scholarly journals Osteoarticular tuberculosis – diagnostic solutions in a disease endemic region

2009 ◽  
Vol 3 (07) ◽  
pp. 511-516 ◽  
Author(s):  
Vikas Agashe ◽  
Shubhada Shenai ◽  
Ganesh Mohrir ◽  
Minal Deshmukh ◽  
Anita Bhaduri ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Bone Diseases ◽  
Drug Resistant ◽  
Endemic Region ◽  
Mycobacterial Culture ◽  
Osteoarticular Tuberculosis ◽  
Private And Public ◽  
Polymerase Chain ◽  
Negative Controls

Background: We conducted a study of osteoarticular tuberculosis in patients from private and public settings in a disease endemic area. Our objective was to assess the role of mycobacterial culture and polymerase chain reaction (PCR) in the diagnosis of osteoarticular tuberculosis (TB) in settings where only clinical and imaging diagnosis form the basis for treatment. Methodology: Ninety-three consecutive specimens collected from clinically suspected patients of osteoarticular TB were screened for bacterial culture, mycobacterial culture and in-house nested PCR. In addition, specimens were examined by imaging and histopathology. Ten specimens collected from patients suffering from other bone diseases were included as negative controls. Results: Of the 93 clinically suspected TB patients, mycobacterial culture was positive for Mycobacterium tuberculosis (MTB) in 47 (51%) patients who were confirmed as definite TB cases. Of the remaining patients, 16 (17%) were diagnosed as probable, 19 (20%) as possible, and 11 (12%) as only clinically suspected TB cases. In-house nested PCR was positive in 65 (70%) cases. Fifteen patients were resistant to one or more anti-tuberculous drugs; twelve patients were multi-drug resistant, two of whom were extensively drug resistant. Conclusion: Mycobacterial cultures using liquid media with susceptibility should form the backbone of management of osteoarticular TB. Nested PCR enhances the sensitivity if performed in addition to culture.

scholarly journals Molecular investigation and geographical distribution of Leishmania spp infection in stray and owned cats (Felis catus) in Thessaly, central Greece

2018 ◽  
Vol 68 (1) ◽  
pp. 27 ◽  
Author(s):  
A. GIANNAKOPOULOS ◽  
C. N. TSOKANA ◽  
E. PAPADOPOULOS ◽  
V. SPYROU ◽  
D. C. CHATZOPOULOS ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Nested Pcr ◽  
Living Conditions ◽  
Control Measures ◽  
Geographical Information ◽  
Endemic Region ◽  
Central Greece ◽  
Molecular Investigation ◽  
Leishmania Spp

In this study we aimed to determine the prevalence of Leishmania spp infection and to report the geographical distribution of Leishmania spp infected cats from Thessaly, central Greece, an endemic region for canine and human leishmaniosis. Blood samples were collected from a total of 150 cats (34 stray and 116 owned) from the area of Thessaly, i.e. Larissa (n=6), Volos (n=94), Trikala (n=30) and Karditsa (n=20). Data on signalment and living conditions were registered for each cat. The samples were tested by Internal Transcribed Spacer 1 nested PCR (ITS1 nested PCR). Geographical Information System (GIS) was used to define the geographical distribution of the Leishmania spp infected cats in relation to the land uses and the altitude. In total, 13.3% (95% CI: 8.3-19.8) of the sampled cats were found positive for Leishmania spp DNA. More precisely, the results are 12.8% (95% CI 6.8-21.2) in Volos, 20% (95% CI 6.8-21.2) in Trikala and 10% (95% CI 12-31.7) in Karditsa while none of the six cats examined from the region of Larissa was found PCR positive. The Leishmania spp infected cats were found in artificial surfaces and associated areas and in cultivated and managed areas with a mean altitude of 81,7 m above sea level (range 14 - 225 ± 51.57 SD). No significant correlation was found between Leishmania spp infection and signalment or living conditions, thus suggest ing that the infection is uniformly distributed among the screened feline population. The findings of this study along with the occurrence of competent vectors of Leishmania infantum in Thessaly, support the hypothesis that cats may serve as a reservoir for Leishmania spp in this region and highlight the need for surveillance in feline population and implementation of control measures. However, the epidemiological role of cats in the maintenance and transmission of Leishmania spp has yet to be defined and deserves further investigation.

scholarly journals High number of asymptomatic dogs as leptospiral carriers in an endemic area indicates a serious public health concern

2017 ◽  
Vol 145 (9) ◽  
pp. 1852-1854 ◽  
Author(s):  
R. SANT'ANNA ◽  
A. S. VIEIRA ◽  
J. GRAPIGLIA ◽  
W. LILENBAUM
Keyword(s):  
Public Health ◽  
Endemic Area ◽  
Urine Samples ◽  
Health Concern ◽  
Endemic Region ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Knowing That ◽  
Polymerase Chain

SUMMARYAsymptomatic dogs can be potential hosts of leptospirosis. However, the extension of this phenomenon in endemic areas has not yet been clearly defined. This study is aimed at evaluating the role of asymptomatic dogs as carriers of Leptospira in an endemic area of Brazil. A total of 131 male dogs without apparent leptospirosis symptoms were included in the study based on clinical and hematologic exams. Serum and urine samples were collected for microscopic agglutination tests (MAT) and polymerase chain reactions (PCR) targeted the LipL32 gene, respectively. Forty-two dogs (32·1%) presented seroreactivity (titres ⩾100). The serogroup Icterohaemorrhagiae was predominant, representing 92·7% of the seropositive samples. Overall, leptospiral DNA was detected on 26 urine samples (19·8%). PCR positivity was more common (28·6%) on seropositive dogs than on seronegative (15·7%) ones. Nevertheless, MAT was not correlated to PCR (P > 0·05). Age was not associated with seroreactivity, but dogs older than 5 years of age had 4·07 more chances (odds ratio) of being carriers (PCR positive) than younger ones. Although the fact of knowing that asymptomatic dogs can act as leptospiral carriers is not new, the extension of this fact is impressive in an endemic region, and its role and impact on public health cannot be neglected.

scholarly journals The role of polymerase chain reaction in the management of osteoarticular tuberculosis

2007 ◽  
Vol 33 (3) ◽  
pp. 801-805 ◽  
Author(s):  
Vivek Pandey ◽  
Kiran Chawla ◽  
Kiran Acharya ◽  
Sripathi Rao ◽  
Sugandhi Rao
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Osteoarticular Tuberculosis ◽  
Polymerase Chain

scholarly journals Lack of Association between Chlamydophila psittaci and Ocular Adnexal MALT Lymphoma in Korean Patients—Is the Geographic or Genetic Difference Significant?

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2069
Author(s):  
Uiju Cho ◽  
Inju Cho ◽  
Sung Hak Lee ◽  
Suk Woo Yang ◽  
Seok Goo Cho ◽  
...  
Keyword(s):  
Malt Lymphoma ◽  
Marginal Zone ◽  
Genetic Difference ◽  
Etiologic Factor ◽  
Detection Rates ◽  
Negative Results ◽  
Polymerase Chain ◽  
Primer Sets ◽  
Negative Controls

Clamydophila psittaci (C. psittaci) has been proposed to be an etiologic factor in extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) in the ocular adnexa. However, the pathogenetical significance of the infection has not been fully elucidated. Many previous studies have shown controversial results regarding C. psittaci detection rates in said patients, ranging from 0 to 87%. We investigated the presence of C. psittaci in a single institutional cohort (n = 150) of ocular adnexal MALT lymphoma (OAML) patients in Korea. We tried to exclude the methodological biases derived from the different primer sets in polymerase chain reaction-based studies. For that reason, we applied five sets of primers, including four previously reported and one newly designed primer set. There was no case of C. psittaci-positive OAML in repeated trials validated with appropriate positive and negative controls. All 150 cases showed negative results with five primer sets. These results suggest that the pathogenetic role of C. psittaci in ocular adnexal MALT lymphoma might have been overestimated to date, at least in the Korean population. Therefore, the molecular diagnosis of C. psittaci is considered a very low priority.

Molecular Detection of Adenoviruses in the Sinus Tissues of Patient by Nested-PCR in Shiraz, Southwest Iran

2019 ◽  
Author(s):  
Shahab Mahmoudvand ◽  
Mohammad Kord ◽  
Neda Pirbonyeh ◽  
Afagh Moattari ◽  
Somayeh Shokri ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Molecular Detection ◽  
Viral Infections ◽  
Globin Gene ◽  
Nested Polymerase Chain Reaction ◽  
High Prevalence ◽  
Polymerase Chain ◽  
Southwest Iran

Background and Aims: Rhinosinusitis is an inflammation of the mucous membrane that may be caused by infectious agents such as bacteria, fungi and viruses. Few studies have been carried out on the role of viruses in Rhinosinusitis patients .The aim of this study was the molecular detection of Adenoviruses in sinus tissues by nested polymerase chain reaction (PCR) in Shiraz.Materials and Methods: In the present study, 103 paraffin-embedded biopsy specimens of sinus tissues were subjected to DNA extraction and tested for adenovirus DNA using Nested PCR. The amplification of a β-globin gene by PCR-based method was used to confirm the quality of extracted DNA.Results: A total of 103 samples of sinus tissues were examined. Of these patients, 50 (48.54%) were male and the rest were female (51.46%). The patients’ age ranged between 2 and 82 years and the mean age was 42.15±1.56 years. The adenovirus DNA was detected in 13 of 103 (12.6%) samples.Conclusions: The results of this study showed that Adenoviruses have high prevalence in rhinosinusitis patients. As a results, it is an important to investigate clinical significance of viral infections especially Adeno viruses in these patients.

دور علماء الإسلام في مواجهة التحديات بين المسلمين في الصين (The Role of The Islamic Scholars In Encountering The Challenges Among The Muslims In China)

1970 ◽  
Vol 13 (2) ◽  
pp. 83-116
Author(s):  
يونس عبد الله ما تشنغ بين الصيني
Keyword(s):  
Legal System ◽  
Chinese Language ◽  
Public Life ◽  
Islamic Culture ◽  
Private And Public ◽  
Islamic Faith

الملخّص إن بقاء الإسلام، ورغبة المسلمين في الحفاظ على عقيدة الإسلام، وشريعته السمحاء في الصين راجعة إلى جهود علمائنا الأجلاء الذين نَهَلُوا العلم الصافي من مَعِينِ القرآن والسنة. وخدمتهم من خلال ترجمة معاني القرآن الكريم، وتبسيط العقيدة والشريعة باللغة الصينية خير دليل على ذلك. ويحاول الباحث تسليط الضوء من خلال هذا البحث على طبيعة الإسلام في أرض الصين، كاشفا أمر وضع الإسلام وطبيعة حال المسلمين، وتحدياتهم قديما وحديثا، مبينا محاولتهم على حفاظ دين الإسلام، وأداء شعائره. ويؤمن الباحث من خلال توصيف حالة الإسلام والمسلمين، أن صلاح المسلمين، وبقاءهم كأمة مسالمة لا رغبة لها؛ إلا في الإصلاح، والتعمير في الأرض، فهو لا يتحقق إلا بإصلاح النفس، وعودتها إلى طاعة الله سرا وعلانية دون الإنغماس في تحقيق الرغبات المادية، وإشباع المطامع الشهوانية من خلال جمع حطام الدنيا دون الالتفات إلى حلال وحرام، وطاعة ومعصية.    الكلمات المفتاحيّة: جهود العلماء، ثقافة الإسلام، مصادر الإسلام الأصلية، التحديات، الصين، الدعوة.                                                                                             Abstract The continuation of Islam in China and the aspiration of the Muslims to maintain Islamic faith and its true tolerant legal system retract to the struggles of our respected scholars who learnt the knowledge of the Qurʼan and the Prophetic traditions (al-Sunnah). The services they rendered in translating the meaning of the Qurʼan, simplifying the creed and the legal system of Islam into Chinese language are good indications in that context. In this paper, the researcher is trying to highlight the normal nature of Islam in China by exploring the position and nature of the Muslims, their contemporary and past challenges, and revealing their attempts to preserve the religion of Islam in discharging the religious rites. Through the depiction of Islam and the Muslims, the researcher believes that the wellbeing of Muslims and their continuous survival to be a peace-loving nation could not be achieved without the reform and proper development through self-reformation and its return to full submission to Allah both in private and public life, and without indulging in attainment of material desires and satiating the lust of accumulating ephemeral materials of this world without paying any heed to lawfulness or unlawfulness, or to being obedient or disobedient. Keywords: Effort of the Scholars, Islamic Culture, Noble Origin of Islam, Challenges, Propagation of the Religion.

دور علماء الإسلام في مواجهة التحديات بين المسلمين في الصين The Role of The Islamic Scholars In Encountering The Challenges Among The Muslims In China

1970 ◽  
Vol 13 (1) ◽  
pp. 83-116
Author(s):  
يونس عبد الله ما تشنغ بين الصيني
Keyword(s):  
Legal System ◽  
Chinese Language ◽  
Public Life ◽  
Islamic Culture ◽  
Private And Public ◽  
Islamic Faith

الملخّصإن بقاء الإسلام، ورغبة المسلمين في الحفاظ على عقيدة الإسلام، وشريعته السمحاء في الصين راجعة إلى جهود علمائنا الأجلاء الذين نَهَلُوا العلم الصافي من مَعِينِ القرآن والسنة. وخدمتهم من خلال ترجمة معاني القرآن الكريم، وتبسيط العقيدة والشريعة باللغة الصينية خير دليل على ذلك. ويحاول الباحث تسليط الضوء من خلال هذا البحث على طبيعة الإسلام في أرض الصين، كاشفا أمر وضع الإسلام وطبيعة حال المسلمين، وتحدياتهم قديما وحديثا، مبينا محاولتهم على حفاظ دين الإسلام، وأداء شعائره. ويؤمن الباحث من خلال توصيف حالة الإسلام والمسلمين، أن صلاح المسلمين، وبقاءهم كأمة مسالمة لا رغبة لها؛ إلا في الإصلاح، والتعمير في الأرض، فهو لا يتحقق إلا بإصلاح النفس، وعودتها إلى طاعة الله سرا وعلانية دون الإنغماس في تحقيق الرغبات المادية، وإشباع المطامع الشهوانية من خلال جمع حطام الدنيا دون الالتفات إلى حلال وحرام، وطاعة ومعصية.   الكلمات المفتاحيّة: جهود العلماء، ثقافة الإسلام، مصادر الإسلام الأصلية، التحديات، الصين، الدعوة.                                                                                   AbstractThe continuation of Islam in China and the aspiration of the Muslims to maintain Islamic faith and its true tolerant legal system retract to the struggles of our respected scholars who learnt the knowledge of the Qurʼan and the Prophetic traditions (al-Sunnah). The services they rendered in translating the meaning of the Qurʼan, simplifying the creed and the legal system of Islam into Chinese language are good indications in that context. In this paper, the researcher is trying to highlight the normal nature of Islam in China by exploring the position and nature of the Muslims, their contemporary and past challenges, and revealing their attempts to preserve the religion of Islam in discharging the religious rites. Through the depiction of Islam and the Muslims, the researcher believes that the wellbeing of Muslims and their continuous survival to be a peace-loving nation could not be achieved without the reform and proper development through self-reformation and its return to full submission to Allah both in private and public life, and without indulging in attainment of material desires and satiating the lust of accumulating ephemeral materials of this world without paying any heed to lawfulness or unlawfulness, or to being obedient or disobedient.Keywords: Effort of the Scholars, Islamic Culture, Noble Origin of Islam, Challenges, Propagation of the Religion.

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