Caspase-3 inhibitor prevents the apoptosis of brain tissue in rats with acute cerebral infarction

Objective: The purpose of this paper was to study the protective effect of paeoniflorin on acute cerebral ischemia. The animal model of cerebral infarction induced by Middle Cerebral Artery Occlusion (MCAO) was blocked by the suture method. Sixty SD rats were randomly divided into the shame group, MCAO group, paeoniflorin (60, 120, 240 mg/kg, respectively) and Nimodipine (NMDP) group (n = 10 per group). Methods: The rats were intragastrically administered immediately after the operation. After 7 days of gavage, the brains were decapitated at 24 h. Hematoxylin and Eosin (HE) staining was used to observe the degree of cell damage in the cerebral cortex of rats. Immunohistochemistry was used to detect silver plating and to observe changes in nerve cells. Rats in the model group showed obvious symptoms of neurological deficits, such as the ischemic morphological changed, the Malondialdehyde (MDA), Lactate Dehydrogenase (LD) content and lactate dehydrogenase (LDH) activity were significantly increased in the ischemic brain tissue, while the Superoxide Dismutase (SOD) activity was decreased. Results: The decrease in Na+-K+-ATPase activity was significantly lower than that in the sham group. The neurological symptoms and signs of MCAO in the different doses of paeoniflorin group were improved, and the neuronal edema in the cortical area was alleviated. The activities of SOD, LDH and Na+-K+-ATPase were significantly increased, and the contents of MDA and LD were decreased. Conclusion: Therefore, paeoniflorin could alleviate the degree of tissue damage in rats with acute cerebral infarction, inhabit the formation of free radicals in the brain tissue after ischemia, and reduce the degree of lipid peroxidation. Thus, the degree of cell damage was reduced greatly and a protective effect was showed on cerebral ischemia.

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Dynamics of Polymorphonuclear Leukocyte Accumulation in Acute Cerebral Infarction and Their Correlation With Brain Tissue Damage

Stroke ◽

10.1161/01.str.27.10.1739 ◽

1996 ◽

Vol 27 (10) ◽

pp. 1739-1743 ◽

Cited By ~ 134

Author(s):

S.E. Akopov ◽

N.A. Simonian ◽

G.S. Grigorian

Keyword(s):

Cerebral Infarction ◽

Brain Tissue ◽

Tissue Damage ◽

Polymorphonuclear Leukocyte ◽

Acute Cerebral Infarction ◽

Leukocyte Accumulation ◽

Brain Tissue Damage

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Ependymal Response in Acute Cerebral Infarction

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050263 ◽

1974 ◽

Vol 32 ◽

pp. 50-51

Author(s):

Ronald F. Dodson ◽

Yukio Tagashira

Keyword(s):

Cerebral Infarction ◽

Caudate Nucleus ◽

Brain Tissue ◽

Squirrel Monkey ◽

Blood Brain Barrier ◽

Lateral Ventricle ◽

Brain Parenchyma ◽

Acute Cerebral Infarction ◽

Brain Barrier ◽

Ependymal Lining

The ependymal complex (subependymal, ependymal, and supraependymal) forms the complex sheath which separates brain parenchyma from ventricular contents. This structure forms a functionally less efficient counterpart to the blood-brain-barrier. That area of the ependymal lining which separates the caudate nucleus from the cavity of the lateral ventricle has recently been described in the adult squirrel monkey. This area was of particular interest since the underlying brain tissue was shown to be of great susceptibility to periods of ischemic as well as hypoxic/ischemic insults.

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Effects of near-far acupuncture on neuronal function and expression of apoptosis-related protein Bax/Bcl-2/Cleaved caspase-3 in rats with ischemic stroke

Acupuncture & Electro-Therapeutics Research ◽

10.3727/036012921x16112663844879 ◽

2021 ◽

Vol 45 (2) ◽

pp. 73-86

Author(s):

Wang Jian ◽

Zhang Can ◽

Yang Jun ◽

Xing Liwei ◽

Zhang Kun ◽

...

Keyword(s):

Ischemic Stroke ◽

Cerebral Infarction ◽

Brain Tissue ◽

Caspase 3 ◽

Neurological Function ◽

Acupuncture Group ◽

Sham Operation ◽

Model Group ◽

Ischemic Brain ◽

Ischemic Brain Tissue

Objectives To explore the effects of electroacupuncture on nerve function in rats with ischemic stroke and its mechanism of anti-apoptosis. Methods A total of 80 SPF male SD rats were randomly divided into sham operation group, model group, sham electroacupuncture group, acupuncture group, electroacupuncture group and 16 rats in each group. The rat model of left ischemic stroke was prepared by suture embolization. In the sham group, the left common carotid artery was isolated only and no other treatment was given. In the electroacupuncture group, "Baihui" and "Mingmen" were selected for acupuncture, followed by dilatation wave, frequency 2Hz/100Hz, intensity 1mA, and electroacupuncture for 30min. The sham electroacupuncture group was the same as the electroacupuncture group in acupoint electroacupuncture group was the same as the electroacupuncture group in acupoint selection. The electroacupuncture group was only inserted subcutaneously and then connected with the electroacupuncture group without power supply, and fixed for 30 minutes. The electroacupuncture group and the acupuncture group received electroacupuncture treatment once, for a total of 14 days, 1d after modeling. The Improved Neurological Impairment scale (mNSS) was used to evaluate the degree of neurological impairment in each group after anesthesia and wakefulness. The percentage of cerebral infarction area was determined by TTC staining. HE staining and Nissl staining were used to observe the pathological changes of ischemic brain tissue. The level of apoptosis in ischemic brain tissue was detected by TUNEL assay. Western blot was used to detect protein expression of Bax, Bcl-2 and Cleaved caspase-3 in ischemic brain tissue. Results Compared with the sham operation group, neurological function score, percentage of cerebral infarction area and apoptosis level in the model group were significantly increased (all P < 0.01). Compared with the model group, neurological function score, percentage of cerebral infarction area and apoptosis index of acupuncture group and electroacupuncture group were decreased (all P <0.05). Compared with the model group, the expression levels of Bcl-2 protein in ischemic brain tissue of rats with ischemic stroke were up-regulated in the acupuncture group and electroacupuncture group to different degrees, while the expression levels of Bax and Cleaved caspase-3 protein were down-regulated in the electroacupuncture group. Conclusion Electroacupuncture may inhibit Bax, Cleaved caspase-3 and up-regulate the expression of Bcl-2 against neuronal apoptosis, thereby improving the neurological function injury of ischemic stroke rats.

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Effect of baculovirus P35 protein on apoptosis in brain tissue of rats with acute cerebral infarction

Genetics and Molecular Research ◽

10.4238/2015.august.10.16 ◽

2015 ◽

Vol 14 (3) ◽

pp. 9353-9360 ◽

Cited By ~ 5

Author(s):

J.F. Ji ◽

X.H. Ma

Keyword(s):

Cerebral Infarction ◽

Brain Tissue ◽

Acute Cerebral Infarction ◽

Baculovirus P35

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Treatment of experimental cerebral infarction in rats with levodopa or with glycerol

Journal of Neurosurgery ◽

10.3171/jns.1978.48.6.0962 ◽

1978 ◽

Vol 48 (6) ◽

pp. 962-969 ◽

Cited By ~ 4

Author(s):

Pava Popovic ◽

Vojin Popovic ◽

Richard Schaffer ◽

Carl H. Sutton

Keyword(s):

Cerebral Infarction ◽

Brain Tissue ◽

Survival Rates ◽

Acute Cerebral Infarction ◽

Pathological Findings ◽

Carbon Microspheres ◽

Content Type ◽

Animal Survival ◽

Experimental Cerebral Infarction ◽

Main Factor

✓ Administration of large amounts of levodopa did not improve survival rates of rats after acute cerebral infarction induced by injection of carbon microspheres. However, when 10% glycerol was used, the number of rats that survived after cerebral infarction was significantly greater than in the control or in the levodopa-treated rats. Combination of levodopa and glycerol therapy also significantly improved the survival rate of infarcted animals. It appears that glycerol alone is the main factor in eliciting this beneficial effect. Pathological findings (gross or microscopic) indicate striking changes in brain tissue after embolization. Development of brain edema of the infarcted left hemisphere corresponded to the type of treatment and to the length of animal survival. Brain-tissue histology indicates that glycerol-treated animals developed less severe edema and had less tissue disruption than control animals. The results suggest that treatment of edema should be one of the primary steps in therapy after acute cerebral infarction.

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