scholarly journals Species delimitation of Margattea cockroaches from China, with seven new species (Blattodea, Ectobiidae, Pseudophyllodromiinae)

ZooKeys ◽  
2021 ◽  
Vol 1036 ◽  
pp. 121-151
Author(s):  
Jia-Jun He ◽  
Du-Ting Jin ◽  
Yi-Shu Wang ◽  
Yan-Li Che ◽  
Zong-Qing Wang
Keyword(s):  
New Species ◽  
Confidence Interval ◽  
Genetic Distance ◽  
Species Delimitation ◽  
Phylogenetic Analyses ◽  
Operational Taxonomic Units ◽  
As Species ◽  
The Difference ◽  
Morphological Differences ◽  
Barcode Gap

Nearly 450 Margattea specimens were collected from 27 locations in China and their morphology was examined. Then 68 Margattea COI sequences were obtained and used to carry out phylogenetic analyses as well as species delimitation analyses using General Mixed Yule Coalescent (GMYC), Automatic Barcode Gap Discovery (ABGD), and Poisson Tree Processes (bPTP). GMYC analysis resulted in 21 molecular operational taxonomic units (MOTUs) (confidence interval: 20–22), which was completely consistent with the result of the bPTP. There were 15 MOTUs using the ABGD method. The number of MOTUs was slightly different from the assigned morphospecies (16). As to the incongruence between molecular and morphological results, we checked the specimens again and made sure that most morphological differences were determined to be intraspecific differences (except the difference between M. angusta and M. mckittrickae), although a large genetic distance existed. Finally, 16 Margattea species from China were defined in this study, of which, seven new species are established, i.e. Margattea deltodonta J-J He & Z-Q Wang, sp. nov., Margattea cuspidata J-J He & Z-Q Wang, sp. nov., Margattea caudata J-J He & Z-Q Wang, sp. nov., Margattea paratransversa J-J He & Z-Q Wang, sp. nov., Margattea disparilis J-J He & Z-Q Wang, sp. nov., Margattea transversa J-J He & Z-Q Wang, sp. nov., and Margattea bicruris J-J He & Z-Q Wang, sp. nov.

Two new species of Hemiphyllodactylus (Squamata: Gekkonidae) from Hoa Binh Province, Vietnam

Zootaxa ◽  
2020 ◽  
Vol 4801 (3) ◽  
pp. 513-536
Author(s):  
TRUONG QUANG NGUYEN ◽  
QUYEN HANH DO ◽  
HANH THI NGO ◽  
ANH VAN PHAM ◽  
CUONG THE PHAM ◽  
...  
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Nature Reserve ◽  
Phylogenetic Analyses ◽  
Color Pattern ◽  
Karst Forest ◽  
Two New Species ◽  
Morphological Differences ◽  
Nd2 Gene ◽  
Hoa Binh

We describe two new species of the genus Hemiphyllodactylus on the basis of a new collection of geckos from limestone karst forest of Hoa Binh Province, northwestern Vietnam. Hemiphyllodactylus bonkowskii sp. nov. from Hang Kia—Pa Co Nature Reserve and Hemiphyllodactylus ngocsonensis sp. nov. from Ngoc Son—Ngo Luong Nature Reserve can be distinguished from each other and from their congeners by genetic distinction and morphological differences in circumnasal scales, chin scales, precloacal and femoral pores, cloacal spurs, dorsal scales, ventral scales, digital lamellae formula, and color pattern. In phylogenetic analyses, both new species are nested in a clade that includes H. dushanensis, H. flaviventris, H. hongkongensis, H. huishuiensis, H. kiziriani, H. serpispecus, and H. zugi. In terms of pairwise genetic distance, the two new species are at least 7.5%–8.5% divergent from other described species based on a fragment of the ND2 gene. 

A new species of Hemiphyllodactylus (Squamata: Gekkonidae) from Tuyen Quang Province, Vietnam

Zootaxa ◽  
2020 ◽  
Vol 4821 (3) ◽  
pp. 511-532
Author(s):  
QUYEN HANH DO ◽  
CUONG THE PHAM ◽  
TIEN QUANG PHAN ◽  
MINH DUC LE ◽  
THOMAS ZIEGLER ◽  
...  
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Unique Combination ◽  
Northern Vietnam ◽  
Karst Forest ◽  
Morphological Differences ◽  
A New Species ◽  
Lateral Head

A new species of the gekkonid genus Hemiphyllodactylus is described from limestone karst forest of Tuyen Quang Province, northern Vietnam based on morphological differences and molecular divergence. Hemiphyllodactylus nahangensis sp. nov. is distinguished from the remaining congeners by having the unique combination of the following characters: a bisexual taxon; SVL of adults 41.4–43.6 mm; dorsal scale rows 18–23; ventral scale rows 9–13; chin scales bordering mental and first infralabial distinctly enlarged; 22–24 pore-bearing femoral and precloacal scales, in a continuous row, absent in females; digital lamella formula 3-4-5-4 (forefoot) and 4-5-5-5 (hindfoot); cloacal spur single, present in both sexes; dark lateral head stripe indistinct; postsacral mark cream and bearing anteriorly projecting arms. In phylogenetic analyses, the new species is placed in a clade that includes H. dushanensis, H. hongkongensis, H. huishuiensis, H. ngocsonensis and H. zugi. In terms of pairwise genetic distance, the new species is at least 5.9%–6.6% divergent from other congeners based on a fragment of the mitochondrial gene ND2.

A new species of Cyrtodactylus (Squamata: Gekkonidae) from Ninh Thuan Province, southern Vietnam

Zootaxa ◽  
2021 ◽  
Vol 4999 (1) ◽  
pp. 58-76
Author(s):  
Quyen Hanh Do ◽  
TRUNG MY PHUNG ◽  
HANH THI NGO ◽  
MINH DUC LE ◽  
THOMAS ZIEGLER ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Coi Gene ◽  
Unique Combination ◽  
Mitochondrial Coi ◽  
Southern Vietnam ◽  
Mitochondrial Coi Gene ◽  
Morphological Differences ◽  
Dorsal Pattern ◽  
A New Species

A new species of the Cyrtodactylus irregularis group is described from Ninh Thuan Province, southern Vietnam based on molecular divergence and morphological differences. Cyrtodactylus orlovi sp. nov. is distinguished from the remaining Indochinese bent-toed geckos by having the unique combination of the following characters: size medium (SVL 61.0–77.7 mm); dorsal tubercles in 16–20 irregular rows; 36–39 ventral scale rows; precloacal pores absent in females, 5 or 6 in males, in a continuous row; femoral pores absent; 3–8 enlarged femoral scales; postcloacal spurs 1 or 2; lamellae under toe IV 16–19; a continuous neckband; a highly irregular transverse banded dorsal pattern; the absence of transversely enlarged median subcaudal scales. In phylogenetic analyses, the new species was revealed to be the sister taxon to a clade consisting of Cyrtodactylus cattienensis and the most recently described species from Vietnam, C. chungi, with 12.1–12.4% and 11.7 % pairwise genetic divergence from the two species, respectively, based on a fragment of the mitochondrial COI gene.  

A new species of Psammophis (Serpentes: Psammophiidae) from the Turpan Basin in northwest China

Zootaxa ◽  
2021 ◽  
Vol 4974 (1) ◽  
pp. 116-134
Author(s):  
MINLI CHEN ◽  
JINLONG LIU ◽  
BO CAI ◽  
JUN LI ◽  
NA WU ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Northwest China ◽  
Genetic Differences ◽  
Cytb Gene ◽  
Turpan Basin ◽  
Morphological Differences ◽  
A New Species

An adult sand snake specimen was collected during a herpetofaunal survey conducted in the Turpan Basin in northwest China. Phylogenetic analyses revealed that this specimen, along with other snake sloughs and skins collected from different localities in the Turpan Basin formed a clade that is sister to Psammophis lineolatus. This taxon exhibited substantial divergence from its congeners (P. lineolatus and P. condanarus) with uncorrelated p-distances ranging from 11.9 ± 0.9% to 15.8 ± 1.6% for the ND4 gene and from 10.2 ± 0.8% to 13.8 ± 1.1% for the Cytb gene. Given the genetic differences along with morphological differences, we describe the specimen from the Turpan Basin as Psammophis turpanensis sp. nov. We provide detailed morphological descriptions, and compare this specimen with five Asian sand snakes and the Afro-Asian Sand Snake, P. schokari. In addition, we provide brief comments on the biogeography of Psammophis in China. 

Molecular systematics of Thouarella (Octocorallia:Primnoidae) with the description of three new species from the Southern Ocean based on combined molecular and morphological evidence

2021 ◽  
Author(s):  
Mónica Núñez-Flores ◽  
Daniel Gomez-Uchida ◽  
Pablo J. López-González
Keyword(s):  
New Species ◽  
Southern Ocean ◽  
Species Delimitation ◽  
Nuclear Dna ◽  
Phylogenetic Analyses ◽  
Weddell Sea ◽  
Morphological Evidence ◽  
Three New Species ◽  
Taxonomic Framework ◽  
The Antarctic

Thouarella Gray, 1870, is one of the most speciose genera among gorgonians of the family Primnoidae (Cnidaria:Octocorallia:Anthozoa), being remarkably diverse in the Antarctic and sub-Antarctic seafloor. However, their diversity in the Southern Ocean is likely underestimated. Phylogenetic analyses of mitochondrial and nuclear DNA markers were integrated with species delimitation approaches as well as morphological colonial and polyps features and skeletal SEM examinations to describe and illustrate three new species within Thouarella, from the Weddell Sea, Southern Ocean: T. amundseni sp. nov., T. dolichoespinosa sp. nov. and T. pseudoislai sp. nov. Our species delimitation results suggest, for the first time, the potential presence of Antarctic and sub-Antarctic cryptic species of primnoids, based on the likely presence of sibling species within T. undulata and T. crenelata. With the three new species here described, the global diversity of Thouarella has increased to 41 species, 15 of which are endemic to the Antarctic and sub-Antarctic waters. Consequently, our results provide new steps for uncovering the shelf benthonic macrofauna’s hidden diversity in the Southern Ocean. Finally, we recommend using an integrative taxonomic framework in this group of organisms and species delimitation approaches because the distinctions between some Thouarella species based only on a superficial examination of their macro- and micromorphological features is, in many cases, limited.

The green puzzle Stichococcus (Trebouxiophyceae, Chlorophyta): New generic and species concept among this widely distributed genus

Phytotaxa ◽  
2020 ◽  
Vol 441 (2) ◽  
pp. 113-142 ◽  
Author(s):  
THOMAS PRÖSCHOLD ◽  
TATYANA DARIENKO
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Species Concept ◽  
Phylogenetic Analyses ◽  
Complex Life Cycle ◽  
New Genera ◽  
Rdna Sequences ◽  
Taxonomic History ◽  
Culture Independent ◽  
Science Community

Phylogenetic analyses have revealed that the traditional order Prasiolales, which contains filamentous and pseudoparenchymatous genera Prasiola and Rosenvingiella with complex life cycle, also contains taxa of more simple morphology such as coccoids like Pseudochlorella and Edaphochlorella or rod-like organisms like Stichococcus and Pseudostichococcus (called Prasiola clade of the Trebouxiophyceae). Recent studies have shown a high biodiversity among these organisms and questioned the traditional generic and species concept. We studied 34 strains assigned as Stichococcus, Pseudostichococcus, Diplosphaera and Desmocococcus. Phylogenetic analyses using a multigene approach revealed that these strains belong to eight independent lineages within the Prasiola clade of the Trebouxiophyceae. For testing if these lineages represent genera, we studied the secondary structures of SSU and ITS rDNA sequences to find genetic synapomorphies. The secondary structure of the V9 region of SSU is diagnostic to support the proposal for separation of eight genera. The complex taxonomic history was summarized and revised. The ITS-2/CBC approach was used for species delimitation. Considering all these results, we revised the genera Stichococcus, Pseudostichococcus, Diplosphaera and Desmococcus and proposed four new genera and four new species for the science community. The usage of the V9 region and the ITS-2 barcodes discovered potential new species among the Stichococcus-like organisms in culture-independent studies.

A new species of black fly (Diptera: Simuliidae) in the Simulium (Simulium) multistriatum species-group from Thailand

Zootaxa ◽  
2019 ◽  
Vol 4586 (3) ◽  
pp. 461 ◽  
Author(s):  
JIRAPORN THAIJARERN ◽  
KOMGRIT WONGPAKAM ◽  
ANONGRIT KANGRANG ◽  
PAIROT PRAMUAL
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Pupal Stage ◽  
Species Group ◽  
Mountainous Area ◽  
Black Fly ◽  
Interspecific Genetic Distance

A new black fly species of the Simulium multistriatum species-group of the subgenus Simulium Latreille is described from the mountainous area in northeastern Thailand, based on morphology and mitochondrial DNA sequences. The new species is morphologically similar to S. laui Takaoka and Sofian-Azirun and S. lacduongense Takaoka and Ya’cob originally described from Vietnam, S. fenestratum Edwards originally described from Indonesia and S. chaliowae Takaoka and Boonkemtong originally described from Thailand, but can be distinguished in the adult stage by the number of upper eye facets and globular shape of the spermatheca and in the pupal stage by the cocoon and shape of thoracic tubercles. Genetic distance and phylogenetic analyses of mitochondrial cytochrome c oxidase I sequences differentiated the new species from other members of S. multistriatum species-group. All specimens of the new species formed a monophyletic clade with strong support in all phylogenetic analyses. The minimum interspecific genetic distance of 4.9% is considerably greater than the new species maximum intraspecific genetic distance (2.7%). 

scholarly journals Two new entomopathogenic species of Ophiocordyceps in Thailand

MycoKeys ◽  
2019 ◽  
Vol 47 ◽  
pp. 53-74 ◽  
Author(s):  
Yuan-Pin Xiao ◽  
Sinang Hongsanan ◽  
Kevin D. Hyde ◽  
Siraprapa Brooks ◽  
Ning Xie ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Similar Species ◽  
Closely Related Species ◽  
The Family ◽  
Distinct Lineage ◽  
Morphological Differences ◽  
A New Species ◽  
Rpb1 Sequence

Ophiocordyceps is entomopathogenic and the largest studied genus in the family Ophiocordycipitaceae. Many species in this genus have been reported from Thailand. The first new species introduced in this paper, Ophiocordycepsglobiceps, differs from other species based on its smaller perithecia, shorter asci and secondary ascospores and additionally, in parasitising fly species. Phylogenetic analyses of combined LSU, SSU, ITS, TEF1α and RPB1 sequence data indicate that O.globiceps forms a distinct lineage within the genus Ophiocordyceps as a new species. The second new species, Ophiocordycepssporangifera, is distinguished from closely related species by infecting larvae of insects (Coleoptera, Elateridae) and by producing white to brown sporangia, longer secondary synnemata and shorter primary and secondary phialides. We introduce O.sporangifera based on its significant morphological differences from other similar species, even though phylogenetic distinction is not well-supported.

Description of a new species of the genus Anguillonema Fuchs, 1938 (Nematoda: Sphaerularioidea) with an identification key to the species

2018 ◽  
Vol 93 (04) ◽  
pp. 504-512
Author(s):  
A. Yaghoubi ◽  
E. Pourjam ◽  
M. Pedram
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Thin Body ◽  
Molecular Characteristics ◽  
Lateral Field ◽  
Phylogenetic Studies ◽  
Sister Clade ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
18S Rdna Sequence

AbstractAnguillonema iranicum n. sp. is described and illustrated as the second species of this genus from Iran, based on morphological, morphometric and molecular characteristics. It is identified by a short, thin body, a continuous lip region, six lines on the lateral field, a short, thin stylet, a posteriorly located pharyngo-intestinal junction to excretory pore, the presence of a post-vulval uterine sac, vulval position at 89% (87.4–89.9%) of body length, an elongate conoid tail with a rounded to pointed tip and not dorsally bent, and common functional males with short spicules and lacking a bursa. Morphological differences between the new species and the three known species of the genus, namely A. amolensis, A. crenati and A. poligraphi, are discussed. Molecular phylogenetic studies of the new species using partial 18S rDNA sequence revealed that it formed a sister clade with three species of Howardula, one species of Anguillonema and one unidentified isolate. In phylogenetic analyses using partial sequences of 28S rDNA D2-D3 segment, the new species formed a clade with two isolates of Parasitylenchus. A key to identification of Anguillonema species is also presented.

Cadophora meredithiae and C. interclivum, new species from roots of sedge and spruce in a western Canada subalpine forest

Mycologia ◽  
2018 ◽  
Vol 110 (1) ◽  
pp. 201-214 ◽  
Author(s):  
Walsh ◽  
Duan ◽  
Mehdi ◽  
Naphri ◽  
Khiste ◽  
...  
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Subalpine Forest ◽  
Western Canada ◽  
Ecological Studies ◽  
High Similarity ◽  
Study Results ◽  
Evolutionary Studies

Two new species of Cadophora are described based on multigene phylogenetic analyses and phenotypic and ecological characters. The species delimitation was based on concordance of gene genealogies. The cultures of the Cadophora species were isolated from the roots of long-beaked sedge and white spruce from a subalpine forest in western Canada; however, they probably have a broader distribution because their internal transcribed spacer (ITS) sequences have high similarity with a number of GenBank sequences from ecological studies of plant roots. The taxonomy of Cadophora in Leotiomycetes is discussed based on the phylogeny generated in this study. Results from this work will facilitate ecological and evolutionary studies on root-associated fungi.

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