Diallyl sulfide protects against dilated cardiomyopathy via inhibition of oxidative stress and apoptosis in mice

Diallyl sulfide, a sulfur-containing volatile compound present in garlic ( Allium sativum), exerts anticarcinogenic activity in various rodent tumor models. In the present study, apoptosis-inhibiting effects of diallyl sulfide against a carcinogenic polycyclic aromatic hydrocarbon, 7,12-dimethyl benz(a)anthracene (DMBA), in Swiss albino mice were observed. The animals were given either 250 μg/mouse or 500 μg/mouse of diallyl sulfide for 1 week after a single intragastric dose of 7,12-dimethyl benz(a)anthracene (50 mg/kg body weight). Results showed that diallyl sulfide supplementation effectively protects against 7,12-dimethyl benz(a)anthracene—induced oxidative stress, characterized by restored antioxidant enzyme levels (up to 64%) and lipid peroxidation (up to 25%). Flow cytometric analysis showed a reduction in apoptotic cell population in hypodiploid region in diallyl sulfide–supplemented animals. Inhibition of apoptosis was preceded by decrease in reactive oxygen species levels and restoration of mitochondrial transmembrane potential followed by decreased DNA fragmentation. In 7,12-dimethyl benz(a)anthracene–exposed animals, downregulation (~30%) of antiapoptotic Bcl-2 and upregulation (~60%) of pro-apoptotic Bax proteins were observed. These alterations were restored significantly by diallyl sulfide supplementation, indicating inhibition of apoptosis. Thus, these results show that diallyl sulfide provides protection against oxidative damage induced by 7,12-dimethyl benz(a)anthracene in mouse liver and may be an effective chemopreventive and therapeutic agent by modulating expression of cell-growth regulatory proteins.

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Decreased oxidative stress in patients with idiopathic dilated cardiomyopathy one year after immunoglobulin adsorption

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(01)01309-2 ◽

2001 ◽

Vol 38 (1) ◽

pp. 178-183 ◽

Cited By ~ 42

Author(s):

Ingolf Schimke ◽

Johannes Müller ◽

Friedrich Priem ◽

Ingrid Kruse ◽

Birgit Schön ◽

...

Keyword(s):

Oxidative Stress ◽

Dilated Cardiomyopathy ◽

Idiopathic Dilated Cardiomyopathy ◽

One Year

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The Impact of Myocardial Oxidative Stress on LV Remodeling in Patients with Dilated Cardiomyopathy

Journal of Cardiac Failure ◽

10.1016/j.cardfail.2011.06.644 ◽

2011 ◽

Vol 17 (9) ◽

pp. S143

Author(s):

Wakako Murakami ◽

Shigeki Kobayashi ◽

Takehisa Susa ◽

Yasuaki Wada ◽

Jutaro Yamada ◽

...

Keyword(s):

Oxidative Stress ◽

Dilated Cardiomyopathy ◽

Myocardial Oxidative Stress ◽

Lv Remodeling ◽

The Impact

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Oxidative Stress Markers and C-Reactive Protein Are Related to Severity of Heart Failure in Patients with Dilated Cardiomyopathy

Mediators of Inflammation ◽

10.1155/2014/147040 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 19

Author(s):

Celina Wojciechowska ◽

Ewa Romuk ◽

Andrzej Tomasik ◽

Bronisława Skrzep-Poloczek ◽

Ewa Nowalany-Kozielska ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Dilated Cardiomyopathy ◽

Nyha Class ◽

Right Heart Catheterization ◽

Heart Catheterization ◽

Hemodynamic Parameters ◽

C Reactive Protein ◽

Reactive Protein ◽

Hs Crp

Background.The aim of study was to determine relationships between functional capacity (NYHA class), left ventricle ejection fraction (LVEF), hemodynamic parameters, and biomarkers of redox state and inflammation in patients with dilated cardiomyopathy (DCM).Methods. DCM patients (n=109, aged45.97±10.82years), NYHA class IIV, and LVEF2.94±7.1% were studied. Controls comprised age-matched healthy volunteers (n=28). Echocardiography and right heart catheterization were performed. Serum activities of superoxide dismutase isoenzymes (MnSOD and CuZnSOD), concentrations of uric acid (UA), malondialdehyde (MDA), and C-reactive protein (hs-CRP) were measured.Results. MnSOD, UA, hs-CRP, and MDA were significantly higher in DCM patients compared to controls. Except MDA concentration, above parameters were higher in patients in III-IV NYHA class or with lower LVEF. hsCRP correlated with of MnSOD (P<0.05) and CuZnSOD activity (P<0.01). Both isoenzymes positively correlated with mPAP and pulmonary capillary wedge pressure (MnSOD, resp.,P<0.01andP<0.05and CuZnSODP<0.05;P<0.05). UA positively correlated with MnSOD (P<0.05), mPAP (P<0.05), and PVRI (P<0.05). The negative correlation between LVEF and UA (P<0.01) was detected.Conclusion. There are relationships among the severity of symptoms of heart failure, echocardiographic hemodynamic parameters, oxidative stress, and inflammatory activation. Increased MnSOD activity indicates the mitochondrial source of ROS in patients with advanced heart failure.

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