scholarly journals Anti-inflammatory effect of bee venom in an allergic chronic rhinosinusitis mouse model

2018 ◽  
Author(s):  
Seung‑Heon Shin ◽  
Mi‑Kyung Ye ◽  
Sung‑Yong Choi ◽  
Kwan‑Kyu Park
Keyword(s):  
Mouse Model ◽  
Chronic Rhinosinusitis ◽  
Bee Venom ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals TAK1/AP-1-Targeted Anti-Inflammatory Effects of Barringtonia augusta Methanol Extract

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 3053
Author(s):  
Anh Thu Ha ◽  
Mi-Yeon Kim ◽  
Jae Youl Cho
Keyword(s):  
Mouse Model ◽  
Methanol Extract ◽  
Folk Medicine ◽  
Activator Protein 1 ◽  
Western Blot Assay ◽  
In Vivo Experiments ◽  
Chemokine Ligand ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Barringtonia augusta methanol extract (Ba-ME) is a folk medicine found in the wetlands of Thailand that acts through an anti-inflammatory mechanism that is not understood fully. Here, we examine how the methanol extract of Barringtonia augusta (B. augusta) can suppress the activator protein 1 (AP-1) signaling pathway and study the activities of Ba-ME in the lipopolysaccharide (LPS)-treated RAW264.7 macrophage cell line and an LPS-induced peritonitis mouse model. Non-toxic concentrations of Ba-ME downregulated the mRNA expression of cytokines, such as cyclooxygenase and chemokine ligand 12, in LPS-stimulated RAW264.7 cells. Transfection experiments with the AP-1-Luc construct, HEK293T cells, and luciferase assays were used to assess whether Ba-ME suppressed the AP-1 functional activation. A Western blot assay confirmed that C-Jun N-terminal kinase is a direct pharmacological target of Ba-ME action. The anti-inflammatory effect of Ba-ME, which functions by β-activated kinase 1 (TAK1) inhibition, was confirmed by using an overexpression strategy and a cellular thermal shift assay. In vivo experiments in a mouse model of LPS-induced peritonitis showed the anti-inflammatory effect of Ba-ME on LPS-stimulated macrophages and acute inflammatory mouse models. We conclude that Ba-ME is a promising anti-inflammatory drug targeting TAK1 in the AP-1 pathway.

41. Anti-Inflammatory Effect of Honey Bee Venom on Wistar Rats Induced Poly Cystic Ovarian Syndrome by Estradiol Valerate

Toxicon ◽  
2012 ◽  
Vol 60 (2) ◽  
pp. 115-116
Author(s):  
Mohammad Nabiuni ◽  
Kazem Parivar ◽  
Bahman Zeynali ◽  
Azar Sheikholeslami ◽  
Latifeh Karimzadeh
Keyword(s):  
Honey Bee ◽  
Wistar Rats ◽  
Bee Venom ◽  
Estradiol Valerate ◽  
Anti Inflammatory ◽  
Ovarian Syndrome ◽  
Inflammatory Effect

scholarly journals Effect of Bee Venom Pharmacopuncture on Inflammation in Mouse Model of Induced Atopic Dermatitis

2020 ◽  
Vol 37 (2) ◽  
pp. 123-127
Author(s):  
Kyeong Ju Park ◽  
Ho-Sueb Song
Keyword(s):  
Atopic Dermatitis ◽  
Mouse Model ◽  
Treated Group ◽  
Bee Venom ◽  
Related Protein ◽  
Griess Reaction ◽  
Cox 2 ◽  
Dose Dependent Decrease ◽  
Dose Dependent ◽  
Inflammatory Effect

Background: This study was designed using a mouse model of atopic dermatitis [phthalic anhydride (PA)-treated mice], to investigate the anti-inflammatory effect of bee venom pharmacopuncture (BVP) in keratinocytes.Methods: Western blot analysis was performed to investigate inflammation related protein expression of iNOS, COX-2, phospho-ERK (p-ERK), and ERK, in LPS (1 μg/mL)-activated keratinocytes, following BVP treatment, and in PA-treated mice, after BVP treatment. Griess reaction was performed to investigate NO concentration. Enzyme-linked immunosorbent assays were used to determine the concentrations of interleukin (IL)-4+, IL-17A+, IL-13 and IL-4 in PA-treated mice after BVP treatment. In addition, monocyte, macrophage, neutrophil, and eosinophil counts were measured to observe the changes in white blood cell infiltration.Results: The keratinocytes of the BVP-treated group showed a decreased expression of iNOS, COX-2, ERK at 5 OX-2, ERK E, and p-ERK at 1, 2 and 5 RKRK ERK ERK, and a dose-dependent decrease in NO concentration at 2 and 5 ntrationof s. In the BVP-treated groups (0.1 μ.1-trea μ.1-treated gr), PA-treated mice showed recovery after 4 weeks which was dose-dependent, showing a significant decrease in clinical scores for AD, and a decreased concentration of IL-13 and IL-4 with BV treatment. There was a dose-dependent decrease in the infiltration of eosinophils, neutrophils, monocytes, macrophages, and a decreased thickness of the epidermis due to inflammation, and decreased expressions of iNOS, COX-2, p-ERK, ERK, especially in the 0.1 μ0/mL BVP-treated group,<br>Conclusion: These results suggest that BVP may be an effective alternative treatment for atopic dermatitis.

Anti-inflammatory Effect of Arginase Inhibitor and Corticosteroid on Airway Allergic Reactions in a Dermatophogoides farinae-induced NC/Nga Mouse Model

Inflammation ◽  
2012 ◽  
Vol 36 (1) ◽  
pp. 141-151 ◽  
Author(s):  
Keiki Ogino ◽  
Masayuki Kubo ◽  
Hidekazu Takahashi ◽  
Ran Zhang ◽  
Yu Zou ◽  
...  
Keyword(s):  
Mouse Model ◽  
Allergic Reactions ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals The Proteolytic Fraction From Vasconcellea cundinamarcensis Latex Displays Anti-Inflammatory Effect in A Mouse Model of Acute TNBS-Induced Colitis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ronniel Morais Albuquerque ◽  
Marina Passos Pizzitola ◽  
Ana Cândida Araújo e Silva ◽  
Dalton Dittz ◽  
Kátia Michelle Freitas ◽  
...  
Keyword(s):  
Mouse Model ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Bee Venom Pretreatment Has Both an Antinociceptive and Anti-Inflammatory Effect on Carrageenan-Induced Inflammation.

2001 ◽  
Vol 63 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Jang-Hern LEE ◽  
Young-Bae KWON ◽  
Ho-Jae HAN ◽  
Woung-Chon MAR ◽  
Hye-Jung LEE ◽  
...  
Keyword(s):  
Bee Venom ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals IFN-τDisplays Anti-Inflammatory Effects onStaphylococcus aureusEndometritis via Inhibiting the Activation of the NF-κB and MAPK Pathways in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-12
Author(s):  
Zhenbiao Zhang ◽  
Yingfang Guo ◽  
Yuzhu Liu ◽  
Chengye Li ◽  
Mengyao Guo ◽  
...  
Keyword(s):  
Mouse Model ◽  
Protective Effect ◽  
Inflammatory Cytokine ◽  
Inflammatory Diseases ◽  
Potential Drug ◽  
Histopathological Changes ◽  
Uterine Infection ◽  
Anti Inflammatory ◽  
Anti Inflammatory Cytokine ◽  
Inflammatory Effect

The aim of the present study was to determine the anti-inflammatory effect of IFN-τon endometritis using a mouse model ofS. aureus-induced endometritis and to elucidate the mechanism of action underlying these effects. In the present study, the effect of IFN-τonS. aureusgrowth was monitored by turbidimeter at 600 nm. IFN-τdid not affectS. aureusgrowth. The histopathological changes indicated that IFN-τhad a protective effect on uterus tissues withS. aureusinfection. The ELISA and qPCR results showed the production of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 was decreased with IFN-τtreatment. In contrast, the level of the anti-inflammatory cytokine IL-10 was increased. We further studied the signaling pathway associated with these observations, and the qPCR results showed that the expression of TLR2 was repressed by IFN-τ. Furthermore, the western blotting results showed the phosphorylation of IκB, NF-κB p65, and MAPKs (p38, JNK, and ERK) was inhibited by IFN-τtreatment. The results suggested that IFN-τmay be a potential drug for the treatment of uterine infection due toS. aureusor other infectious inflammatory diseases.

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