Antibacterial, In vitro Lipid per Oxidation and Phytochemical Observation on Achyranthes Bidentata Blume

2007 ◽  
Vol 6 (5) ◽  
pp. 447-451 ◽  
Author(s):  
P. Uma Devi ◽  
S. Murugan ◽  
S. Suja ◽  
S. Selvi ◽  
P. Chinnaswam ◽  
...  
Keyword(s):  
Achyranthes Bidentata

scholarly journals Bidentatide, a Novel Plant Peptide Derived from Achyranthes bidentata Blume: Isolation, Characterization, and Neuroprotection through Inhibition of NR2B-Containing NMDA Receptors

2021 ◽  
Vol 22 (15) ◽  
pp. 7977
Author(s):  
Fei Ding ◽  
Yunpeng Bai ◽  
Qiong Cheng ◽  
Shu Yu ◽  
Mengchun Cheng ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Nmda Receptors ◽  
Hippocampal Neurons ◽  
Receptor Subtype ◽  
Achyranthes Bidentata ◽  
Amino Acid Peptide ◽  
Nmda Current ◽  
Neuroprotective Actions ◽  
In Vitro Effects

Increasing attention is being focused on the use of polypeptide-based N-methyl-d-aspartate (NMDA) receptor antagonists for the treatment of nervous system disorders. In our study on Achyranthes bidentata Blume, we identified an NMDA receptor subtype 2B (NR2B) antagonist that exerts distinct neuroprotective actions. This antagonist is a 33 amino acid peptide, named bidentatide, which contains three disulfide bridges that form a cysteine knot motif. We determined the neuroactive potential of bidentatide by evaluating its in vitro effects against NMDA-mediated excitotoxicity. The results showed that pretreating primary cultured hippocampal neurons with bidentatide prevented NMDA-induced cell death and apoptosis via multiple mechanisms that involved intracellular Ca2+ inhibition, NMDA current inhibition, and apoptosis-related protein expression regulation. These mechanisms were all dependent on bidentatide-induced inhibitory regulation of NR2B-containing NMDA receptors; thus, bidentatide may contribute to the development of neuroprotective agents that would likely possess the high selectivity and safety profiles inherent in peptide drugs.

scholarly journals In vitro clonal propagation of Achyranthes aspera L. and Achyranthes bidentata Blume using nodal explants

2012 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Wesely Edward Gnanaraj ◽  
Johnson Marimuthu@Antonisamy ◽  
RB Mohanamathi ◽  
Kavitha Marappampalyam Subramanian
Keyword(s):  
Clonal Propagation ◽  
Nodal Explants ◽  
Achyranthes Bidentata ◽  
Achyranthes Aspera ◽  
In Vitro Clonal Propagation

scholarly journals Effect ofAchyranthes bidentataBlume on 3T3-L1 Adipogenesis and Rats Fed with a High-Fat Diet

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sang Deog Oh ◽  
Mihyun Kim ◽  
Byung-Il Min ◽  
Gi Soon Choi ◽  
Sun-Kwang Kim ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Adipocyte Differentiation ◽  
Body Weight Gain ◽  
Water Extract ◽  
High Fat ◽  
Achyranthes Bidentata ◽  
Reduced Body ◽  
Differentiation Model

The present study investigated the antiobesity effect ofAchyranthes bidentataBlume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect ofAchyranthes bidentataBlume on adipogenesisin vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days withAchyranthes bidentataBlume at various concentrations (1 to 25 μg/mL) for eight days. We found thatAchyranthes bidentataBlume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration ofAchyranthes bidentataBlume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest thatAchyranthes bidentataBlume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet.

scholarly journals Revealing Microbiome Structure and Assembly Process in Three Rhizocompartments of Achyranthes bidentata Under Continuous Monoculture Regimes

2021 ◽  
Vol 12 ◽  
Author(s):  
Juanying Wang ◽  
Hongmiao Wu ◽  
Linkun Wu ◽  
Ye Liu ◽  
Puleng Letuma ◽  
...  
Keyword(s):  
Network Analysis ◽  
Pathogenic Fungi ◽  
Pcr Analysis ◽  
Bacillus Halodurans ◽  
Positive Interaction ◽  
Achyranthes Bidentata ◽  
Negative Effect ◽  
And Performance ◽  
Important Position

The complex composition and interaction of root-associated microbes are critical to plant health and performance. In this study, we presented a detailed characterization of three rhizocompartment (rhizosphere, rhizoplane, and root) microbiomes of Achyranthes bidentata under different years of consecutive monoculture by deep sequencing in order to determine keystone microorganisms via co-occurrence network analysis. The network analysis showed that multiple consecutive monoculture (MCM, represented 5Y and 10Y) soils generated some distinct beneficial bacterial taxa such as Bacillus, Fictibacillus, Bradyrhizobium, Shinella, and Herbaspirillum. For fungi, Mortierella substituted for Fusarium in occupying an important position in different rhizocompartments under A. bidentate monoculture. Quantitative PCR analysis confirmed a significant increase in Bacillus, Pseudomonas, and Burkholderia spp. The results of the inoculation assay showed that addition of beneficial bacteria Bacillus subtilis 74 and Bacillus halodurans 75 significantly increased the root length and fresh weight of A. bidentata. Furthermore, three types of phytosterones, as the main allochemicals, were identified both in the rhizosphere soil and in culture medium under sterile conditions by LC-MS/MS. When looking at in vitro interactions, it was found that phytosterones displayed a positive interaction with dominant beneficial species (Bacillus amyloliquefaciens 4 and B. halodurans 75) and had a negative effect on the presence of the pathogenic fungi Fusarium solani and Fusarium oxysporum. Overall, this study demonstrated that consecutive monoculture of A. bidentata can alter the bacterial and fungal community by secreting root exudates, leading to recruitment of beneficial microbes and replacement of plant-specific pathogenic fungi with plant beneficial fungi.

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