scholarly journals Histopathological changes associated with white Striping and wooden breast myopathies in pectoralis major muscle and meat quality characteristics in broiler chickens subjected to feed restriction

2021 ◽  
Keyword(s):  
Meat Quality ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Quality Characteristics ◽  
Pectoralis Major ◽  
Feed Restriction ◽  
Histopathological Changes ◽  
White Striping ◽  
Wooden Breast

scholarly journals Effect of genotype, gender and feed restriction on growth, meat quality and the occurrence of white striping and wooden breast in broiler chickens

2015 ◽  
Vol 94 (12) ◽  
pp. 2996-3004 ◽  
Author(s):  
A. Trocino ◽  
A. Piccirillo ◽  
M. Birolo ◽  
G. Radaelli ◽  
D. Bertotto ◽  
...  
Keyword(s):  
Meat Quality ◽  
Broiler Chickens ◽  
Feed Restriction ◽  
White Striping ◽  
Wooden Breast

scholarly journals Ectopic Lipid Accumulation is Key Feature in Early Stages of Wooden Breast

2019 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Expression Analysis ◽  
Lipid Accumulation ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from a subset of 101 birds randomly selected from a total of 302 birds at 14 days of age, after which all birds were raised to 7 weeks of age for scoring of wooden breast. RNA sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

scholarly journals Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 746 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Lipid Metabolism ◽  
Expression Analysis ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from 101 birds at 14 days of age. Birds were subsequently raised to 7 weeks of age to allow sample selection based on the wooden breast phenotype at market age. RNA-sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p-value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

scholarly journals Increased Expression of Lipid Metabolism Genes in Early Stages of Wooden Breast Links Myopathy of Broilers to Metabolic Syndrome in Humans

2019 ◽  
Author(s):  
Juniper A. Lake ◽  
Michael B. Papah ◽  
Behnam Abasht
Keyword(s):  
Lipid Metabolism ◽  
Expression Analysis ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Differential Expression Analysis ◽  
Breast Disease ◽  
Pectoralis Major ◽  
P Value ◽  
Altered Expression ◽  
Wooden Breast

Wooden breast is a muscle disorder affecting modern commercial broiler chickens that causes a palpably firm pectoralis major muscle and severe reduction in meat quality. Most studies have focused on advanced stages of wooden breast apparent at market age, resulting in limited insights into the etiology and early pathogenesis of the myopathy. Therefore, the objective of this study was to identify early molecular signals in the wooden breast transcriptional cascade by performing gene expression analysis on the pectoralis major muscle of two-week-old birds that may later exhibit the wooden breast phenotype by market age at 7 weeks. Biopsy samples of the left pectoralis major muscle were collected from 101 birds at 14 days of age. Birds were subsequently raised to 7 weeks of age to allow sample selection based on the wooden breast phenotype at market age. RNA sequencing was performed on 5 unaffected and 8 affected female chicken samples, selected based on wooden breast scores (0 to 4) assigned at necropsy where affected birds had scores of 2 or 3 (mildly or moderately affected) while unaffected birds had scores of 0 (no apparent gross lesions). Differential expression analysis identified 60 genes found to be significant at an FDR-adjusted p value of 0.05. Of these, 26 were previously demonstrated to exhibit altered expression or genetic polymorphisms related to glucose tolerance or diabetes mellitus in mammals. Additionally, 9 genes have functions directly related to lipid metabolism and 11 genes are associated with adiposity traits such as intramuscular fat and body mass index. This study suggests that wooden breast disease is first and foremost a metabolic disorder characterized primarily by ectopic lipid accumulation in the pectoralis major.

scholarly journals Wooden Breast Myodegeneration of Pectoralis Major Muscle Over the Growth Period in Broilers

2016 ◽  
Vol 54 (1) ◽  
pp. 119-128 ◽  
Author(s):  
H.-K. Sihvo ◽  
J. Lindén ◽  
N. Airas ◽  
K. Immonen ◽  
J. Valaja ◽  
...  
Keyword(s):  
Pectoralis Major Muscle ◽  
Growth Period ◽  
Pectoralis Major ◽  
Histological Evaluation ◽  
Unknown Etiology ◽  
Focal Lesion ◽  
Dietary Selenium ◽  
Perivascular Cell ◽  
White Striping ◽  
Wooden Breast

Wooden breast (WB) myopathy of broiler chickens is a myodegenerative disease of an unknown etiology and is macroscopically characterized by a hardened consistency of the pectoralis major muscle. Our aim was to describe the development and morphology of WB over the growth period in broilers. Additionally, the effect of restricted dietary selenium on the occurrence of WB was examined by allocating the birds in 2 dietary groups: restricted and conventional level of selenium. The experiment included 240 male broilers that were euthanized at ages of 10, 18, 24, 35, 38, or 42 days and evaluated for WB based on abnormal hardness of the pectoralis major muscle. The severity and the distribution of the lesion and presence of white striping were recorded. The first WB cases were seen at 18 days; 13/47 birds (28%) were affected and the majority exhibited a mild focal lesion. In subsequent age groups the WB prevalence varied between 48% and 73% and the lesion was usually diffuse and markedly firm. White striping often coexisted with WB. Histological evaluation performed on 111 cases revealed a significant association of myodegeneration and lymphocytic vasculitis with WB. Vasculitis and perivascular cell infiltration were restricted to the veins. Restricted dietary selenium did not affect the occurrence of WB ( P = .44). Our results indicate that WB starts focally and spreads to form a diffuse and more severe lesion.

scholarly journals Quality of breast meat from broiler chickens raised in Brazil affected by white striping myopathy

2021 ◽  
Vol 10 (2) ◽  
pp. e42210212637
Author(s):  
Juliana Lolli Malagoli de Mello ◽  
Rodrigo Alves de Souza ◽  
Fábio Borba Ferrari ◽  
Erika Nayara Freire Cavalcanti ◽  
Rodrigo Fortunato de Oliveira ◽  
...  
Keyword(s):  
Meat Quality ◽  
Broiler Chickens ◽  
Pectoralis Major Muscle ◽  
Chicken Breast ◽  
Breast Meat ◽  
White Striping ◽  
Severe Degree ◽  
Chicken Breast Meat ◽  
Severity Degree

The aim of this study was to characterize the breast meat from broilers affected by the white striping myopathy and examine its influence on meat quality. 180 breast samples from male broilers, slaughtered at 42-d-old, were used. Samples were classified according to the severity degree of apparent white stripes on the Pectoralis major muscle surface (“normal” [n=60], “moderate” [n=60], or “severe” [n=60]). Birds affected by the myopathy produced breast meat with higher L*, a* and b* values on the outer surface. Cooking loss decreased as the severity degree increased (30.94% [normal] to 21.65% [severe]). Toughness increased in the samples classified as moderately and severely affected. There was also an increase in the concentrations of lipids (1.60% [normal] to 2.57% [affected samples], on average), cholesterol (84.60 mg/100 g [normal] to 91.73 mg/100 g [severe degree]) and collagen as the severity degree increased. Although there are statistical differences for some evaluated parameters and, even so, these results are considered numerically normal based on previously published literature, we emphasize that the white striping abnormality alters chicken breast meat quality, especially regarding to the protein and fat concentrations, which can offer consumers products with nutritional quality different from the average specified on the packaging.

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