Analyses of Morphological and Morphometrical Deviations of Bull Spermatozoa by Computer Assisted Semen Analysis Technique

2007 ◽  
Vol 2 (4) ◽  
pp. 196-204 ◽  
Author(s):  
M.N. Sundararam ◽  
J. Kalatharan ◽  
K. Thilak Pon Jawahar
Keyword(s):  
Semen Analysis ◽  
Computer Assisted ◽  
Analysis Technique

scholarly journals Proper use and impact of ‘Computer Assisted Semen Analysis’ technique on semen evaluation of farm animals

2017 ◽  
Vol 64 (4) ◽  
pp. 267 ◽  
Author(s):  
I. A. MICHOS (H.Α. ΜΙΧΟΣ) ◽  
A. G. BASIOURA (Α.Γ. ΜΠΑΣΙΟΥΡΑ) ◽  
C. M. BOSCOS (Κ.M. ΜΠΟΣΚΟΣ) ◽  
I. A. TSAKMAKIDIS (Ι.Α. ΤΣΑΚΜΑΚΙΔΗΣ)
Keyword(s):  
Health Management ◽  
Semen Analysis ◽  
Animal Health ◽  
Evaluation Process ◽  
Field Conditions ◽  
Farm Animals ◽  
Computer Assisted ◽  
Factors Affecting ◽  
Analysis Technique ◽  
The Impact

Objective of this review is to present the use of ‘Computer Assisted Semen Analysis’ technique application in farm animal health management, in particular the steps for accurate semen evaluation and the impact of the method in predicting male animal fertility under field conditions. Requirements for proper use of the equipment, factors affecting the evaluation process and the role of the estimated parametres for fertility under field conditions are described. Special reference is made in sperm motility evaluation. It is concluded that the method is an effective and efficient tool for semen evaluation, provided good practices are strictly applied and adhered to, by means of which valid results may be obtained.

Влияние биологической полноценности спермы быков-производителей канадской селекции на репродуктивные показатели коров отдельного стада

2017 ◽  
pp. 18-21
Author(s):  
Александр Александрович Некрасов ◽  
Николай Александрович Попов ◽  
Байлар Садраддинович Иолчиев
Keyword(s):  
Semen Analysis ◽  
Computer Assisted

Исследовано влияние качества спермы быков-производителей на репродуктивные показатели стада. Материалом для исследования послужила замороженно-оттаянная сперма быков-производителей канадской селекции (n = 6), которую использовали для осеменения коров и телок на ферме «Дубровицы» ФГУП «Кленово-Чегодаево». Концентрацию и подвижность сперматозоидов оценивали с помощью компьютерной технологии Computer-assisted semen analysis (CASA). Состояние хроматина в сперматозоидах изучали методом акридин-оранжевого теста с флуоресцентной микроскопией. Морфологию и целостность акросом оценивали с использованием Дифф-Квик окраски. Всего на ферме «Дубровицы» плодотворно были осеменены 161 корова и 29 телок, от них получены 180 телят, из них 97 бычков и 83 телки. Установлено, что одним из биотических факторов, влияющих на репродуктивные показатели, является состояние ДНК в хроматине. От степени фрагментации ДНК в хроматине сперматозоидов зависит их оплодотворяющая способность и другие показатели фертильности быков-производителей. Уровень воспроизведения обусловлен репродуктивным потенциалом как коров, так и быков-производителей. Для комплексной оценки качества спермы быков-производителей необходимо использовать компьютерные технологии с привлечением максимального числа показателей, в том числе наследственного материала, содержащегося в сперматозоидах. Целесообразно при оценке быков-производителей вести учет благополучия отелов, выбытия коров после патологических отелов, абортов, мертворождаемости. Благополучие отелов способствует увеличению сроков последующего производственного использования коров, осемененных спермой быков-производителей, характеризующихся легкими отелами. Для достоверной оценки биологической полноценности спермы необходимо использовать дополнительный показатель, а именно степень фрагментации ДНК в хроматине. У быков-производителей с высокой степенью фрагментации ДНК получены низкие результаты оплодотворяемости.

Effect of separate and combined exposure of selenium and diazinon on rat sperm motility by computer assisted semen analysis

2016 ◽  
Vol 38 ◽  
pp. 144-149 ◽  
Author(s):  
Robert Toman ◽  
Svatoslav Hluchy ◽  
Michal Cabaj ◽  
Peter Massanyi ◽  
Shubhadeep Roychoudhury ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Combined Exposure

Cysteamine supplementation revealed detrimental effect on cryosurvival of buffalo sperm based on computer-assisted semen analysis and oxidative parameters

2017 ◽  
Vol 177 ◽  
pp. 56-64 ◽  
Author(s):  
Dheer Singh Swami ◽  
Pradeep Kumar ◽  
R.K. Malik ◽  
Monika Saini ◽  
Dharmendra Kumar ◽  
...  
Keyword(s):  
Detrimental Effect ◽  
Semen Analysis ◽  
Computer Assisted

scholarly journals iTRAQ-Based Sperm Proteomic Analyses Reveals Candidate Proteins Affecting the Quality of Spermatozoa From Boar on Plateaus

2020 ◽  
Author(s):  
Yanling Zhao ◽  
Yaomei Wang ◽  
Feipeng Guo ◽  
Bo Lu ◽  
Jiale Sun ◽  
...  
Keyword(s):  
Western Blot ◽  
Cellular Response ◽  
Sperm Quality ◽  
Semen Analysis ◽  
Estrogen Signaling ◽  
Glutathione Metabolism ◽  
Computer Assisted ◽  
Expression Levels ◽  
Relative Expression

Abstract Background: Tibetan pigs (TP) exhibit heritable adaptations to their hypoxic environments as a result of natural selection. Whereas, what candidate proteins affecting the sperm quality of boar on plateaus has not been clearly investigated yet. Methods: In this study, to reveal the candidate proteins affecting the quality of spermatozoa from boar on plateaus, we analyzed the sperm quality by Computer-assisted semen analysis (CASA) system and Reactive oxygen species (ROS) levels, compared the sperm proteomes between TP and Yorkshire pigs (YP) raised at high altitudes using the isobaric tags for relative and absolute quantitation (iTRAQ) in combination with the liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic method, and confirmed the relative expression levels of the four proteins by western blot. Results: The sperm quality of the TP was superior to that of the YP on plateaus. Of 1,555 quantified proteins, 318 differentially expressed proteins (DEPs) were identified. Gene ontology (GO) analysis revealed that the DEPs were predominantly associated with the sorbitol metabolic process, removal of superoxide radicals, cellular response to superoxide, response to superoxide and regulation of the mitotic spindle assembly. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mainly enriched in pathways involved in the regulation of the actin cytoskeleton, glutathione metabolism, oxidative phosphorylation, and estrogen signaling. And based on the protein-protein interaction (PPI) network analysis, we identified 8 candidate proteins (FN1, EGF, HSP90B1, CFL1, GPX4, NDUFA6, VDAC2, and CP) that might play important roles that affect the sperm quality of boar on plateaus. Moreover, the relative expression levels of the proteins (CFL1, EGF, FN1, and GPX4) were confirmed by western blot. Conclusions: Our results reveal 8 candidate proteins (FN1, EGF, HSP90B1, CFL1, GPX4, NDUFA6, VDAC2, and CP) affecting the sperm quality of boar on plateaus, providing a reference for studies on improving sperm quality and the molecular breeding of boar on plateaus.

P–100 Metabolic, hormonal, and inflammatory status in sub-fertile men with obesity and diabetes

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Abbasihormozi ◽  
A Kouhkan ◽  
A Shahverdi ◽  
A Parhizkar ◽  
Z Zolfaghary ◽  
...  
Keyword(s):  
Semen Analysis ◽  
Metabolic Changes ◽  
Semen Parameters ◽  
Computer Assisted ◽  
Obesity And Diabetes ◽  
Hormonal Dysfunction ◽  
Hs Crp ◽  
Type 2 Diabetic

Abstract Study question To evaluate the association between sperm functionality parameters and biochemical, hormonal, and inflammatory indices in obese and diabetic men. Summary answer Metabolic changes,hormonal dysfunction,and the presence of inflammatory mediators might be considered possible mechanisms in the development of sub-fertility in obese and diabetic sub-fertile men What is known already Although the higher prevalence of subfertility in obese and diabetic men during the reproductive age is evident, the mechanisms by which obesity and diabetes mellitus (DM) cause male infertility are not entirely understood. Several pathways might be involved in the role of obesity in semen quality, thereby inducing alterations in hormonal profiles, abnormal lipid metabolism, and possibly the formation of inflammatory cytokines, ultimately leading to impaired sperm function Study design, size, duration We enrolled normal weight (BMI<25 kg/m2) and non-type–2 diabetic (control=40), obese and non- type–2 diabetic (obese=40), non-obese and type–2 diabetic (Lean-DM=35), and obese and type–2 diabetic (Obese-DM=35) sub-fertile men, aged 20–50 years, referring to Royan infertility clinic (Tehran, Iran) from March to September 2014 Participants/materials, setting, methods After enrollment and receiving informed consent, all men underwent face-to-face private interviews. The obesity-associated markers, insulin resistance, beta-cell function, hormonal and lipid profile, inflammatory indices, and semen analysis were assessed in four experimental groups. Semen analysis was examined after 2–5 days of sexual analysis).abstinence based on WHO-recommended methods by CASA system (computer-assisted sperm Main results and the role of chance Main results and the role of chance: Our finding showed that diabetic markers were significantly increased in two diabetic groups, while obesity indices were markedly increased in two obese groups. Conventional sperm parameters were significantly lower in obese DM, lean DM, and obese groups compared with the control (p < 0.05). Serum levels of total testosterone (TT) and sex hormone-binding globulin (SHBG) were significantly lower in men with obesity and DM compared with the control (p < 0.05).There was a significant difference in the concentration of high-sensitivity C-reactive protein (hs-CRP) among four experimental groups. Moreover, serum leptin was significantly increased in obese DM, lean DM, and obese groups. Serum insulin levels had a positive correlation with metabolic-associated indices (WC, BMI, FBS,HbA1c,and HOMA-IR), as well as hs-CRP levels, whereas it had a negative correlation with count, motility, and morphology. There is also a negative association between metabolic-associated indices (WC, BMI, FBS, HbA1c, and HOMA-IR) and semen parameters. Limitations, reasons for caution It was better to evaluate inflammatory biomarkers be examined in other tissues Wider implications of the findings: The results of this study demonstrated the association of metabolic changes, hormonal dysfunction, and inflammatory responses with the semen parameters of sub-fertile men with obesity and diabete. Trial registration number Not applicable

Cryopreservation of collared peccary (Pecari tajacu L., 1758) epididymal sperm using extenders based on Tris and powdered coconut water (ACP®-116c)

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 301-307 ◽  
Author(s):  
José A. B. Bezerra ◽  
Andréia M. Silva ◽  
Patrícia C Sousa ◽  
Lívia B. Campos ◽  
Érica C. G. Praxedes ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Semen Analysis ◽  
Membrane Integrity ◽  
Egg Yolk ◽  
Coconut Water ◽  
Computer Assisted ◽  
Epididymal Sperm ◽  
Sperm Plasma Membrane ◽  
Pecari Tajacu ◽  
Functional Membrane

SummaryThe aim of this study was to establish a functional freezing–thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed. In addition, to evaluate the survival of frozen–thawed sperm, a thermal resistance test (TRT) was executed. Samples preserved using Tris were in better condition compared with those preserved using ACP®, showing higher values for most assessments performed, including CASA and the TRT (P<0.05). After determining Tris to be the better of the two extenders, additional samples were thawed using different thawing rates (37°C/60 s, 55°C/7 s, 70°C/8 s). Sperm thawed at 37°C/60 s had the greatest preservation (P<0.05) of viability (54.1 ± 5.9%) and functional membrane integrity (43.2 ± 5.4%), and had higher values for various CASA parameters. In conclusion, we suggest the use of a Tris-based extender added to egg yolk and glycerol for the cryopreservation of epididymal sperm obtained from collared peccaries. In order to achieve better post-thawing sperm quality, we suggest that samples should be thawed at 37°C/60 s.

86 MOTILITY AND VIABILITY OF BULL SEMEN DURING 24 HOURS OF STORAGE AT 4°C

2006 ◽  
Vol 18 (2) ◽  
pp. 151
Author(s):  
M. E. Carini ◽  
R. Cavia ◽  
G. Larraburu ◽  
G. M. Brogliatti
Keyword(s):  
Storage Time ◽  
Semen Analysis ◽  
Computer Assisted ◽  
Bull Semen ◽  
Multiple Comparison Test ◽  
Straight Line ◽  
Semen Extender ◽  
One Way Anova ◽  
Velocity Average ◽  
Lateral Head

Currently, cryopreservation process of fresh bull semen is carried out between 3 and 6 hours of refrigeration at 4°C post-collection (Hafez, 1989). However, it is sometimes difficult when the cryopreservation process is not available at the site of collection. The objective of this study was to determine seminal motility and viability in samples maintained at 4°C during 24 hours. A total of 98 ejaculates from 23 adult bulls (Angus, Brangus, Braford and Hereford) were collected and diluted in a semi-defined semen extender (Andromed, Minitüb, Tiefenbach, Germany) and stored at 4°C. Parameters of velocity average path (VAP, µm/s), velocity straight line (VSL, μm/s), amplitude lateral head (ALH, μm), linearity (LIN, %), percentage of rapid cells (RAPID, %), percentage of slow and static cells (SL/ST, %), and viability (VIA, %) were determined by Computer Assisted Semen Analysis (CASA, HTM-ceros 12.1, Berkeley, CA, USA). Measurements were done at 6, 9, 12, and 24 h. The obtained results were analyzed statistically with one-way ANOVA and Dunnet Multiple Comparison Test and are summarized in Table 1. There were no significant differences (P > 0.05) in the VAP, RAPID, or SL/ST during 24 h of storage at 4°C. Measurements were significantly different (P < 0.01) for VSL and VIA at 24 h. Measurements of ALH were increased from 12 h (P < 0.01) and consequently, LIN decreased at the same time (P < 0.01). These results suggest that there are no differences in velocity, except in VSL at the end of the storage time. The type of movement of the spermatozoa change, because ALH increases and the trajectory loses linearity. A decrease in viability suggests that from 24 h of storage, the membrane of the spermatozoa becomes more susceptible. More research needs to be done to evaluate the competence of this time-storage semen in the artificial insemination trial. Table 1. Parameters of motility and viability of semen maintained at 4°C during 24 h This research was supported by Centro Genético Bovino de EOLIA S.A.

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