Effects of dexmedetomidine on inflammatory factors, T lymphocyte subsets and expression of NF‑κB in peripheral blood mononuclear cells in patients receiving radical surgery of colon carcinoma

The spontaneous proliferation of peripheral blood mononuclear cells (PBMCs) is a hallmark of the human T-lymphotropic virus (HTLV) type-1. Cell proliferation is usually measured using a [3H]thymidine incorporation assay. This study aims to quantify the spontaneous proliferation of PBMCs using flow cytometry. PBMCs were cultured for 24 to 120 hours in the presence of 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE). For comparison, PBMCs were also cultured with [3H]thymidine. The cutoff values for spontaneous proliferation were >0.06 for the division index and >5.8% for the percentage of divided cells. Sixty-two percent of HTLV-1-infected individuals presented spontaneous proliferation of PBMCs, which was detected in the first 24 hours. Moreover, proliferation was detected in CD4+ and CD8+ T-lymphocyte subsets. A positive correlation was found between the division index and [3H]thymidine incorporation. This method may prove useful to better understand the phenomenon of spontaneous proliferation of PBMC of patients infected with HTLV-1.

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Nutritionally Mediated Oxidative Stress and Inflammation

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/610950 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 82

Author(s):

Alexandra Muñoz ◽

Max Costa

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Arachidonic Acid ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Saturated Fatty Acids ◽

Inflammatory Factors ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

There are many sources of nutritionally mediated oxidative stress that trigger inflammatory cascades along short and long time frames. These events are primarily mediated via NFκB. On the short-term scale postprandial inflammation is characterized by an increase in circulating levels of IL-6 and TNF-αand is mirrored on the long-term by proinflammatory gene expression changes in the adipocytes and peripheral blood mononuclear cells (PBMCs) of obese individuals. Specifically the upregulation ofCCL2/MCP-1,CCL3/MIP-1α,CCL4/MIP-1β,CXCL2/MIP-2α, andCXCL3/MIP-2βis noted because these changes have been observed in both adipocytes and PBMC of obese humans. In comparing numerous human intervention studies it is clear that pro-inflammatory and anti-inflammatory consumption choices mediate gene expression in humans adipocytes and peripheral blood mononuclear cells. Arachidonic acid and saturated fatty acids (SFAs) both demonstrate an ability to increase pro-inflammatory IL-8 along with numerous other inflammatory factors including IL-6, TNFα, IL-1β, and CXCL1 for arachidonic acid and IGB2 and CTSS for SFA. Antioxidant rich foods including olive oil, fruits, and vegetables all demonstrate an ability to lower levels of IL-6 in PBMCs. Thus, dietary choices play a complex role in the mediation of unavoidable oxidative stress and can serve to exacerbate or dampen the level of inflammation.

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Association between T lymphocyte sub-sets apoptosis and peripheral blood mononuclear cells oxidative stress in systemic lupus erythematosus

Free Radical Research ◽

10.3109/10715762.2011.555765 ◽

2011 ◽

Vol 45 (5) ◽

pp. 559-567 ◽

Cited By ~ 39

Author(s):

Dilip Shah ◽

Ashish Aggarwal ◽

Archana Bhatnagar ◽

Ravi Kiran ◽

Ajay Wanchu

Keyword(s):

Oxidative Stress ◽

Systemic Lupus Erythematosus ◽

T Lymphocyte ◽

Peripheral Blood Mononuclear Cells ◽

Lupus Erythematosus ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Systemic Lupus ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Activation of Human Peripheral Blood Mononuclear Cells by Nonpathogenic Bacteria In Vitro: Evidence of NK Cells as Primary Targets

Infection and Immunity ◽

10.1128/iai.68.2.752-759.2000 ◽

2000 ◽

Vol 68 (2) ◽

pp. 752-759 ◽

Cited By ~ 131

Author(s):

D. Haller ◽

S. Blum ◽

C. Bode ◽

W. P. Hammes ◽

E. J. Schiffrin

Keyword(s):

Nk Cells ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Lymphocyte Subsets ◽

Human Peripheral Blood ◽

Peripheral Blood Mononuclear ◽

E Coli ◽

Blood Mononuclear Cells ◽

Ifn Γ

ABSTRACT The interaction of commensal bacteria with immunocompetent cells may occur in definite compartments of the mucosal immune system, as limited translocation through the epithelial barrier cannot be excluded. In this study the stimulation of human peripheral blood mononuclear cells and purified lymphocyte subsets by nonpathogenic gram-positive lactobacilli (Lactobacillus johnsonii andLactobacillus sakei) and gram-negative Escherichia coli was investigated. The various bacterial strains induced a differential cytokine pattern. Whereas L. johnsonii andL. sakei strongly induced gamma interferon (IFN-γ) and interleukin-12 (IL-12), E. coli and lipopolysaccharide (LPS) preferentially induced IL-10 after 16 h of stimulation. Expression of activation antigens CD69 and CD25 was observed on (CD3− CD56+) natural killer (NK) cells after stimulation of total human peripheral blood mononuclear cells. All bacteria mediated the proliferation of human peripheral blood mononuclear cells, and the strongest proliferative response was observed with L. johnsonii. Purified CD4+, CD8+, and CD19+ lymphocyte subsets were not activated upon bacterial stimulation but showed normal response to a mitogenic stimulus. In contrast, purified NK cells upregulated the IL-2Rα chain (CD25) and underwent proliferation when stimulated byL. johnsonii. E. coli and LPS were less effective in inducing proliferation. Expression of CD25 or secretion of IFN-γ from purified NK cells was significantly increased in the presence of bacterially primed macrophages, indicating that full activation required both bacterium- and cell contact-based signals derived from accessory cells.

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B-Lymphocyte Proliferation during Bovine Leukemia VirusInduced Persistent Lymphocytosis Is Enhanced by T-Lymphocyte-Derived Interleukin-2

Journal of Virology ◽

10.1128/jvi.72.4.3169-3177.1998 ◽

1998 ◽

Vol 72 (4) ◽

pp. 3169-3177 ◽

Cited By ~ 26

Author(s):

Esther S. Trueblood ◽

Wendy C. Brown ◽

Guy H. Palmer ◽

William C. Davis ◽

Diana M. Stone ◽

...

Keyword(s):

B Lymphocytes ◽

T Lymphocyte ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

B Lymphocyte ◽

Mononuclear Cells ◽

Interleukin 2 ◽

Persistent Lymphocytosis ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

ABSTRACT Bovine leukemia virus (BLV)-induced persistent lymphocytosis is characterized by a polyclonal expansion of CD5+ B lymphocytes. To examine the role of the cytokine microenvironment in this virus-induced B-lymphocyte expansion, the expression of interleukin-2 (IL-2), IL-4, IL-10, and gamma interferon (IFN-γ) mRNA, was measured in stimulated peripheral blood mononuclear cells from persistently lymphocytotic BLV-infected cows, nonlymphocytotic BLV-infected cows, and uninfected cows. IL-2 and IL-10 mRNA expression and IL-2 functional activity were significantly increased when peripheral blood mononuclear cells from persistently lymphocytotic cows were stimulated with concanavalin A (ConA). Additionally, during persistent lymphocytosis, peak IL-2 and IL-10 mRNA expression was delayed, and elevated expression was prolonged. To determine the potential biologic importance of increased IL-2 and IL-10 expression, the response of isolated B lymphocytes from persistently lymphocytotic cows to human recombinant cytokines and to cytokine-containing supernatants from isolated T lymphocytes was examined. While recombinant human IL-10 (rhIL-10) did not consistently induce detectable changes, rhIL-2 increased viral protein (p24) and IL-2 receptor expression in isolated B lymphocytes from persistently lymphocytotic cows. Additionally, rhIL-2 and supernatant from ConA-stimulated T lymphocytes enhanced B-lymphocyte proliferation. The stimulatory activity of the T-lymphocyte supernatant could be completely inhibited with a polyclonal anti-rhIL-2 antibody. Finally, polyclonal anti-rhIL-2 antibody, as well as anti-BLV antibody, inhibited spontaneous proliferation of peripheral blood mononuclear cells from persistently lymphocytotic cows, demonstrating that the spontaneous lymphoproliferation characteristic of BLV-induced persistent lymphocytosis is IL-2 dependent and antigen dependent. Collectively, these findings strongly suggest that increased T-lymphocyte expression of IL-2 in BLV-infected cows contributes to development and/or maintenance of persistent B lymphocytosis.

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