Effect of Hath1 on the proliferation and apoptosis of cutaneous squamous cell carcinoma in vitro

Background: Cutaneous squamous cell carcinoma (cSCC) incidence continues to rise with increasing morbidity and mortality, with limited treatment options for advanced disease. Future improvements in targeted therapy will rely on advances in genomic/transcriptomic understanding and the use of model systems for basic research. We describe here the panel of 16 primary and metastatic cSCC cell lines developed and characterised over the past three decades in our laboratory in order to provide such a resource for future preclinical research and drug screening. Methods: Primary keratinocytes were isolated from cSCC tumours and metastases, and cell lines were established. These were characterised using short tandem repeat (STR) profiling and genotyped by whole exome sequencing. Multiple in vitro assays were performed to document their morphology, growth characteristics, migration and invasion characteristics, and in vivo xenograft growth. Results: STR profiles of the cSCC lines allow the confirmation of their unique identity. Phylogenetic trees derived from exome sequence analysis of the matched primary and metastatic lines provide insight into the genetic basis of disease progression. The results of in vivo and in vitro analyses allow researchers to select suitable cell lines for specific experimentation. Conclusions: There are few well-characterised cSCC lines available for widespread preclinical experimentation and drug screening. The described cSCC cell line panel provides a critical tool for in vitro and in vivo experimentation.

Download Full-text

Effects of Vitamin K3 Combined with UVB on the Proliferation and Apoptosis of Cutaneous Squamous Cell Carcinoma A431 Cells

OncoTargets and Therapy ◽

10.2147/ott.s228792 ◽

2020 ◽

Vol Volume 12 ◽

pp. 11715-11727

Author(s):

Shangyuchen Shi ◽

Gang Zheng ◽

Chunsheng Yang ◽

Xi Chen ◽

Qiuyue Yan ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma ◽

Vitamin K3 ◽

A431 Cells ◽

Proliferation And Apoptosis

Download Full-text

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinoma

Acta Pharmaceutica ◽

10.2478/v10007-011-0004-8 ◽

2011 ◽

Vol 61 (1) ◽

pp. 63-71 ◽

Cited By ~ 12

Author(s):

Vandana Gupta ◽

Ramesh Agrawal ◽

Piyush Trivedi

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Skin Permeation ◽

Research Work ◽

Cutaneous Squamous Cell Carcinoma ◽

Histopathological Study ◽

Target Cells ◽

Micronucleus Formation

Reduction in cisplatin genotoxicity (micronucleus formation) in non target cells of mice by protransfersome gel formulation used for management of cutaneous squamous cell carcinomaCisplatin-loaded protransfersome system was prepared and characterized forin vitrodrug permeation, drug deposition and antitumor effect. A histopathological study and a genotoxicity study were also done. The skin permeation data of cisplatin from protransfersome gel formulation revealed 494.33 ± 11.87 μg cm-2, which was significantly higher than that from the control plain drug solution in 0.9 % NaCl (p< 0.001). Untreated group of animals showed invasive moderately differentiated keratinizing squamous cell carcinoma (malignant stage). However, with cisplatin loaded protransfersome gel system simple epithelial hyperplasia (pre-cancerous stage) with no cancerous growth was observed. Also, a significant induction in micronucleus formation was found in the group that was treated with injectable intraperitoneal cisplatin preparation in 0.9 % saline as compared to the group treated with topical protransfersome gel formulation. The findings of this research work appear to support improved, site-specific and localized drug action in the skin, thus providing a better option for dealing with skin related problems like squamous cell carcinoma.

Download Full-text

Topical Application of Houttuynia Cordata Thunb Ethanol Extracts Increases Tumor Infiltrating CD8+/Treg Cells Ratio and Inhibits Cutaneous Squamous Cell Carcinoma In Vivo

10.21203/rs.3.rs-1170208/v1 ◽

2021 ◽

Author(s):

Lipeng Gao ◽

Rong-Yin Gui ◽

Xin-Nan Zheng ◽

Ying-Xue Wang ◽

Yao Gong ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Treg Cells ◽

Cutaneous Squamous Cell Carcinoma ◽

Therapeutic Effects ◽

Houttuynia Cordata ◽

Anti Cancer ◽

Houttuynia Cordata Thunb

Abstract Houttuynia cordata Thunb (HCT) is a medicinal and edible herb which has beneficial effects on various diseases due to its diuretic, anti-inflammatory, anti-oxidative, anti-microbial, anti-viral, anti-cancer and anti-diabetic properties. Most of reports of its anti-cancer activity were conducted in vitro, and its effects on cutaneous squamous cell carcinoma (SCC) has not been investigated yet. Using DMBA/TPA induced SCC mice model, we found that topical treatment by HCT, as well as its bioactive ingredient monomer, efficiently inhibited tumor growth. Mechanistically, we found tumor infiltrating CD4+, Foxp3+ T regulatory cells (Tregs) were significantly reduced and CD8+/Treg cells ratio was largely increased in tumors after HCT treatment. In addition, several chemokines which recruited immune cells were largely reduced when SCC cancer cells were treated by HCT in vitro. Our results demonstrate the therapeutic effects of HCT on cutaneous SCC and indicate it might inhibit cancer through regulating tumor infiltrating lymphocytes and the tumor immune microenvironments.

Download Full-text

USF1-induced upregulation of LINC01048 promotes cell proliferation and apoptosis in cutaneous squamous cell carcinoma by binding to TAF15 to transcriptionally activate YAP1

Cell Death and Disease ◽

10.1038/s41419-019-1516-2 ◽

2019 ◽

Vol 10 (4) ◽

Cited By ~ 12

Author(s):

Lezi Chen ◽

Quan Chen ◽

Shifeng Kuang ◽

Chengli Zhao ◽

Lu Yang ◽

...

Keyword(s):

Cell Proliferation ◽

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma ◽

Proliferation And Apoptosis

Download Full-text

Clinicopathological roles of S100A8 and S100A9 in cutaneous squamous cell carcinoma in vivo and in vitro

Archives of Dermatological Research ◽

10.1007/s00403-014-1453-y ◽

2014 ◽

Vol 306 (5) ◽

pp. 489-496 ◽

Cited By ~ 15

Author(s):

Dae-Kyoung Choi ◽

Zheng Jun Li ◽

In-Kyu Chang ◽

Min-Kyung Yeo ◽

Jin-Man Kim ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cutaneous Squamous Cell Carcinoma

Download Full-text

miR-22 promotes stem cell traits via activating Wnt/β-catenin signaling in cutaneous squamous cell carcinoma

Oncogene ◽

10.1038/s41388-021-01973-5 ◽

2021 ◽

Author(s):

Shukai Yuan ◽

Peitao Zhang ◽

Liqi Wen ◽

Shikai Jia ◽

Yufan Wu ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Cancer Metastasis ◽

Cutaneous Squamous Cell Carcinoma ◽

Chemotherapy Sensitivity ◽

Oncogenic Pathways ◽

Chemically Induced ◽

Xenograft Mice

AbstractEmerging evidence suggests that the cancer stem cells (CSCs) are key culprits of cancer metastasis and drug resistance. Understanding mechanisms regulating the critical oncogenic pathways and CSCs function could reveal new diagnostic and therapeutic strategies. We now report that miR-22, a miRNA critical for hair follicle stem/progenitor cell differentiation, promotes tumor initiation, progression, and metastasis by maintaining Wnt/β-catenin signaling and CSCs function. Mechanistically, we find that miR-22 facilitates β-catenin stabilization through directly repressing citrullinase PAD2. Moreover, miR-22 also relieves DKK1-mediated repression of Wnt/β-catenin signaling by targeting a FosB-DDK1 transcriptional axis. miR-22 knockout mice showed attenuated Wnt/β-catenin activity and Lgr5+ CSCs penetrance, resulting in reduced occurrence, progression, and metastasis of chemically induced cutaneous squamous cell carcinoma (cSCC). Clinically, miR-22 is abundantly expressed in human cSCC. Its expression is even further elevated in the CSCs proportion, which negatively correlates with PAD2 and FosB expression. Inhibition of miR-22 markedly suppressed cSCC progression and increased chemotherapy sensitivity in vitro and in xenograft mice. Together, our results revealed a novel miR-22-WNT-CSCs regulatory mechanism in cSCC and highlight the important clinical application prospects of miR-22, a common target molecule for Wnt/β-catenin signaling and CSCs, for patient stratification and therapeutic intervention.

Download Full-text