Phytochemical Analysis and in vitro Evaluation of Antidiabetic Activity of Diospyros buxifolia

Medicinal plants possess therapeutic potential and are used to treat various diseases around the world. Eclipta prostrate (L.) is a medicinal herb that has extensive application in the native medicinal system. In any therapeutic activity chemical constituents play an important role. Eclipta prostrata has been investigated in this study for its antioxidant, antimicrobial and antidiabetic activity in vitro. The well-known research protocol available in the literature established qualitative analysis of the different phytochemical constituents and quantitative analysis of total phenol and flavonoids. The hydroalcoholic extracts of the leaves and seeds of Eclipta prostrata exhibited significant and dose-dependent antioxidant activity including ability to donate electron. To analyze the antimicrobial activity, Leaves hydroalcoholic extracts and Eclipta prostrate seeds were tested against two selected strains using a well-diffusion method and showing significant inhibitory action against all the strain tested. In addition, the dose-dependent α-amylase inhibitory activity with an IC50 value of acarbose, leaves, and seed extract was found to be 364.89μg/ml and 438.43μg/ml, respectively, indicating that Eclipta prostrate is a promising source as an herbal medicine. Keywords: Eclipta prostrate, Phytochemical Analysis, Antioxidant, Antimicrobial, Antidiabetic Activity.

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IN VITRO ANTIDIABETIC ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF LUMNITZERA RACEMOSA LEAVES

International Research Journal of Pharmacy ◽

10.7897/2230-8407.1004152 ◽

2019 ◽

Vol 10 (4) ◽

pp. 220-227

Author(s):

Ranjana . ◽

B L Jadhav ◽

Pratik P Dhavan ◽

Preeti Patel

Keyword(s):

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Lumnitzera Racemosa

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EVALUATION OF IN VITRO ANTIOXIDANT AND ANTIDIABETIC POTENTIALS OF DIFFERENT FRACTIONS OF MAYTENUS HEYNEANA ROOT EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.30088 ◽

2019 ◽

Vol 12 (1) ◽

pp. 408

Author(s):

Sumithira G ◽

Senthil Kumar Gp

Keyword(s):

Ethyl Acetate ◽

Radical Scavenging ◽

Cation Radical ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Root Extract ◽

Prevention Of Diabetes

Objective: In an attempt to explore herbal drug which may become useful in the prevention of diabetes and antioxidant potential by the ethanol extracts of Maytenus heyneana (MH) root belonging to the family Celastraceae and their different fractions were studied.Methods: Different fractionation was done using chloroform, ethyl acetate, and methanol on ethanolic extract of MH and preliminary phytochemical analysis was done by standard methods to identify the presence of important compounds. In vitro antioxidants activities were carried by 2,2-diphenyl- 2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging assays. For antidiabetic potential, α-amylase and α-glucosidase enzyme inhibitory studies were carried on different fractions.Results: Phytochemical studies show the presence of alkaloids, flavonoids, phenols, cardiac glycosides, and terpenoids in all fractionations; however, tannins and quinones were present in ethyl acetate fraction and saponins in methanolic fraction. For antioxidant activity, ethyl acetate fraction shows concentration of the sample causing 50% inhibition (IC50) values in 22.31 μg/ml and methanolic fraction shows in 12.82 μg/ml concentrations for DPPH and ABTS radical scavenging assay, respectively. In case of antidiabetic activity, methanolic fraction offered significant result in inhibitory action of α-glucosidase and also for α-amylase assay IC50 (5.28 and 3.14 μg/ml) than other fractions.Conclusion: From the results of our studies, it can be concluded that MH shows antidiabetic and antioxidant values and methanolic fraction of MH could be possessed potential constituents in the prevention of diabetes and antioxidant than other fractions. However, further studies are required to validate.

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Phytochemical Analysis, In Vitro Evaluation of Antioxidant and Free Radical Scavenging Activity of Simarouba glauca Seeds

Advances in Pharmacology and Pharmacy ◽

10.13189/app.2021.090101 ◽

2021 ◽

Vol 9 (1) ◽

pp. 1-8

Author(s):

Haleshappa R. ◽

Sharangouda J. Patil ◽

Siddalinga Murthy K. R.

Keyword(s):

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

In Vitro Evaluation ◽

Simarouba Glauca

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ANTIDIABETIC POTENTIAL OF THE OYSTER MUSHROOM PLEUROTUS FLORIDA (MONT.) SINGER

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2017v9i4.20765 ◽

2017 ◽

Vol 9 (4) ◽

pp. 51

Author(s):

Prabu M. ◽

Kumuthakalavalli R.

Keyword(s):

Inhibitory Activity ◽

Tamil Nadu ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Dependent Manner ◽

Pleurotus Florida ◽

Dose Dependent

Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida.Methods: The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously.Results: In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug.Conclusion: The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent.

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