In vitro Cytotoxic Activity of Sargassum thunbergii and Dictyopteris divaricata (Jeju Seaweeds) on the HL-60 Tumour Cell Line

2009 ◽  
Vol 5 (5) ◽  
pp. 298-306 ◽  
Author(s):  
K.N. Kim ◽  
Y.M. Ham ◽  
J.Y. Moon ◽  
M.J. Kim ◽  
D.S. Kim ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Sargassum Thunbergii

Synthesis of α-Aminoalkyl Phosphonate Derivatives of Resveratrol as Potential Antitumour Agents

2008 ◽  
Vol 61 (6) ◽  
pp. 472 ◽  
Author(s):  
Lei Shi ◽  
Xian-Feng Huang ◽  
Zhen-Wei Zhu ◽  
Huan-Qiu Li ◽  
Jia-Yu Xue ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Tumour Cell Line ◽  
Partial Synthesis ◽  
Antitumour Agents ◽  
Inhibition Concentration ◽  
Ic50 Values ◽  
Epidermoid Tumour ◽  
Derivatives Of

Several α-aminoalkyl phosphonate derivatives of resveratrol were first prepared by partial synthesis from resveratrol. Antitumour activities of the synthesized compounds were determined against a human nasopharyngeal epidermoid tumour cell line KB and a human normal cell line L02 in vitro. The results indicated that these compounds showed good cytotoxic activity against KB but weak cytotoxic activity against L02. Compounds 5c and 5d showed significant cytotoxic activity against KB, with median inhibition concentration (IC50) values of 0.4 μM and 0.9 μM, respectively. On the basis of the biological results, the structure–activity relationship is discussed concisely. The potent antitumour activities shown by 5c and 5d make these resveratrol phosphonate derivatives of great interest for further investigations.

Synthesis, Characterization, and Antibacterial and Cytotoxic Study of Metal Complexes with Schiff Base Ligands

2008 ◽  
Vol 61 (4) ◽  
pp. 288 ◽  
Author(s):  
Lei Shi ◽  
Rui-Qin Fang ◽  
Jia-Yu Xue ◽  
Zhu-Ping Xiao ◽  
Shu-Hua Tan ◽  
...  
Keyword(s):  
Schiff Base ◽  
Metal Complexes ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Primary Amines ◽  
Tumour Cell Line ◽  
Cytotoxic Activities ◽  
Ionization Mass ◽  
Schiff Base Ligands

The synthesis of 16 metal complexes from four Schiff bases prepared from 5-chloro-2-hydroxybenzaldehyde and primary amines has been described. The synthesized Schiff base ligands and their complexes were characterized by elemental analyses, spectroscopic (UV, IR, 1H and 13C NMR, electrospray ionization-mass spectrometry) methods, and magnetic and conductance measurements. Furthermore, complexes 1a, 1b, 3d, 4a, and 4d were characterized by X-ray diffraction analysis. After the structural characterization, all the compounds were tested in vitro for their antibacterial (Bacillus subtilis, Escherichia coli, Pseudomonas fluorescens, and Staphylococcus aureus) activities. The cytotoxic activities of the synthesized compounds were evaluated in vitro against human chronic myeloid leukaemia cells (K562) and a human nasopharyngeal epidermoid tumour cell line. The results indicated that most of the complexes showed good cytotoxic activity against human cancer cell lines but weak cytotoxic activity against a human normal cell line (L02). Among the compounds tested, the cobalt complexes 1a, 2a, 3a, and 4a showed the most favourable antibacterial and cytotoxic activities.

scholarly journals Characterization of bioactive substances MHGF-68 on tumour cell lines with LiveFlow In Vitro Technology

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
R. Hodoši ◽  
E. Nováková ◽  
K. Macková ◽  
M. Molitorisová ◽  
M. Šupolíková
Keyword(s):  
Cell Line ◽  
Cell Morphology ◽  
Tumour Cell ◽  
Tumour Cell Line ◽  
Bioactive Substances ◽  
Dynamic Cultivation ◽  
The Impact ◽  
Cells Cultured

Abstract As part of experimental research, growth factor-like substances associated with MHV-68, named MHGF-68, were discovered in our laboratory. MHGF-68 activity was manifested by the ability to alter cell morphology, that is, normal phenotype to transformed, resp. suppresses the transformed phenotype of tumour cells. The aim of the experiments was to monitor the effect of MHGF-68 on the change of the cell actin cytoskeleton in the tumour cell line Hepa1c1c7, as well as the normal cell line NIH3T3, and compare conventional stationary cultivation and dynamic cultivation conditions using a LiveFlow system (In Vitro Technologies). LiveFlow is an advanced system to test the impact of different compounds on the cell cultures, which allows simulation of in vivo conditions thanks to continuous flow of cultivation medium. MHGF-68 was prepared with the infection of BHK-21 cells with MHV-68 virus under non-permissive conditions (41°C). After dynamic cultivation with MHGF-68, we observed changes in morphology on Hepa1c1c7 cells. In cells cultured in a dynamic environment, we observed more pronounced changes in cell morphology in comparison with cells cultured statically. We observed no changes in the cytoskeletal structures in the NIH 3T3 cell line affected by MHGF-68 in both types of cultivation. The advantage of LiveFlow in comparison to in vivo testing is that the experiments performed in this system are less time and money consuming. Dynamic cultivation in the LiveFlow system is suitable for optimizing experiments before testing substances in vivo.

Cytotoxic Cholestane and Pregnane Glycosides from Tribulus macropterus

2007 ◽  
Vol 62 (5-6) ◽  
pp. 319-325 ◽  
Author(s):  
El-Sayed S. Abdel-Hameed ◽  
Hanan A. El-Nahas ◽  
Eman A. El-Wakil
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Tumour Cell ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Chemical Data ◽  
Tumour Cell Line ◽  
Human Tumour ◽  
Human Tumour Cell Line ◽  
Chromatographic Techniques

The methanol extract of the whole parts of Tribulus macropterus Boiss. (family Zygophyllaceae) showed cytotoxic activity against a human tumour cell line (hepatocyte generation 2, HepG2) (IC50 = 2.9 μg/ml). The n-butanolic fraction obtained from successive fractionation of the methanolic extract exhibited activity against HepG2 (IC50 = 2.6 μg/ml). Therefore, this fraction was subjected to separation using different chromatographic techniques. Five compounds, 1-5, were isolated and identified as: (22S,25S)-16β,22,26-trihydroxy-cholest-4- en-3-one-16-O-β-D-glucopyranosyl-(1→3)-β-D-xylopyranoside (1), (22S,25S)-16β,22,26-trihydroxy- cholest-4-en-3-one-16-O-β-D-glucopyranosyl-(1→3)-β-d-glucopyranoside (2), sucrose (3), d-pinitol (4) and 3β-hydroxy-5α-pregn-16(17)en-20-one-3-O-β-D-xylopyranosyl-(1→2)- [β-d-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-galactopyranoside (5) on the basis of spectroscopic and chemical data. The three steroidal compounds 1, 2 and 5 were also tested against the same cell line HepG2 and their IC50 values were 2.4, 2.2 and 1.1 μg/ml, respectively.

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