Measurements of cellular proliferation by H-3-thymidine-labeling index and flow cytometry in breast cancer patients

1997 ◽  
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S Cascinu ◽  
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Oncology ◽  
2000 ◽  
Vol 60 (1) ◽  
pp. 88-93 ◽  
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Paola Queirolo ◽  
Stefania Vecchio ◽  
Rita Lionetto ◽  
Lucia Del Mastro ◽  
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1985 ◽  
Vol 5 (2) ◽  
pp. 117-128 ◽  
Author(s):  
William L McGuire ◽  
John S Meyer ◽  
Barthel Barlogie ◽  
Timothy E Kute

Breast Cancer ◽  
2012 ◽  
Vol 21 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Kentaro Tamaki ◽  
Takanori Ishida ◽  
Nobumitsu Tamaki ◽  
Yoshihiko Kamada ◽  
Kanou Uehara ◽  
...  

Cancer ◽  
1990 ◽  
Vol 66 (8) ◽  
pp. 1810-1816 ◽  
Author(s):  
T. E. Kute ◽  
H. B. Muss ◽  
M. R. Cooper ◽  
L. D. Case ◽  
D. Buss ◽  
...  

2021 ◽  
Vol 22 (5) ◽  
pp. 2504
Author(s):  
Liubov A. Tashireva ◽  
Olga E. Savelieva ◽  
Evgeniya S. Grigoryeva ◽  
Yuri V. Nikitin ◽  
Evgeny V. Denisov ◽  
...  

To date, there is indisputable evidence of significant CTC heterogeneity in carcinomas, in particular breast cancer. The heterogeneity of CTCs is manifested in the key characteristics of tumor cells related to metastatic progression – stemness and epithelial–mesenchymal (EMT) plasticity. It is still not clear what markers can characterize the phenomenon of EMT plasticity in the range from epithelial to mesenchymal phenotypes. In this article we examine the manifestations of EMT plasticity in the CTCs in breast cancer. The prospective study included 39 patients with invasive carcinoma of no special type. CTC phenotypes were determined by flow cytometry before any type of treatment. EMT features of CTC were assessed using antibodies against CD45, CD326 (EpCam), CD325 (N-cadherin), CK7, Snail, and Vimentin. Circulating tumor cells in breast cancer are characterized by pronounced heterogeneity of EMT manifestations. The results of the study indicate that the majority of heterogeneous CTC phenotypes (22 out of 24 detectable) exhibit epithelial–mesenchymal plasticity. The variability of EMT manifestations does not prevent intravasation. Co-expression of EpCAM and CK7, regardless of the variant of co-expression of Snail, N-cadherin, and Vimentin, are associated with a low number of CTCs. Intrapersonal heterogeneity is manifested by the detection of several CTC phenotypes in each patient. Interpersonal heterogeneity is manifested by various combinations of CTC phenotypes in patients (from 1 to 17 phenotypes).


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