The protective role of melatonin under heavy metal-induced stress in Melissa Officinalis L.

Abstract The aim of this study was to evaluate the potential protective role of Agrimonia eupatoria L. in heavy metal induced nephrotoxicity. Rabbit kidney epithelial cells (RK13) were used as the model cell line. They were exposed to three different heavy metal compounds: cadmium chloride dihydrate CdCl2.2H2O (15 and 20 mg.l−1), potassium dichromate K2Cr2O7 (1, 10 mg.l−1), and zinc sulfate heptahydrate ZnSO4.7H2O (50, 150 mg.l−1) simultaneously with agrimony (ethanolic extract, 100 mg.l−1). The cell response was recorded using the xCELLigence system or real-time cell analysis (RTCA) as a cell index (CI) and expressed as cell adherence (%) compared to control cells without treatment. The potential nephroprotective effects were recorded in cells treated with chromium (1 a 10 mg.l−1) and agrimony, where the cell adherence increased from 95.11 ± 11.25 % and 7.24 ± 0.33 % to 103.26 ± 1.23 % and 68.54 ± 4.89 % (P < 0.05) respectfully and also with a combination of agrimony and zinc (150 mg.l−1), where the adherence increased from 57.45 ± 1.98 % to 95.4 ± 6.95 %. During the cell exposure to cadmium in combination with agrimony, the protective effect was not recorded; the adherence of cells was even decreased (P < 0.05).

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Buckwheat (Fagopyrum esculentumMoench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+and Cd2+Treatments

Journal of Agricultural and Food Chemistry ◽

10.1021/jf904483a ◽

2010 ◽

Vol 58 (6) ◽

pp. 3488-3494 ◽

Cited By ~ 15

Author(s):

Dragana B. Nikolić ◽

Jelena T. Samardžić ◽

Ana M. Bratić ◽

Ivan P. Radin ◽

Srdjan P. Gavrilović ◽

...

Keyword(s):

Heavy Metal ◽

Promoter Region ◽

Heavy Metal Stress ◽

Protective Role ◽

Metal Stress

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Protective role of selenium in the shortening of telomere length in newborns induced by in utero heavy metal exposure

Environmental Research ◽

10.1016/j.envres.2020.109202 ◽

2020 ◽

Vol 183 ◽

pp. 109202 ◽

Cited By ~ 1

Author(s):

Kyi Mar Wai ◽

Masahiro Umezaki ◽

Mitsutoshi Umemura ◽

Ohn Mar ◽

Chiho Watanabe

Keyword(s):

Heavy Metal ◽

Telomere Length ◽

In Utero ◽

Protective Role ◽

Metal Exposure ◽

Heavy Metal Exposure

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Article Commentary: Chelation Therapy for Mercury Poisoning

Biochemistry Insights ◽

10.4137/bci.s3036 ◽

2009 ◽

Vol 2 ◽

pp. BCI.S3036 ◽

Cited By ~ 1

Author(s):

Rong Guan ◽

Han Dai

Keyword(s):

Heavy Metal ◽

Chelating Agents ◽

Chelation Therapy ◽

Research Direction ◽

Protective Role ◽

Mercury Poisoning ◽

Heavy Metal Poisoning ◽

Metal Poisoning ◽

Major Treatment

Chelation therapy has been the major treatment for heavy metal poisoning. Various chelating agents have been developed and tested for treatment of heavy metal intoxications, including mercury poisoning. It has been clearly shown that chelating agents could rescue the toxicity caused by heavy metal intoxication, but the potential preventive role of chelating agents against heavy metal poisoning has not been explored much. Recent paper by Siddiqi and colleagues has suggested a protective role of chelating agents against mercury poisoning, which provides a promising research direction for broader application of chelation therapy in prevention and treatment of mercury poisoning.

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Protective role of Melissa officinalis L. extract on liver of hyperlipidemic rats: A morphological and biochemical study

Journal of Ethnopharmacology ◽

10.1016/j.jep.2005.02.038 ◽

2005 ◽

Vol 99 (3) ◽

pp. 391-398 ◽

Cited By ~ 53

Author(s):

S. Bolkent ◽

R. Yanardag ◽

Omur Karabulut-Bulan ◽

B. Yesilyaprak

Keyword(s):

Biochemical Study ◽

Protective Role ◽

Melissa Officinalis

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Melatonin confers heavy metal-induced tolerance by alleviating oxidative stress and reducing the heavy metal accumulation in Exophiala pisciphila, a dark septate endophyte (DSE)

BMC Microbiology ◽

10.1186/s12866-021-02098-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Yang Yu ◽

Zhaowei Teng ◽

Zongmin Mou ◽

Yan Lv ◽

Tao Li ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metal ◽

Stress Tolerance ◽

Metal Accumulation ◽

Heavy Metal Accumulation ◽

Biosynthetic Enzyme ◽

Dark Septate Endophyte ◽

Induced Stress ◽

Exophiala Pisciphila

Abstract Background Melatonin (MT), ubiquitous in almost all organisms, functions as a free radical scavenger. Despite several reports on its role as an antioxidant in animals, plants, and some microorganisms, extensive studies in filamentous fungi are limited. Based upon the role of melatonin as an antioxidant, we investigated its role in heavy metal-induced stress tolerance in Exophiala pisciphila, a dark septate endophyte (DSE), by studying the underlying mechanisms in alleviating oxidative stress and reducing heavy metal accumulation. Results A significant decrease in malondialdehyde (MDA) and oxygen free radical (OFR) in E. pisciphila was recorded under Cd, Zn, and Pb stresses as compared to the control. Pretreatment of E. pisciphila with 200.0 μM exogenous melatonin significantly increased the activity of superoxide dismutase (SOD) under Zn and Pb stresses. Pretreatment with 200.0 μM melatonin also lowered Cd, Zn, and Pb concentrations significantly. Melatonin production was enhanced by Cd, Cu, and Zn after 2 d, and melatonin biosynthetic enzyme genes, E. pisciphila tryptophan decarboxylase (EpTDC1) and serotonin N-acetyltransferase (EpSNAT1), were transcriptionally upregulated. The overexpression of EpTDC1 and N-acetylserotonin O-methyltransferase (EpASMT1) in Escherichia coli and Arabidopsis thaliana enhanced its heavy metal-induced stress tolerance. The overexpression of EpTDC1 and EpASMT1 reduced the Cd accumulation in the whole A. thaliana plants, especially in the roots. Conclusions Melatonin conferred heavy metal-induced stress tolerance by alleviating oxidative stress, activating antioxidant enzyme SOD, and reducing heavy metal accumulation in E. pisciphila. Melatonin biosynthetic enzyme genes of E. pisciphila also played key roles in limiting excessive heavy metal accumulation in A. thaliana. These findings can be extended to understand the role of melatonin in other DSEs associated with economically important plants and help develop new strategies in sustainable agriculture practice where plants can grow in soils contaminated with heavy metals.

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Exopolysaccharide II Is Relevant for the Survival of Sinorhizobium meliloti under Water Deficiency and Salinity Stress

Molecules ◽

10.3390/molecules25214876 ◽

2020 ◽

Vol 25 (21) ◽

pp. 4876

Author(s):

Emiliano Primo ◽

Pablo Bogino ◽

Sacha Cossovich ◽

Emiliano Foresto ◽

Fiorela Nievas ◽

...

Keyword(s):

Sinorhizobium Meliloti ◽

Protective Role ◽

Environmental Stressors ◽

Saline Stress ◽

Symbiotic Association ◽

Atmospheric Nitrogen ◽

Water Deficiency ◽

Wild Type ◽

Induced Stress

Sinorhizobium meliloti is a soil bacterium of great agricultural importance because of its ability to fix atmospheric nitrogen in symbiotic association with alfalfa (Medicago sativa) roots. We looked into the involvement of exopolysaccharides (EPS) in its survival when exposed to different environmental stressors, as well as in bacteria–bacteria and bacteria–substrate interactions. The strains used were wild-type Rm8530 and two strains that are defective in the biosynthesis of EPS II: wild-type Rm1021, which has a non-functional expR locus, and mutant Rm8530 expA. Under stress by water deficiency, Rm8530 remained viable and increased in number, whereas Rm1021 and Rm8530 expA did not. These differences could be due to Rm8530′s ability to produce EPS II. Survival experiments under saline stress showed that viability was reduced for Rm1021 but not for Rm8530 or Rm8530 expA, which suggests the existence of some regulating mechanism dependent on a functional expR that is absent in Rm1021. The results of salinity-induced stress assays regarding biofilm-forming capacity (BFC) and autoaggregation indicated the protective role of EPS II. As a whole, our observations demonstrate that EPS play major roles in rhizobacterial survival.

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Melatonin confers heavy metal-induced tolerance by alleviating oxidative stress and reducing the heavy metal accumulation in Exophiala pisciphila, a dark septate endophyte (DSE)

10.21203/rs.3.rs-54820/v2 ◽

2020 ◽

Author(s):

Yang Yu ◽

Zhaowei Teng ◽

Zongmin Mou ◽

Yan Lv ◽

Tao Li ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metal ◽

Stress Tolerance ◽

Metal Accumulation ◽

Heavy Metal Accumulation ◽

Biosynthetic Enzyme ◽

Dark Septate Endophyte ◽

Induced Stress ◽

Exophiala Pisciphila

Abstract Background: Melatonin (MT), ubiquitous in almost all organisms, functions as a free radical scavenger. Despite several reports on its role as an antioxidant in animals, plants, and some microorganisms, extensive studies in filamentous fungi are limited. Based upon the role of melatonin as an antioxidant, we investigated its role in heavy metal-induced stress tolerance in Exophiala pisciphila, a dark septate endophyte (DSE), by studying the underlying mechanisms in alleviating oxidative stress and reducing heavy metal accumulation.Results: A significant decrease in malondialdehyde (MDA) and oxygen free radical (OFR) in E. pisciphila was recorded under Cd, Zn, and Pb stresses as compared to the control. Pretreatment of E. pisciphila with 200.0 μM exogenous melatonin significantly increased the activity of superoxide dismutase (SOD) under Zn and Pb stresses. Pretreatment with 200.0 μM melatonin also lowered Cd, Zn, and Pb concentrations significantly. Melatonin production was enhanced by Cd, Cu, and Zn after 2 d, and melatonin biosynthetic enzyme genes, E. pisciphila tryptophan decarboxylase (EpTDC1) and serotonin N-acetyltransferase (EpSNAT1), were transcriptionally upregulated. The overexpression of EpTDC1 and N-acetylserotonin O-methyltransferase (EpASMT1) in Escherichia coli and Arabidopsis thaliana enhanced its heavy metal-induced stress tolerance. The overexpression of EpTDC1 and EpASMT1 reduced the Cd accumulation in the whole A. thaliana plants, especially in the roots.Conclusions: Melatonin conferred heavy metal-induced stress tolerance by alleviating oxidative stress, activating antioxidant enzyme SOD, and reducing heavy metal accumulation in E. pisciphila. Melatonin biosynthetic enzyme genes of E. pisciphila also played key roles in limiting excessive heavy metal accumulation in A. thaliana. These findings can be extended to understand the role of melatonin in other DSEs associated with economically important plants and help develop new strategies in sustainable agriculture practice where plants can grow in soils contaminated with heavy metals.

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