scholarly journals Utilization of Component-Resolved Diagnosis in Precision Allergology

2020 ◽  
Vol 95 (1) ◽  
pp. 13-17
Author(s):  
Jae-Hyun Lee
Keyword(s):  
House Dust ◽  
Grass Pollen ◽  
Allergic Diseases ◽  
Major Allergen ◽  
House Dust Mites ◽  
Dust Mites ◽  
Allergen Specific Immunotherapy ◽  
Component Resolved Diagnosis ◽  
Group I ◽  
Amb A 1

It is very important to identify the causal allergen of each patient in the diagnosis and treatment of allergic diseases. An experimental method of specific IgE testing for single clinically meaningful antigens is called component resolved diagnosis (CRD). Since the introduction of CRD, many single antigens with clinical significance from animal and plant allergenic source have become known. Bet v 1, the major allergen of white birch pollen, can be an indication marker for allergen-specific immunotherapy if the patient is sensitized. Phl p 1, the group I allergen of timothy grass pollen, is an important marker that suggests that patients are truly sensitized to grass pollen. The major allergen of ragweed pollen, Amb a 1 and Art v 1, which is the major allergen of mugwort pollen, are clinically important to distinguish true sensitization. House dust mites have several clinically useful component allergens. Der p 1 and Der p 2 are the initiation molecules in the molecular spreading of allergic diseases caused by house dust mites. Among food allergens, Ara h 2 from peanut, which is closely related to anaphylaxis among several clinical manifestation of peanut allergy. Tri a 19, well known to omega-5 gliadin is important in patients with wheat-dependent exercise-induced anaphylaxis. It is conceivable that the molecular biological concept of CRD methods should lead to ‘patient-customized treatment’ beyond ‘diagnosis’ in the future. Primary prevention of the allergic diseases will apply, which will conceptually be called ‘allergen immunprophylaxis’. This is the right direction of future precision allergology.

scholarly journals HDM exposure enhances subsequent responses to OVA-induced food allergy

2021 ◽  
Author(s):  
Jianli Lin ◽  
Desheng Chen ◽  
Kexin Chang ◽  
Dan Li ◽  
Lvxin Guan ◽  
...  
Keyword(s):  
Food Allergy ◽  
House Dust ◽  
Intestinal Barrier ◽  
Allergic Diseases ◽  
Serum Levels ◽  
House Dust Mites ◽  
Lower Body ◽  
Dust Mites ◽  
Barrier Dysfunction ◽  
Control Groups

Abstract HDM (House dust mites) are important environmental trigger factors of airway allergic diseases, the allergens of HDM were detected in the human gut mucosa, which induces local inflammation and increases intestinal permeability. This study tests a hypothesis that house dust mites contribute to the development of OVA (ovalbumin)-induced food allergy. The serum levels of IgE against HDM in patients with food allergy were detected with UniCAP100 (Pharmacia, Uppsala, Sweden); the HDM-induced/the OVA-induced mouse model of food allergy was developed. Difference between 2 groups was determined by Student t test or ANOVA if more than two groups. Compared to the healthy controls, the patients with food allergy have higher levels of serum IgE against HDM. Compared to food allergy alone groups, the levels of IgE against HDM in food allergy with asthma or allergic rhinitis groups were increased significantly. In mouse models, we found that HDM/OVA induced allergy-like symptoms, lower body temperature, and lower body weight. The levels of IgE, IgG1, mMCP-1 (mouse mast cell protease-1), IL-4 and IL-5 in the HDM and HDM + CT (cholera toxin) groups were higher than the control groups, and the levels of IgE, IgG1, IL-4 and IL-5 in the HDM, OVA and HDM + OVA groups were higher than the control groups. The pathology of intestinal tract in the HDM and HDM + CT/the HDM, OVA and HDM + OVA groups were more severe and exhibited more eosinophils than the control groups. Moreover, the prior exposure to HDM induced intestinal barrier dysfunction, and facilitated the development of intestinal allergy in mice. Based on above data and previous researches, we put forward that HDM exposure enhances subsequent responses to OVA-induced food allergy.

HOUSE DUST MITE ALLERGENS: FROM NATIVE EXTRACTS TO MODIFIED PREPARATIONS

2021 ◽  
Author(s):  
Anna Vasilyeva ◽  
Valentina M. Berzhets ◽  
Nina S. Petrova ◽  
Svetlana V. Khlgatian ◽  
Stanislava Yu. Petrova ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Binding Reaction ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Elisa Inhibition

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

scholarly journals 96 Sublingual allergen-specific immunotherapy in pediatric patients: biomarkers and molecular allergy diagnostics as predictors of therapeutic success

2020 ◽  
Vol 25 (Supplement_2) ◽  
pp. e40-e40
Author(s):  
Lala Allahverdiyeva ◽  
G E Agaragimova ◽  
Veronica Mugarab Samedi
Keyword(s):  
House Dust ◽  
Pediatric Patients ◽  
Specific Immunotherapy ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Healthy Children ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Molecular Allergy

Abstract Background Efficacy of allergen-specific immunotherapy (ASIT) in patients with asthma could be significantly enhanced when physicians have an opportunity to identify those who are most likely to respond, determine when to stop treatment, and predict relapse. Thus, molecular allergy diagnostic is an effective tool that could help to individualize the diagnosis and treatment of asthma by accurate detection of the causative and cross-reactive allergens in pediatric patients. Cytokines are known as reliable diagnostic and prognostic biomarkers for asthma and can be used to evaluate the therapeutic effect. Objectives To evaluate the roles of molecular allergy diagnostic and biomarkers (L-10, IFNγ и TGFβ) as predictors of ASIT efficacy in pediatric patients with asthma. Design/Methods Eighty children aged 5 to 18 years with mild and moderate atopic bronchial asthma were included in the study. Sixty-eight out of 80 (85%) were found to have sensitization to house dust mites Dermatophagoides Pteronyssinus and/or Dermatophagoides Farinae. All these patients underwent molecular diagnostics using ImmunoCAP (Phadia 100) to determine the major (r Der p1, r Der p 2) and minor allergens (r Der p10) of the house dust mites. Twenty-eight patients with mild and moderate asthma were found to have a high sensitivity to these allergens and received SLIT with a mixture of house dust mites (Dermatophagoides Pteronyssinus and/or Dermatophagoides Farinae). To compare the efficacy of SLIT, we evaluated the severity of asthma clinical symptoms on a point scale from 0 to 3, and biomarker levels (IL-10, IFNγ и TGFβ) before and 6 months after SLIT. Twelve healthy children were included in the study as a reference group. Results At the beginning of the study, the average daily score in the SLIT group of 2.5 ± 0.7, and after 6 months of treatment, it decreased to 0.5 ± 0.2. The levels of biomarkers were markedly decreased prior to the treatment, especially TGFβ levels, and normalized at the end of treatment (Tab.1). Conclusion These findings support the efficacy of molecular diagnosis to determine the efficacy of allergen-specific immunotherapy in patients with asthma.

Month Of Birth And Sensitization To Grass Pollen Or House Dust Mites: Is There Association?

2009 ◽  
Vol 123 (2) ◽  
pp. S22-S22
Author(s):  
A.C. Porto Neto ◽  
J.R. de Dias ◽  
C.M.Z. Rech ◽  
J. Pastorello ◽  
P.F. Magrin ◽  
...  
Keyword(s):  
House Dust ◽  
Grass Pollen ◽  
House Dust Mites ◽  
Dust Mites ◽  
Month Of Birth

scholarly journals Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
N. Khantavee ◽  
C. Chanthick ◽  
A. Tungtrongchitr ◽  
N. Techakriengkrai ◽  
S. Suradhat ◽  
...  
Keyword(s):  
House Dust ◽  
Characteristic Curve ◽  
False Positive Rate ◽  
Specific Ige ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Intradermal Testing ◽  
Positive Rate

Abstract Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy (n = 33) and atopic dogs (AD) (n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p = 1.00). High levels of all antibody isotypes (IgE, IgG, IgG1 and IgG2) against HDM were found in aged healthy dogs (> 3 years old). In AD, HDM-IgE and IgG1 levels were higher in dogs that were IDT positive to HDM than in IDT negative animals. Levels of IgE and IgG1 could be used to distinguish the specific allergens, whereas total IgG and IgG2 levels were not different between IDT-positive and IDT-negative AD. By the receiver operating characteristic curve at a false-positive rate = 0.10, both IgE and IgG1 showed better sensitivity than IgG and IgG2. Similar to IgE, serum IgG1 concentration was also relevant to IDT outcomes. Conclusions Our in-house ELISAs coated with local HDM were useful for evaluating antibody levels, and we propose use of the HDM-specific IgG1 subclass as a biomarker to detect HDM specific allergens in AD, potentially together with an IgE based platform.

scholarly journals TUNGAU DEBU RUMAH YANG DITEMUKAN DI KELURAHAN PERKAMIL KECAMATAN PAAL 2 KOTA MANADO

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Regina F. R. De Breving
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Living Room ◽  
Dust Mites ◽  
Dust Mite ◽  
Descriptive Survey

Abstract: In house dust are a lot of house dust mites that found in damp houses, cotton mattress, pillows, bolsters, and other home furnishings. Sources of dust containing house dust mites are most in the bedroom  especially cotton mattress. Data from distric health centers Paal 2 found that there are many people who suffer from asthma and allergic diseases, coupled with the humid air in Manado City to allow the development of house dust mites in the area. The purpose of this research is to determine the species and density of house dust mites in urban Perkamil Paal 2 districts Manado city. This research method is descriptive survey to obtain data on the species and density of house dust mites by simple random sampling method. The results found 5 species of house dust mites are Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp and Cheyletus spp in bedrooms and living room. House dust mite densities obtained an average of 2,33 in bedroom and 2,07 in living room. The conclusion is house dust mite Dermatophagoides spp most commonly found in bedrooms and living room. Keywords: species, density, house dust mite   Abstrak: Dalam debu rumah terdapat banyak tungau debu rumah yang ditemukan pada rumah yang lembab, kasur kapuk, bantal, guling serta perabot rumah yang lain. Sumber debu yang mengandung tungau debu rumah terbanyak adalah debu kamar tidur terutama debu di kasur kapuk. Dari data Puskesmas Kecamatan Paal 2 didapatkan bahwa masih banyak masyarakat yang menderita asma dan penyakit alergi, ditambah lagi dengan udara Kota Manado yang lembab sehingga memungkinkan terjadinya perkembangan tungau debu rumah di daerah tersebut. Tujuan penelitian ini untuk mengetahui jenis dan kepadatan tungau debu rumah di Kelurahan Perkamil Kecamatan Paal 2 Kota Manado. Metode penelitian ini secara survei deskriptif untuk mendapatkan data tentang jenis dan kepadatan tungau debu rumah dengan metode simple random sampling. Hasil yang didapat ditemukan 5 jenis tungau debu rumah yaitu Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp dan Cheyletus spp di ruang tidur dan ruang tamu. Kepadatan tungau debu rumah didapatkan rata-rata 2,33 di ruang tidur dan 2,07 di ruang tamu. Kesimpulan yang diambil yaitu tungau debu rumah Dermatophagoides spp paling banyak ditemukan di ruang tidur maupun ruang tamu. Kata kunci: jenis, kepadatan, tungau debu rumah

Sign in / Sign up

Export Citation Format

Share Document