scholarly journals New or little-known Boreoheptagyia (Diptera, Chironomidae) in China inferred from morphology and DNA barcodes

ZooKeys ◽  
2021 ◽  
Vol 1040 ◽  
pp. 187-200
Author(s):  
Xiao-Long Lin ◽  
Hai-Jun Yu ◽  
Xin-Hua Wang ◽  
Wen-Jun Bu ◽  
Chun-Cai Yan ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Male Adult ◽  
Male And Female ◽  
Associated Data

The male adult of Boreoheptagyia zhengi Lin & Liu, sp. nov. is described and illustrated based on material collected in China. Associated morphological characteristics and reference to its DNA barcode are provided. Boreoheptagyia kurobebrevis (Sasa & Okazawa, 1992) is newly recorded from China based on both a male and female, with additional associated data on the DNA barcode of the male adult. A neighbor-joining tree based on available Boreoheptagyia DNA barcodes and a key to the adults of Boreoheptagyia from China are given.

scholarly journals A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae)

Genes ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 637 ◽  
Author(s):  
Mengyue Guo ◽  
Yanqin Xu ◽  
Li Ren ◽  
Shunzhi He ◽  
and Xiaohui Pang
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Identification Accuracy ◽  
Dna Barcodes ◽  
Success Rates ◽  
Data Set ◽  
Herba Epimedii ◽  
Potential Risks ◽  
Medicinal Properties ◽  
Species Being

Genus Epimedium consists of approximately 50 species in China, and more than half of them possess medicinal properties. The high similarity of species’ morphological characteristics complicates the identification accuracy, leading to potential risks in herbal efficacy and medical safety. In this study, we tested the applicability of four single loci, namely, rbcL, psbA-trnH, internal transcribed spacer (ITS), and ITS2, and their combinations as DNA barcodes to identify 37 Epimedium species on the basis of the analyses, including the success rates of PCR amplifications and sequencing, specific genetic divergence, distance-based method, and character-based method. Among them, character-based method showed the best applicability for identifying Epimedium species. As for the DNA barcodes, psbA-trnH showed the best performance among the four single loci with nine species being correctly differentiated. Moreover, psbA-trnH + ITS and psbA-trnH + ITS + rbcL exhibited the highest identification ability among all the multilocus combinations, and 17 species, of which 12 are medicinally used, could be efficiently discriminated. The DNA barcode data set developed in our study contributes valuable information to Chinese resources of Epimedium. It provides a new means for discrimination of the species within this medicinally important genus, thus guaranteeing correct and safe usage of Herba Epimedii.

scholarly journals DNA barcodes and morphology of P. krempfi, P. mekongensis, and P. elongatus

2016 ◽  
Vol 14 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Dương Thúy Yên ◽  
Nguyễn Kiệt ◽  
Bùi Sơn Nên ◽  
Nguyễn Văn Thường ◽  
Nguyễn Bạch Loan ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Mekong Delta ◽  
Genetic Distances ◽  
Dna Barcodes ◽  
Gill Rakers ◽  
Coi Sequences ◽  
Relationship Of ◽  
Metric Traits ◽  
I Gene

Three Pangasius species including P. krempfi, P. elongatus and P. mekongensis, are economically important. They can be mis-identified due to similar external appreance at small sizes. This study aimed to distinguish these species based on their differences in DNA barcode, COI (cytochrome c oxidase subunit I) gene, and morphological characteristics. Fish with various sizes (>90 samples/species) were sampled at the lower Mekong delta region. Kimura-2 parameter genetic distances based on COI sequences of three species (15 samples, in which, 4 unique sequences were assigned Genbank accession numbers from KT289877 to KT289880) are relatively high, ranging 9.33 – 12.10 %. Morphological measurements show that coutanble traits including numbers of fin rays and the first gill rakers vary in similar ranges but ratios of metric traits are significantly different among three species (P<0.01). Principle component analysis using metric traits sets three species apart. P. elongatus is characterized by elongated body, long caudal preduncle, large eyes, and retangle palatine tooth plates. P. krempfi differs from P. mekongesis in characteristics on their head. The number of sections, shape and length of barbel are different among three species. Phylogenetic relationship of three species based on morphology and COI sequences indicate that P. krempfi is closer to P. mekongenis rather than P. elongatus, and that the distance between P. mekongenis and P. elongatus is the largest.

A new species of Manoa Fittkau (Diptera: Chironomidae), with DNA barcodes from Xianju National Park, Oriental China

Zootaxa ◽  
2017 ◽  
Vol 4231 (3) ◽  
pp. 398 ◽  
Author(s):  
XIN QI ◽  
XIN-HUA WANG ◽  
TROND ANDERSEN ◽  
XIAO-LONG LIN
Keyword(s):  
New Species ◽  
Adult Male ◽  
National Park ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Male And Female ◽  
Oriental Region ◽  
First Time ◽  
A New Species

The genus Manoa and the tribe Pseudochironomini are recorded from the Oriental region for the first time. Manoa xianjuensis Qi & Lin sp. n. from Xianju National Park, Zhejiang, China is described and illustrated as adult male and female, the latter associated with the male by standard DNA barcodes. A neighbor joining tree based on available Pseudochironomini DNA barcodes and keys to the adults in Manoa are given. 

scholarly journals Three cryptic Anaplecta (Blattodea, Blattoidea, Anaplectidae) species revealed by female genitalia, plus seven new species from China

ZooKeys ◽  
2022 ◽  
Vol 1080 ◽  
pp. 53-97
Author(s):  
Jing Zhu ◽  
Jiawei Zhang ◽  
Xinxing Luo ◽  
Zongqing Wang ◽  
Yanli Che
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Morphological Characteristics ◽  
The Other ◽  
Female Genitalia ◽  
Dna Barcodes ◽  
Male And Female

Morphological characteristics, including male and female genitalia, combined with DNA barcodes were used to identify 470 Anaplecta specimens sampled from China. Ten Anaplecta species are new to science, including three cryptic species: A. paraomei Zhu &amp; Che, sp. nov., A. condensa Zhu &amp; Che, sp. nov., and A. longihamata Zhu &amp; Che, sp. nov., which are distinguished mainly by their female genitalia. The other seven new species are as follows: A. bicruris Zhu &amp; Che, sp. nov., A. spinosa Zhu &amp; Che, sp. nov., A. ungulata Zhu &amp; Che, sp. nov., A. anomala Zhu &amp; Che, sp. nov., A. serrata Zhu &amp; Che, sp. nov., A. bombycina Zhu &amp; Che, sp. nov., and A. truncatula Zhu &amp; Che, sp. nov. This study illustrates that differences in female genitalia can be used to distinguish among species of Anaplecta. The female genitalia of 19 Chinese Anaplecta species are described and illustrated in this paper.

scholarly journals Efficiency of ITS1-5.8S-ITS2 region in identifying Cordyceps species

2020 ◽  
Vol 16 (4) ◽  
pp. 705-712
Author(s):  
Le Thi Thu Hien ◽  
Ha Hong Hanh
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Nearest Neighbor ◽  
Dna Barcode ◽  
Morphological Characteristics ◽  
Pcr Amplification ◽  
Cordyceps Sinensis ◽  
Its2 Region ◽  
Dna Barcodes ◽  
Plastid Genomes

Cordyceps genus is a well-known traditional medicine worldwide. It contains abundant physiological active compounds that were demonstrated to perform benefit in reducing progression of cancer as well as protecting human health. Accurately classifying species in this genus is essential in order to prevent commercial counterfeit medicines. Nowadays, a taxonomic classification of species based on DNA sequences can overcome the existed limitation in identifying by using only morphological characteristics of this genus. DNA barcodes are standard short genomic regions that are universally present in target lineages and has sufficient sequence variation to discriminate species in the genus. A variety of loci has been suggested as DNA barcodes for plants, including genes and non-coding regions in the nuclear and plastid genomes such as psbA-trnH, matK, rbcL, and ITS. Thus, the objective of this study was to identify selected species of Cordyceps genus using DNA barcodes. Seven strains of Cordyceps were collected. Total DNA extraction and purification, PCR amplification and DNA sequencing were performed with standard chemicals and kits. The candidate ITS1-5.8S-ITS2 region was amplified and sequenced. Data were analyzed using Bioedit 7.2.6 and MEGA 7 softwares. Analysis of seven obtained DNA barcode sequences of collected samples revealed that the ITS1-5.8S-ITS2 region provided high species discriminating power for Cordyceps genus. Accordingly, phylogenetic trees based on this DNA barcode exhibited six samples had closed relationship to Cordyceps militaris, while another specimen was the nearest neighbor to Cordyceps sinensis with average similarities at 99.82% and 99.81%, respectively. Our results support the identification of valuable medicinal plant species within Cordyceps genus.

scholarly journals The leaf-mining genus Antispila Hübner, 1825 feeding on Vitaceae in Shandong Peninsula, China with one new species (Lepidoptera, Heliozelidae)

ZooKeys ◽  
2018 ◽  
Vol 744 ◽  
pp. 49-65 ◽  
Author(s):  
Nan Wang ◽  
Tengteng Liu ◽  
Jiasheng Xu ◽  
Bin Jiang
Keyword(s):  
Host Plant ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
New Host ◽  
Shandong Peninsula ◽  
Male And Female ◽  
Leaf Mining ◽  
Female Host ◽  
First Time ◽  
One New Species

TheAntispilaspecies feeding on Vitaceae from Shandong Peninsula, China are treated.AntispilakunyuensisLiu,sp. n., feeding onAmpelopsishumulifolia, is described as new to science, andA.uenoiKuroko, 1987, feeding onVitisamurensis, is recorded as new for China.Vitisamurensisis documented as a new host plant forA.uenoi. The adult male and female, host plant and typical patterns of leaf-mines of both species are illustrated, as are male and female genitalia and venation. The venation and the paired tufts of scales on the 7thabdominal segment in male are illustrated forA.uenoifor the first time. DNA barcodes of both species are also provided, together with a neighbor-joining tree for facilitating species delimitation.

Applicability of chloroplast DNA barcodes for wood identification between Santalum album and its adulterants

Holzforschung ◽  
2019 ◽  
Vol 73 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Lichao Jiao ◽  
Tuo He ◽  
Eleanor E. Dormontt ◽  
Yonggang Zhang ◽  
Andrew J. Lowe ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Dna Barcode ◽  
Species Discrimination ◽  
Santalum Album ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Discrimination Ability ◽  
East Indian ◽  
Commercial Market ◽  
Increasing Demand

AbstractThe East Indian sandalwood,Santalum albumL., is known for its fragrant heartwood and extractive oils. The increasing demand for the valuable sandalwood products has led to illegal and excessive logging, and there are otherSantalumspecies in the commercial market as substitutes (adulterants). Improved tools for the identification ofSantalumspecies are needed to control this situation. In this study, four chloroplast DNA (cpDNA) regions were screened (matK,psbA-trnH,trnK andtrnL) on 35 vouchered specimens covering fiveSantalumspecies, i.e.Santalum acuminatum,S. album,Santalum lanceolatum,Santalum murrayanumandSantalum spicatum. The goal of this study was to test the species discrimination ability by means of both the TaxonDNA and neighbor-joining (NJ) methods. The results indicate that the combination ofpsbA-trnH+trnK gave the best performance for discrimination (100%) of the studiedSantalumspecies concerning the discrimination ability and recovery rate. Meanwhile, six unvouchered wood specimens were retrieved and accurately identified at the species level based on the recommended DNA barcodes. The DNA barcode method is now ready for application in the monitoring of the trade of this valuable resource, and provides an effective approach for wood species identification and product authentication.

A case of gynandromorphism in Amblyomma mixtum (Acari, Ixodidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 268
Author(s):  
Fredy A. Rivera-Páez ◽  
Marcelo B. Labruna ◽  
Thiago F. Martins ◽  
Bruno Rodrigues-Sampieri ◽  
María I. Camargo-Mathias
Keyword(s):  
Tick Species ◽  
Morphological Characteristics ◽  
Amblyomma Cajennense ◽  
Literature Report ◽  
Male And Female ◽  
Natural Infestation

Gynandromorphism is a condition in which an organism simultaneously exhibits male and female morphological characteristics. In Colombia, the taxon Amblyomma cajennense is represented by the species Amblyomma patinoi and Amblyomma mixtum. In September of 2014, in the Colombian Orinoco region, adult ticks were collected and determined from natural infections in bovines and equines. A gynandromorph was described from a natural infestation on a bovine, and morphologically classified as A. mixtum. This is the first literature report of a gynandromorph of A. mixtum, and the first description of a gynandromorph for a tick species in Colombia.

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