scholarly journals FALSE-NEGATIVE REACTION DUE TO THE PROZONE PHENOMENON IN PLATELET CROSS-MATCH TESTING BY THE MPHA METHOD: A CASE OF NEONATAL ALLOIMMUNE THROMBOCYTOPENIA (NAIT)

2009 ◽  
Vol 55 (3) ◽  
pp. 386-391
Author(s):  
Chie Nishimura ◽  
Hiroyuki Inaba ◽  
Hiroshi Minami ◽  
Nobuo Araki ◽  
Shion Imoto ◽  
...  
Keyword(s):  
False Negative ◽  
Negative Reaction ◽  
Alloimmune Thrombocytopenia ◽  
Cross Match ◽  
Prozone Phenomenon

scholarly journals Syphilitic Balanitis of Follmann: Laboratory Pitfalls

2020 ◽  
Vol 78 (3) ◽  
pp. 265-268
Author(s):  
Filipa Tavares Almeida ◽  
Filomena Azevedo ◽  
Carmen Lisboa
Keyword(s):  
Infected Patient ◽  
False Negative ◽  
Previous History ◽  
Syphilis Infection ◽  
Flocculation Test ◽  
History Of ◽  
Antibody Titers ◽  
Lattice Network ◽  
Antigen Antibody ◽  
Prozone Phenomenon

We report a case of early syphilis, presenting as balanitis and papular syphilides in an HIV-infected patient, with a previous history of syphilis infection, which demonstrated a false negative VDRL testing due to a prozone phenomenon. This false negative response results from overwhelming antibody titers, which interfere with the proper formation of the antigen-antibody lattice network, necessary to visualize a positive flocculation test.

False‐negative solid‐phase platelet crossmatch results due to prozone phenomenon

Transfusion ◽  
2020 ◽  
Vol 60 (12) ◽  
pp. 3055-3059
Author(s):  
Rachel K. Horton ◽  
Micah D. Zuccarelli ◽  
Laurie L. Wakefield ◽  
Margaret A. DiGuardo ◽  
Manish J. Gandhi ◽  
...  
Keyword(s):  
Solid Phase ◽  
False Negative ◽  
Phase Platelet ◽  
Prozone Phenomenon

HLADRB3*0101 Combined with DRB4*0101 Are Potential Predictor of Neonatal Alloimmune Thrombocytopenia (NAIT) Development

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 2221-2221
Author(s):  
Ophira Salomon ◽  
Ron Loewenthal ◽  
Rivka Kalt ◽  
Nurit Rosenberg ◽  
Rima Dardik ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Conflicts Of Interest ◽  
Medical Center ◽  
Control Group ◽  
Study Group ◽  
Severe Thrombocytopenia ◽  
Hla Antibodies ◽  
Hla Dr ◽  
Alloimmune Thrombocytopenia ◽  
Cross Match

Abstract Abstract 2221 Neonatal alloimmune thrombocytopenia (NAIT) is a life-threatening bleeding disorder in the fetus/ neonate caused by maternal alloantibodies directed against fetal platelet antigens inherited from the father. Of the 21 allonatigens described up to date http://www.ebi.ac.uk/ipd/hpa/table2.html, the human platelet antigen (HPA) −1 defined as a leucine /proline polymorphism at residue 33 in b3 integrin is the immune-dominant antigen leading to severe NAIT. Immunization to HPA-1a occurs only in 10% of HPA-1bb pregnant women, 90% of them carrying histocompatibility leukocyte antigen (HLA) DRB3*0101. The aims of the present study were: 1. to prospectively study whether in addition to HLA DR B3*0101 there other potential HLA DR antigens involved in the immune process. 2. To analyse whether the response to IVIgG treatment correlates with maternal HLA DR genotype 3. to determine whether, anti HLA antibodies in addition to HPA1a antibodies, resulted in a more severe thrombocytopenia. We enrolled 21 pregnant women who had previously given birth to newborns with NAIT due to HPA1 incompatibility and had antibodies directed to their spouses HPA1 phenotype (leucine or proline polymorphism ) at the Sheba Medical Center from May 2001 to January 2011. The control group consisted of 21 women who delivered at term newborns with thrombocytopenia and a diagnosis of NAIT was ruled out. Twelve women of 21 (57%) in the study group carried both HLADRB3*0101 and DRB4*0101phenotype. But in none of the women in the control group such combination was detected. Interestingly is the observation that of 20 women who gave birth once more following IVIgG treatment, four did not respond to IVIgG and 3 (75%) of them harbor both DRB3*0101 and DRB4*0101. When lymphocytotoxic cross match analysis was performed in eighteen couples of the study group seven (39%) of them had positive lymphocytotoxic cross match. However the presence of both HLA and HPA1 antibodies against their partner phenotype did not affect platelets' counts in the newborn. Taken together we postulate that the presence of both HLADRB3*0101 and HLADRB4*0101 in the mother increases the risk of NAIT development when HPA1 incompatibility between the couples is found and can serve as a diagnostic tool. The presence of HLA antibodies against the spouse did not alter response to IgG treatment. Disclosures: No relevant conflicts of interest to declare.

Modified Sutter’s Arginine Dihydrolase Medium for Pseudomonas Speciation

1977 ◽  
Vol 60 (2) ◽  
pp. 311-312
Author(s):  
Sylvia G Cary
Keyword(s):  
Pseudomonas Putida ◽  
Biochemical Characterization ◽  
False Negative ◽  
Bromothymol Blue ◽  
Negative Reaction ◽  
Color Contrast ◽  
Pseudomonas Sp ◽  
Cosmetic Products ◽  
Arginine Dihydrolase

Abstract Sutter’s arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. Bromothymol blue is the indicator for alkalinity in Sutter’s medium. By adding glucose and lowering the pH of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. The modified Sutter’s medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic products. Of the 706 strains belonging to the 5 species isolated from cosmetic products, only one false negative reaction was obtained with a strain of Pseudomonas putida. The medium was also used for the biochemical characterization of 122 Pseudomonas sp. isolates.

Comparative Efficiency of Brilliant Green, Bismuth Sulfite, Salmonella-Shigella, Hektoen Enteric, and Xylose Lysine Desoxycholate Agars for the Recovery of Salmonella from Foods: Collaborative Study

1981 ◽  
Vol 64 (4) ◽  
pp. 899-928
Author(s):  
Wallace H Andrews ◽  
Paul L Poelma ◽  
Clyde R Wilson ◽  
◽  
C Abeyta ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
False Positive ◽  
Relative Efficiency ◽  
Brilliant Green ◽  
Collaborative Study ◽  
False Negative ◽  
Reaction Rates ◽  
Negative Reaction ◽  
Comparative Efficiency ◽  
False Positive Reactions

Abstract The relative efficiency of brilliant green (BG), bismuth sulfite (BS), Salmonella-Shigella (SS), xylose lysine desoxycholate (XLD), and Hektoen enteric (HE) agars for the recovery of Salmonella from 5 foods was collaboratively studied in 11 laboratories. The analytical efficiency of various paired combinations of the 5 agars was statistically compared according to 3 parameters: (1) productivity or recovery of Salmonella, (2) rate of enumeration of cultures that were false positive for Salmonella, and (3) rate of enumeration of false-negative reactions. In descending order of productivity, the sequential rankingwasBS, XLD, HE, BG, and SS agars. In ascending order, the rates of false-positive reactions based on a statistical analysis of paired agar combinations was HE, BS, BG and XLD (tie), and SS agars. Analogously, in ascending order, the sequence of false-negative reaction rates was BS, XLD, HE, BG, and SS agars. The combination of BS, XLD, and HE agars is more efficient for recovery of Salmonella from foods than is the present official combination of BG, BS, and SS agars. The revision of official final action method 46.054 to replace the combination of BG, BS, and SS agars with a combination of BS, XLD, and HE agars has been adopted official first action.

False‐negative ELISA result for HIV antibody due to an apparent prozone phenomenon

1987 ◽  
Vol 147 (1) ◽  
pp. 42-42 ◽  
Author(s):  
Allison Imrie ◽  
Susan Best ◽  
Ronald Penny ◽  
David A. Cooper
Keyword(s):  
False Negative ◽  
Prozone Phenomenon ◽  
Elisa Result
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