GnRH at Time of Insemination of Sexed Semen Enhanced Conception Rate in Holstein Heifers

This study was undertaken at the calf rearing project (CRP) of AMUL at Sarsa Village, Anand, Gujarat to assess the conception rate and sorting efficiency of imported sexed semen of HF bulls. In all, 127 pubertal crossbred heifers (b.wt. 250-300 kg) were artificially inseminated during the year 2016-17 using 172 sexed semen doses (few with repeat insemination), resulting in 68 pregnancies. The conception rate of sexed semen was found to be 39.53%. Out of 68 pregnant heifers, 2 heifers aborted and one heifer died before calving. Out of rest 65 animals that calved, 54 heifers delivered female calves, 9 had male calves and two were female stillbirth. Thus, sexed semen produced 86.15% female calves and 13.85 % male calves. This small scale study highlights the success of sexed semen under field condition in producing calves of the desired sex in high percentage.

Download Full-text

368 CONCEPTION RATE AT FIRST ARTIFICIAL INSEMINATION WITH SEXED SEMEN OR CONVENTIONAL SEMEN IN CROSSBRED DAIRY COWS

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab368 ◽

2010 ◽

Vol 22 (1) ◽

pp. 340

Author(s):

B. T. Gerhardt ◽

L. D. P. Sinedino ◽

A. P. Dourado ◽

P. A. M. Alves ◽

L. A. G. Nogueira

Keyword(s):

Dairy Cows ◽

Milk Production ◽

Genetic Selection ◽

Conception Rate ◽

Control Group ◽

Chi Square ◽

Fertility Reduction ◽

Estrus Detection ◽

Sexed Semen ◽

Experimental Group

The use of sexed semen in dairy cattle almost eliminates the birth of unwanted male calves and can lead to increased milk production by means of genetic selection exerted on the females. However, AI with sexed semen still presents several obstacles to overcome. Holstein-Zebu cows are responsible for 80% of the milk produced in Brazil. These crossbred animals are used extensively throughout the country because their physiologic and morphologic characteristics are compatible with milk production in the tropics which results in an economic performance advantage. The objective of this study is to evaluate the conception rates with sexed semen, comparing it to the use of non-sorted semen in crossbred dairy cows. In addition, two insemination times had been studied to determine the best timing for AI with the sexed semen. Both studies were carried out in the Experimental Station Seropedica of Pesagro-RJ, located in the state of Rio de Janeiro, Brazil. The experiment was done in 2008/2009 (January to April). In the first Trial 56 Holstein-Zebu females, composed of 1/2 to 7/8 Holstein genetics, were inseminated 12 h after estrus detection with non-sorted semen (control group n = 29, 14 heifers and 15 cows) or with sexed semen (experimental group n = 27, 13 heifers and 14 cows). The semen utilized was from the same bull and thawing was performed intravaginally. In the second trial, 55 females were inseminated only with sexed semen from the same bull, with thawing in water at 35°C, at two different times: 12 h after estrus observation (control group n = 27, 12 heifers, 4 primiparous, and 11 multiparous) and 15 h after estrus observation (experimental group n = 28, 12 heifers, 5 primiparous, and 11 multiparous). The pregnancy diagnoses were done by rectal palpation and transrectal CAT scan at Day 45. Statistical analyses had been done using Chi-square. The conception rate in the first trial was of 17.2% (5/29) for sexed semen and 53.8% (14/26) for non-sorted semen (P < 0.05). In the second trial, the conception rate was of 37.0% (10/27) for insemination with sexed semen, 12 h after estrus observation and 50.0% (14/28) for the females inseminated 15 h after estrus observation (P > 0.05). It is possible that one of the causes of fertility reduction with sexed semen is that sexed-sperm remain viable for a shorter time after the insemination. Inseminations with sexed semen done 15 h after estrus detection, which can be more synchronized with the time of ovulation, and when accompanied by closely controlling the thawing process, show results similar to those obtained with conventional semen.

Download Full-text

Involuntary reduction in vigour of calves born from sexed semen

Acta Veterinaria Hungarica ◽

10.1556/004.2016.023 ◽

2016 ◽

Vol 64 (2) ◽

pp. 229-238 ◽

Cited By ~ 5

Author(s):

Radica Djedović ◽

Vladan Bogdanović ◽

Dragan Stanojević ◽

Zsolt Nemes ◽

András Gáspárdy ◽

...

Keyword(s):

Birth Weight ◽

Sex Ratio ◽

Artificial Insemination ◽

Reproductive Traits ◽

Conception Rate ◽

Gestation Length ◽

Stillbirth Rate ◽

Sexed Semen ◽

The Mean ◽

Fertility Traits

The objective of this study was to compare the reproductive traits of heifers and the development characteristics of their calves following artificial insemination (AI) with sexed and non-sexed semen. The analysed characteristics included conception rate, gestation length, calf birth weight, calf vigour, stillbirth rate, and twinning rate. Data of 530 calves produced with sexed and 1,163 calves produced with non-sexed semen were analysed. The General Linear Model (GLM) was applied to assess the influence of semen type, farm, season of insemination, the calf’s sex and the inseminating sire on gestation length and calf birth weight. With the exception of gestation length (P > 0.05), all other traits studied were significantly (P < 0.01) influenced by the type of semen. The conception rate was 55% for conventional and 44% for sexed semen, and the average gestation length was 274.6 and 274.9 days, respectively. The mean calf birth weight was 37.47 kg for non-sexed and 36.75 kg for sexed semen. The stillbirth rate was 6.19% for conventional and 7.54% for sexed semen, while the twinning rate was 3.78% for conventional and 1.13% for sexed semen. The calves produced with non-sexed and sexed semen differed significantly in viability (P < 0.001), the latter having a lower calf vigour score. The use of conventional semen did not affect the ratio of female and male calves (52.7:47.3%; P > 0.05); however, artificial insemination with X-sorted sexed semen significantly altered the sex ratio of calves (85.1:14.9%, P < 0.01). The results obtained in this investigation are in agreement with the majority of studies which compared the fertility traits, sex ratio and calf characteristics depending on the application of artificial insemination with sexed or conventional semen.

Download Full-text

Modern biotechnological methods in the reproduction of dairy cattle

Glavnyj zootehnik (Head of Animal Breeding) ◽

10.33920/sel-03-2006-03 ◽

2020 ◽

pp. 19-26

Author(s):

S. Abugaliev ◽

L. Bupebaeva ◽

M. Baybatyrova ◽

N. Matkerimov ◽

K. Matkerimova ◽

...

Keyword(s):

Point Of View ◽

Conception Rate ◽

Fetal Mortality ◽

Selection Methods ◽

Cattle Breeding ◽

Sexed Semen ◽

Uterine Mucosa ◽

The Republic ◽

Efficiency Of Selection ◽

Ultrasound Scanner

The problem of increasing the highly productive number of dairy cows is becoming a priority in the cattle breeding of the Republic of Kazakhstan. The decrease in conception rate of cattle is associated with frequently detected in animals functional disorders of the reproductive system, including violations of the functional activity of the ovaries, manifested in the form of their hypofunction or cysts and inflammation of the uterine mucosa. The research has been carried out within the framework of budget projects of the Ministry of agriculture of the Republic of Kazakhstan: “Transfer and adaptation of technologies for automation of technological processes of livestock production on the basis of model farms in dairy farming from 100 cows from different regions of the Republic of Kazakhstan” and “Improving the efficiency of selection methods in cattle breeding” in the peasantry farm “Aidarbayev E. S.” Enbekshikazakh district in the Almaty region in the Republic of Kazakhstan. It has been found according to the results of the research that the first signs of pregnancy ultrasound scanner detects at least 26 days, but the forecast is more accurate at the period of at least 33 days (according to 5 indicators). During the studied period in the peasantry farm “Aidarbayev” from 483 heads maternal livestock 359 heads of calves have been obtained or 74,3 %. Out of the total number of calves 251 alive calves have been received or 69,9 %, and 24,5 % of stillbirths. Of the calved 27 heads of cows 18 heifers or 66,7 % have been received, which is quite effective from a practical point of view. The sexed semen, which has being obtained in recent years, the percentage of conception rate from the first insemination has increased. Further research will focus on the causes of fetal mortality of calves and increased of conception rate when using sexed semen.

Download Full-text

Effects of use of conventional and sexed semen on the conception rate in heifers: A comparison study

Theriogenology ◽

10.1016/j.theriogenology.2019.06.012 ◽

2019 ◽

Vol 135 ◽

pp. 33-37 ◽

Cited By ~ 2

Author(s):

Kohei Oikawa ◽

Takeshi Yamazaki ◽

Satoshi Yamaguchi ◽

Hayato Abe ◽

Hanako Bai ◽

...

Keyword(s):

Conception Rate ◽

Comparison Study ◽

Sexed Semen

Download Full-text

40 TIMING OF INSEMINATION WITH SEXED OR NONSEXED SEMEN ON PREGNANCY RATES OF JERSEY HEIFERS DETECTED IN HEAT BY RADIOTELEMETRY

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab40 ◽

2010 ◽

Vol 22 (1) ◽

pp. 178 ◽

Cited By ~ 1

Author(s):

J. N. S. Sales ◽

G. A. Crepaldi ◽

M. Fosado ◽

E. P. Campos Filho ◽

P. S. Baruselli

Keyword(s):

Pregnancy Rate ◽

Detection System ◽

Pregnancy Rates ◽

Conception Rate ◽

Follicle Diameter ◽

Ovulatory Follicle ◽

Estrus Detection ◽

Sexed Semen ◽

Freezing Procedure ◽

Follicular Dynamics

The objectives of this study were to evaluate the follicular dynamics (experiment 1) and the effects of the timing of insemination with sexed or nonsexed semen on pregnancy rates (experiment 2) of Jersey heifers detected in heat by a radiotelemetric estrus detection system. In experiment 1, 43 Jersey heifers, around 12 mo old and BCS of 2.68 ± 0.11 (1 to 5 scale) were used. The Heat Watch (HW) system was utilized to detect the onset of estrus and mounting behavior associated with estrus. Ultrasound examinations to monitor follicular dynamics occurred every 12 h from estrus onset for 48 h. Statistical analyses were performed using GLM and GLIMMIX procedure of SAS (SAS Institute, Cary, NC, USA). The results of experiment 1 indicated a mean ovulatory follicle diameter of 14.1 ± 0.3 mm, ovulation rate of 86.1% (37/43), and an interval of 31.2 ± 0.9 h from onset of heat to ovulation. In experiment 2, 753 Jersey heifers were allocated in a 2 × 4 factorial with semen (sexed and nonsexed) and AI period (0 to 6, 6 to 12, 12 to 18, and 18 to 24 h after heat onset) as parameters. Semen from 3 bulls was used, with ejaculates divided in 2 fractions: one fraction was submitted to the traditional freezing procedure and the other was submitted to the sexing process and then frozen. The statistical analysis was performed using GLIMMIX procedure of SAS. There was no interaction among the semen, bull, and AI period. There were effects on pregnancy rate by type of semen [sexed 49.5% (189/382) and nonsexed 64.2% (238/371); P = 0.001] and by bull [bull A 53.5% (107/200)b, bull B 50.0% (108/216)b and bull C 63.4% (211/333)a; P = 0.008]. Semen from bull C resulted in a greater pregnancy rate for both sexed and nonsexed semen. Within semen type, there were no differences in pregnancy rates by AI moment [sexed: 0 to 6 h 48.2% (41/85), 6 to 12 h 48.7% (54/111), 12 to 18 h 49.5% (49/99), 18 to 24h 52.4% (44/84) and nonsexed: 0 to 6h 62.8% (49/78), 6 to 12h 60.6% (63/104), 12 to 18h 68.0% (68/100), 18 to 24h 64.8% (57/88); P = 0.77]. We conclude that the use of sexed semen resulted in a lower pregnancy rate than nonsexed semen, and that AI timing does not affect conception rate in Jersey heifers identified in estrus by radiotelemetric estrus detection system. However, there was a bull effect on conception rate. The authors wish to thank Sexing Technologies and Dalhart Jersey Ranch.

Download Full-text

Conception rate following artificial insemination with sexed semen in Holstein heifers under artificial cooling during summer compared with winter season

Tropical Animal Health and Production ◽

10.1007/s11250-019-01998-9 ◽

2019 ◽

Vol 52 (1) ◽

pp. 203-209

Author(s):

Abelardo Correa-Calderón ◽

Ismael Angulo-Valenzuela ◽

Fernando Betancourth ◽

Francisco Oroz-Rojo ◽

Karina Fierros-Castro ◽

...

Keyword(s):

Artificial Insemination ◽

Winter Season ◽

Conception Rate ◽

Sexed Semen

Download Full-text

The New Method for Increasing the Genetic Potential in a Nucleus of Romanian Buffaloes, by Unicornual Artificial Insemination with Sexed Semen, after Stimulation with OvSynch Protocol

10.20944/preprints202108.0106.v1 ◽

2021 ◽

Author(s):

Stefan Ciornei ◽

Dan Drugociu ◽

Liliana Margareta Ciornei ◽

Rosca Petru

Keyword(s):

Artificial Insemination ◽

Conception Rate ◽

Ovarian Activity ◽

Dominant Follicle ◽

Genetic Potential ◽

Gynecological Examination ◽

Sexed Semen ◽

The Moment ◽

Female Cattle ◽

Buffalo Cow

Although artificial insemination can mean a slow and progressive improvement of animal genetics, in buffalo-cow its practical application is difficult the results are incomparably lower then cattle. The purpose of this article is to develop a new methodology, a well-known technique to make AI biotechnology more applicable to the buffalo, in order to improve the conception rate. The protocol we follow is to stimulate hormonal ovarian activity, inducing the dominant follicle, causing ovulation and scheduling the moment of sexed artificial insemination deep in the uterine horn only ipsilateral to the ovary that will ovulate. The experiment was performed on 40 primiparous buffaloes-cows in two groups for AI separated by two bulls whit 2 Millions female sperm straws. The groups was compiled after a thorough gynecological examination, and subsequently the OvSynch therapeutic protocol was started. The results were 82,5% buffaloes (33/40) had dominant follicle (DF) and inseminated, on hot/cold seasons the distribution was 75%vs90%. The conception rate was 63,6% (21/33), on hot/cold seasons 60%vs66,6%, and after calving 92.5% (20/21) female cattle were obtained. Thus, by implementing UcFTAI protocol, we state that the goal of increasing the genetic potential of CIB by becomes achievable and can be extended to a larger scale. Our Improved Protocol (UcFTAI) aims to reduce waste and maximize OvSynch hormone therapy.

Download Full-text