scholarly journals Association of Mycobacterium avium subsp. paratuberculosis and SLC11A1 polymorphisms in Sardinian multiple sclerosis patients

2013 ◽  
Vol 7 (03) ◽  
pp. 203-207 ◽  
Author(s):  
Davide Cossu ◽  
Speranza Masala ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Stefania Tranquilli ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Autoimmune Disease ◽  
Mycobacterium Avium ◽  
Humoral Response ◽  
Mycobacterium Avium Subsp ◽  
Nucleotide Polymorphisms ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Multiple Sclerosis Patients

Introduction: Recent findings propose that Mycobacterium avium subsp. paratuberculosis (MAP) infection could act as risk factor in favoring multiple sclerosis (MS) progression. SLC11A1 is a gene associated with mycobacterial survival in the host and it may be involved in the induction and maintenance of autoimmune disease. Methodology: In this preliminary study, 100 MS patients and 100 healthy controls (HCs) from Sardinia were enrolled. Eight single nucleotide polymorphisms (SNPs) in the SLC11A gene were searched by PCR RFLP-genotyping. IS900 specie specific PCR was undertaken to search for MAP presence. Indirect ELISA was performed to asses if MS patients displayed a stronger humoral response against MAP2694 protein compared to the HCs. Results: Only rs2276631 SNP was associated with MS. MAP DNA was detected in 23 out of 100 MS patients (23%) and in 7 out of 100 HCs (7%). A strong humoral response against MAP2694 protein was detected in 36% of MS patients and only in 3% of HCs. A correlation between ELISA sero-positivity and the rs2276631 SNP was also found. Conclusion:  Our preliminary results suggest that the Sardinian population might be prone to develop autoimmune disease due to polymorphisms in immunomodulating the SLC11A1 gene, which is important in the immune response against intracellular bacteria such as MAP.

scholarly journals Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 20
Author(s):  
Luigi De Grossi ◽  
Davide Santori ◽  
Antonino Barone ◽  
Silvia Abbruzzese ◽  
Matteo Ricchi ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Liquid Culture ◽  
Culture Media ◽  
Small Ruminants ◽  
Culture Method ◽  
Mycobacterium Avium Subsp ◽  
Type I ◽  
Culture Methods ◽  
Specific Pcr ◽  
Mycobacterium Avium Subsp Paratuberculosis

Paratuberculosis is a chronic disease of ruminants caused by Mycobacterium avium subsp. Paratuberculosis (MAP). Since isolation of MAP type I (S) is rarely reported in Italy, our research was aimed at isolating, by an inexpensive liquid culture manual method, this type of MAP isolates. At first, we used an ELISA to point out to serologically positive samples from five flocks. Secondly, we used a fecal direct IS900-qPCR on the ELISA positive samples, in order to detect shedder animals. Feces from IS900-qPCR positive samples were inoculated in solid and liquid culture media. IS900-qPCR was further used to test the growth of MAP isolates in liquid medium, which were further confirmed by f57-qPCR and submitted to typing by specific PCR in order to identify the MAP type. Twenty-eight samples (24 fecal and four tissutal samples) were processed by culture methods, resulting in the isolation of six type I MAP field isolates. Notably, no isolates were recovered by solid media, underlining the utility of this liquid method. Few data about this type of MAP are currently available in Italy, and further analyses should be carried out in order to study the origin and epidemiology of type I strains circulating in Italy.

scholarly journals Association of Mycobacterium avium subsp. paratuberculosis with Multiple Sclerosis in Sardinian Patients

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18482 ◽  
Author(s):  
Davide Cossu ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Speranza Masala ◽  
Niyaz Ahmed ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

Are Mycobacterium avium subsp. paratuberculosis and Epstein–Barr virus triggers of multiple sclerosis in Sardinia?

2012 ◽  
Vol 18 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Davide Cossu ◽  
Speranza Masala ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Jessica Frau ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Avium ◽  
Epstein Barr Virus ◽  
Mycobacterium Avium Subsp ◽  
Healthy Controls ◽  
Humoral Immune ◽  
Barr Virus ◽  
Protein Map ◽  
Epstein Barr ◽  
Mycobacterium Avium Subsp Paratuberculosis

Sardinia acts as an ideal setting for multiple sclerosis (MS) studies because its prevalence of MS is one of the highest worldwide. Several pathogens have been investigated amongst 119 Sardinian MS patients and 117 healthy controls to determine whether they might have a role in triggering MS in genetically predisposed individuals. Mycobacterium avium subsp. paratuberculosis (MAP) and Epstein Barr virus DNA were detected in 27.5% and 17.3%, respectively, of the MS patients. Moreover an extremely high humoral immune response against MAP recombinant protein MAP FprB (homologous to human myelin P0) was observed, whereas no significant results were found against Mycobacterium tuberculosis FprA and Helicobacter pylori HP986 protein.

Lipopentapeptide induces a strong host humoral response and distinguishes Mycobacterium avium subsp. paratuberculosis from M. avium subsp. avium

Vaccine ◽  
2008 ◽  
Vol 26 (2) ◽  
pp. 257-268 ◽  
Author(s):  
Franck Biet ◽  
Sylvie Bay ◽  
Virginie C. Thibault ◽  
Daniel Euphrasie ◽  
Maggy Grayon ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Humoral Response ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis
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