scholarly journals Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton’s Jelly-Derived Stem Cells

2019 ◽  
Vol 7 (10) ◽  
pp. 1572-1575 ◽  
Author(s):  
Nora Ariyati ◽  
Kusworini Kusworini ◽  
Nurdiana Nurdiana ◽  
Yohanes Widodo Wirohadidjojo
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Growth Factor ◽  
Conditioned Medium ◽  
Sandwich Elisa ◽  
Wharton’S Jelly ◽  
Crosslinking Degree ◽  
Wharton's Jelly ◽  
Low Degree ◽  
Tgf Β1

BACKGROUND: Presently, the application of stem cells and their paracrine effect for anti-ageing therapy has commenced. Wharton's jelly-derived stem cell conditioned medium (WJSCs-CM) is renowned for increasing proliferation, migrate ageing skin fibroblasts and increase consumption of extracellular transforming growth factor-β (TGF-β). With more than 85% of frequently used dermal filler procedures are hyaluronic acid fillers (HA), a mixture of both with optimal HA crosslinking degree has not yet been identified. AIM: This study aimed to determine the discrepancies in the results of various HA crosslinking degree in WJSCs-CM concerning various levels of growth factors (GF). METHODS: Conditioned medium was obtained from mesenchymal stem cells Wharton’s jelly of the newborn umbilical cord with caesarean section procedure, fabricated with hypoxia method (HCM). HA was obtained from preparations on the market with crosslinking degrees of 3%, 4%, and 10%. GF levels were measured using sandwich ELISA method based on the protocol provided by anti-TGF-β1, platelet-derived growth factor (PDGF), and basic fibroblast growth factor (bFGF) antibody producers (Cloud-Clone Corp®, Texas, USA). RESULTS: Low degree HA crosslinking (3% and 4%) elevated TGF-β1 release in WJSCs-CM. HA crosslinking did not provoke increased levels of PDGF and bFGF in WJSCs-CM, both at low and higher degrees. CONCLUSION: Low degree HA crosslinking induced the increase of TGF-β1 release in WJSCs-CM.

scholarly journals Embryonic stem cells conditioned medium enhances Wharton’s jelly-derived mesenchymal stem cells expansion under hypoxic condition

2014 ◽  
Vol 67 (3) ◽  
pp. 493-505 ◽  
Author(s):  
Patcharee Prasajak ◽  
Piyaporn Rattananinsruang ◽  
Kamonnaree Chotinantakul ◽  
Chavaboon Dechsukhum ◽  
Wilairat Leeanansaksiri
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Embryonic Stem Cells ◽  
Conditioned Medium ◽  
Embryonic Stem ◽  
Hypoxic Condition ◽  
Wharton’S Jelly ◽  
Wharton's Jelly

scholarly journals Differentiation of Human Mesenchymal Stem Cells from Wharton’s Jelly Towards Neural Stem Cells Using a Feasible and Repeatable Protocol

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 739
Author(s):  
Ewa Kruminis-Kaszkiel ◽  
Adam Osowski ◽  
Ewa Bejer-Oleńska ◽  
Mariusz Dziekoński ◽  
Joanna Wojtkiewicz
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Neural Stem Cells ◽  
Wharton’S Jelly ◽  
Pcr Analysis ◽  
Mrna Levels ◽  
Wharton's Jelly ◽  
Differentiated Cells ◽  
Neural Markers

The transplantation of neural stem cells (NSCs) capable of regenerating to the cells of the central nervous system (CNS) is a promising strategy in the treatment of CNS diseases and injury. As previous studies have highlighted mesenchymal stem cells (MSCs) as a source of NSCs, this study aimed to develop a feasible, efficient, and reproducible method for the neural induction of MSCs isolated from Wharton’s jelly (hWJ-MSCs). We induced neural differentiation in a monolayer culture using epidermal growth factor, basic fibroblast growth factor, N2, and B27 supplements. This resulted in a homogenous population of proliferating cells that expressed certain neural markers at both the protein and mRNA levels. Flow cytometry and immunocytochemistry confirmed the expression of neural markers: nestin, sex-determining region Y (SRY) box 1 and 2 (SOX1 and SOX2), microtubule-associated protein 2 (MAP2), and glial fibrillary acidic protein (GFAP). The qRT-PCR analysis revealed significantly enhanced expression of nestin and MAP2 in differentiated cells. This study confirms that it is possible to generate NSCs-like cells from hWJ-MSCs in a 2D culture using a practical method. However, the therapeutic effectiveness of such differentiated cells should be extended to confirm the terminal differentiation ability and electrophysiological properties of neurons derived from them.

scholarly journals Erratum: Human Wharton's Jelly Stem Cells and Its Conditioned Medium Enhance Healing of Excisional and Diabetic Wounds

2017 ◽  
Vol 118 (9) ◽  
pp. 3016-3016
Author(s):  
Chui-Yee Fong ◽  
Kimberley Tam ◽  
Suganya Cheyyatraivendran ◽  
Shu-Uin Gan ◽  
Kalamegam Gauthaman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Diabetic Wounds

scholarly journals Enhanced Hepatogenic Differentiation of Human Wharton’s Jelly–Derived Mesenchymal Stem Cells by Using Three-Step Protocol

2019 ◽  
Vol 20 (12) ◽  
pp. 3016 ◽  
Author(s):  
Wachira Panta ◽  
Sumeth Imsoonthornruksa ◽  
Ton Yoisungnern ◽  
Sanong Suksaweang ◽  
Mariena Ketudat-Cairns ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Glycogen Storage ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Lineage Differentiation ◽  
Cell Based Therapy ◽  
Hepatogenic Differentiation ◽  
Pre Treatment

Currently, human Wharton’s jelly-derived mesenchymal stem cells (hWJ-MSCs) are an attractive source of stem cells for cell-based therapy, owing to their ability to undergo self-renewal and differentiate into all mesodermal, some neuroectodermal, and endodermal progenies, including hepatocytes. Herein, this study aimed to investigate the effects of sodium butyrate (NaBu), an epigenetic regulator that directly inhibits histone deacetylase, on hepatic endodermal lineage differentiation of hWJ-MSCs. NaBu, at 1 mM, optimally promoted endodermal differentiation of hWJ-MSCs, along with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) supplementation (EGF + bFGF + 1 mM NaBu). CXCR4, HNF3β, SOX17 (endodermal), and GATA6 (mesendodermal) mRNAs were also up-regulated (p < 0.001). Immunocytochemistry and a Western blot analysis of SOX17 and HNF3β confirmed that the EGF + bFGF + 1 mM NaBu condition was appropriately pre-treated with hWJ-MSCs before hepatogenic differentiation. Furthermore, the hepatogenic medium + NaBu pre-treatment up-regulated hepatoblast (AFP and HNF3β) and hepatic (CK18 and ALB) markers, and increased the proportion of mature hepatocyte functions, including G6P, C/EBPα, and CYP2B6 mRNAs, glycogen storage and urea secretion. The hepatogenic medium + NaBu in the pre-treatment step can induce hWJ-MSC differentiation toward endodermal, hepatoblastic, and hepatic lineages. Therefore, the hepatogenic medium + NaBu pre-treatment for differentiating hWJ-MSCs could represent an alternative protocol for cell-based therapy and drug screening in clinical applications.

Human Wharton's Jelly Stem Cells, its Conditioned Medium and Cell-Free Lysate Inhibit the Growth of Human Lymphoma Cells

2014 ◽  
Vol 10 (4) ◽  
pp. 573-586 ◽  
Author(s):  
Hao Daniel Lin ◽  
Chui Yee Fong ◽  
Arijit Biswas ◽  
Mahesh Choolani ◽  
Ariff Bongso
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Wharton’S Jelly ◽  
Lymphoma Cells ◽  
Wharton's Jelly ◽  
Human Lymphoma
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