta-lactamase (ESBL) producing strains of Klebsiella  pneumoniae isolated from Iranian patients in hospitals in Tehran. Methodology: Antibiotic susceptibility of 104 isolates was determined using the disk diffusion test. The Minimum Inhibitory Concentrations (MICs) of imipenem and meropenem were determined for isolates showing reduced susceptibility to carbapenems. The phenotypic confirmatory test (PCT) was used to screen the isolates for ESBL production. PCR was used to detect blaSHV, blaTEM and blaCTX-M and the amplicons from selected clones were sequenced. Isolates producing ESBLs were analyzed by pulsed-field gel electrophoresis (PFGE). Results:  One isolate showed resistance to imipenem (MIC = 16 µg/ml). Resistance to amikacin and ciprofloxacin was 44.2% and 25.0%, respectively. ESBL production was detected in 72.1% (n = 75) of isolates. The prevalence of blaSHV, blaTEM and blaCTX-M genes among the isolates was 55.7% (n = 58), 30.7% (n = 32) and 45.2% (n = 47), respectively. The sequencing revealed the amplicons corresponding to bla (TEM-1, TEM-79, SHV-1, SHV-12, SHV-31, CTX-M-15) genes. While the blaCTX-M-15 is the dominant gene among the Iranian isolates, we detected the blaSHV-31 and blaTEM-79 genes for the first time in the country.  PFGE differentiated the 71 ESBL-producing isolates into 62 different genotypes. Clonal dissemination of ESBLs was found in the neonatal intensive care unit and intensive care unit of one hospital. Conclusion: The findings are evidence of the spread of multi-resistant clones of ESBL producers in Tehran hospitals.

Molecular characterization of DHA-1–producing Klebsiella pneumoniae isolates collected during a 4-year period in an intensive care unit

2014 ◽  
Vol 80 (2) ◽  
pp. 159-161 ◽  
Author(s):  
Fabrice Compain ◽  
Dominique Decré ◽  
Jean-Pierre Fulgencio ◽  
Sfia Berraho ◽  
Guillaume Arlet ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Molecular Characterization

scholarly journals Successful Infection Control of an Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae ST307 Outbreak in a Neonatal Intensive Care Unit

2019 ◽  
Author(s):  
Eun-Hwa Baek ◽  
Se-Eun Kim ◽  
Sunjoo Kim ◽  
Seungjun Lee ◽  
Oh-Hyun Cho ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Medical Devices ◽  
Neonatal Intensive Care ◽  
Medical Personnel ◽  
Beta Lactamase ◽  
Clinical Specimens ◽  
Extended Spectrum Beta Lactamase

Abstract Background : We experienced an outbreak of extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae (ESBL-KPN) bacteremia in a neonatal intensive care unit (NICU) starting in August 2017. We implemented an active countermeasure to control the outbreak of ESBL-KPN successfully. Methods: The incidence of ESBL-KPN based on clinical specimens and healthcare-associated infection (HAI) rate were evaluated before and after the initiation of enhanced infection control (IC) practices initiated in January, 2018. Surveillance cultures were carried out for neonates, medical personnel, and NICU environmental samples. Molecular analyses, including pulse-field gel electrophoresis (PFGE), sequence typing, and ESBL genotyping, were performed for the isolated KPN strains. Results: The incidence of ESBL-KPN in clinical specimens decreased from 2.84 to 0.49 per 1,000 patient-days and the HAI rate decreased from 2.43 to 0.0 per 1,000 patient-days after the implementation of enhanced IC procedures. Eleven neonates (11/15, 73.3%), one (1/41, 2.4%) of the medical personnel, and six (6/181, 3.3%) samples from the surroundings and medical devices were positive for ESBL-KPN in the surveillance cultures. All isolates demonstrated the same antibiotic resistance pattern and similar PFGE patterns and were identified as ST307 containing CTX-M-15. Conclusions: Contaminated neonate surroundings and medical devices as well as spreading by medical personnel appeared to be the source of the outbreak of ESBL-KPN. We used an enhanced IC strategy for 3 months and successfully resolved the clonal outbreak of CTX-M-15-producing KPN. ST307 has emerged as an important bacteremia-causing pathogen in the NICU and should be monitored carefully.

scholarly journals Antimicrobial Susceptibility of Metallo-beta-Lactamase Positive and Negative Klebsiella pneumoniae Strains Isolated from Intensive Care Unit Patients

2010 ◽  
Vol 59 (1) ◽  
pp. 67-69 ◽  
Author(s):  
ALICJA SĘKOWSKA ◽  
WALERIA HRYNIEWICZ ◽  
MAREK GNIADKOWSKI ◽  
ALEKSANDER DEPTUŁA ◽  
KRZYSZTOF KUSZA ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
Gram Negative Bacteria ◽  
Beta Lactamase ◽  
Icu Patients ◽  
Extended Spectrum Beta Lactamase ◽  
Genes Encoding ◽  
Commercial Kit

Carbapenem resistance in Gram-negative bacteria is a worldwide increasing and one of the most disturbing problems, given these antibiotics are drugs of choice in the treatment of infections caused by extended-spectrum-beta-lactamase producing strains. In this study the antibiotic susceptibility of metallo-beta-lactamase-positive and negative Klebsiella pneumoniae strains isolated from intensive care unit (ICU) patients was evaluated. The presence of genes encoding MBLs was determined with a commercial kit hyplex MBL ID (BAG HEALTH CARE). The MBL-producing isolates were the first K. pneumoniae isolates of this kind identified in Poland. It seems that methods for detecting MBLs in Enterobacteriaceae should be included in contemporary standards of microbiological diagnostics in the country.

Sign in / Sign up

Export Citation Format

Share Document