In vitro Degradation Behavior of Bombyx mori Silk Fibroin Films Exposure to Protease XXIII

Transgenic silk fibroins incorporated the VEGF and RGD were prepared. The VEGF SF showed lower platelet adhesion than the RGD SF and WT SF. An in vivo implantation study supported these in vitro results.

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Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth

Polymers ◽

10.3390/polym13234182 ◽

2021 ◽

Vol 13 (23) ◽

pp. 4182

Author(s):

Chavee Laomeephol ◽

Apichai Vasuratna ◽

Juthamas Ratanavaraporn ◽

Sorada Kanokpanont ◽

Jittima Amie Luckanagul ◽

...

Keyword(s):

Bombyx Mori ◽

Silk Fibroin ◽

Biomedical Applications ◽

Spider Silk ◽

Gelation Time ◽

Cellular Interactions ◽

Spider Silk Protein ◽

Biological Tests ◽

Bombyx Mori Silk

Binary-blended hydrogels fabricated from Bombyx mori silk fibroin (SF) and recombinant spider silk protein eADF4(C16) were developed and investigated concerning gelation and cellular interactions in vitro. With an increasing concentration of eADF4(C16), the gelation time of SF was shortened from typically one week to less than 48 h depending on the blending ratio. The biological tests with primary cells and two cell lines revealed that the cells cannot adhere and preferably formed cell aggregates on eADF4(C16) hydrogels, due to the polyanionic properties of eADF4(C16). Mixing SF in the blends ameliorated the cellular activities, as the proliferation of L929 fibroblasts and SaOS-2 osteoblast-like cells increased with an increase of SF content. The blended SF:eADF4(C16) hydrogels attained the advantages as well as overcame the limitations of each individual material, underlining the utilization of the hydrogels in several biomedical applications.

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Oxygen Permeability of Silk Fibroin Hydrogels and Their Use as Materials for Contact Lenses: A Purposeful Analysis

Gels ◽

10.3390/gels7020058 ◽

2021 ◽

Vol 7 (2) ◽

pp. 58

Author(s):

Traian V. Chirila

Keyword(s):

Tissue Engineering ◽

Bombyx Mori ◽

Silk Fibroin ◽

Biomedical Applications ◽

Oxygen Permeability ◽

Contact Lenses ◽

The Past ◽

Fibrous Protein ◽

Silk Moth ◽

Bombyx Mori Silk

Fibroin is a fibrous protein that can be conveniently isolated from the silk cocoons produced by the larvae of Bombyx mori silk moth. In its form as a hydrogel, Bombyx mori silk fibroin (BMSF) has been employed in a variety of biomedical applications. When used as substrates for biomaterial-cells constructs in tissue engineering, the oxygen transport characteristics of the BMSF membranes have proved so far to be adequate. However, over the past three decades the BMSF hydrogels have been proposed episodically as materials for the manufacture of contact lenses, an application that depends on substantially elevated oxygen permeability. This review will show that the literature published on the oxygen permeability of BMSF is both limited and controversial. Additionally, there is no evidence that contact lenses made from BMSF have ever reached commercialization. The existing literature is discussed critically, leading to the conclusion that BMSF hydrogels are unsuitable as materials for contact lenses, while also attempting to explain the scarcity of data regarding the oxygen permeability of BMSF. To the author’s knowledge, this review covers all publications related to the topic.

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