scholarly journals Cytotoxic effector cells specific for Epstein-Barr nuclear antigen positive nasopharyngeal hybrid cells are present in patients with infectious mononucleosis.

1988 ◽  
Vol 91 (11) ◽  
pp. 1831-1836
Author(s):  
MITSURU FURUKAWA ◽  
MICHIHIRO KAMIDE ◽  
CHIIKO IKEDA ◽  
HIDEO SAKASITA ◽  
RYOZO UMEDA
Keyword(s):  
Infectious Mononucleosis ◽  
Nuclear Antigen ◽  
Hybrid Cells ◽  
Effector Cells ◽  
Epstein Barr ◽  
Epstein Barr Nuclear Antigen ◽  
Cytotoxic Effector

scholarly journals Autoantibodies in infectious mononucleosis have specificity for the glycine-alanine repeating region of the Epstein-Barr virus nuclear antigen.

1987 ◽  
Vol 165 (4) ◽  
pp. 1026-1040 ◽  
Author(s):  
G Rhodes ◽  
H Rumpold ◽  
P Kurki ◽  
K M Patrick ◽  
D A Carson ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Normal Tissue ◽  
Molecular Mimicry ◽  
Nuclear Antigen ◽  
Autoantibody Production ◽  
Barr Virus ◽  
Virally Encoded ◽  
Epstein Barr ◽  
Epstein Barr Nuclear Antigen ◽  
Selection Of

Viruses have been postulated to be involved in the induction of autoantibodies by: autoimmunization with tissue proteins released by virally induced tissue damage; immunization with virally encoded antigens bearing molecular similarities to normal tissue proteins; or nonspecific (polyclonal) B cell stimulation by the infection. Infectious mononucleosis (IM) is an experiment of nature that provides the opportunity for examining these possibilities. We show here that IgM antibodies produced in this disease react with at least nine normal tissue proteins, in addition to the virally encoded Epstein-Barr nuclear antigen (EBNA-1). The antibodies are generated to configurations in the glycine-alanine repeat region of EBNA-1 and are crossreactive with the normal tissue proteins through similar configurations, as demonstrated by the effectiveness of a synthetic glycine-alanine peptide in inhibiting the reactions. The antibodies are absent in preillness sera and gradually disappear over a period of months after illness, being replaced by IgG anti-EBNA-1 antibodies that do not crossreact with the normal tissue proteins but that are still inhibited by the glycine-alanine peptide. These findings are most easily explained by either a molecular mimicry model of IgM autoantibody production or by the polyclonal activation of a germline gene for a crossreactive antibody. It also indicates a selection of highly specific, non-crossreactive anti-EBNA-1 antibodies during IgM to IgG isotype switching.

Immunogenicity study of engineered ferritins with C- and N-terminus insertion of Epstein-Barr nuclear antigen 1 epitope

Vaccine ◽  
2021 ◽  
Author(s):  
Yiran Qu ◽  
Bingyang Zhang ◽  
Yingli Wang ◽  
Shuang Yin ◽  
Jordan L. Pederick ◽  
...  
Keyword(s):  
Nuclear Antigen ◽  
N Terminus ◽  
C And N ◽  
Epstein Barr ◽  
Epstein Barr Nuclear Antigen ◽  
Immunogenicity Study

HAUSP/USP7 as an Epstein–Barr virus target

2004 ◽  
Vol 32 (5) ◽  
pp. 731-732 ◽  
Author(s):  
M.N. Holowaty ◽  
L. Frappier
Keyword(s):  
Epstein Barr Virus ◽  
Latent Infection ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
High Affinity ◽  
Cellular Immortalization ◽  
Epstein Barr ◽  
Epstein Barr Nuclear Antigen

USP7 (also called HAUSP) is a de-ubiquitinating enzyme recently identified as a key regulator of the p53–mdm2 pathway, which stabilizes both p53 and mdm2. We have discovered that the Epstein–Barr nuclear antigen 1 protein of Epstein–Barr virus binds with high affinity to USP7 and disrupts the USP7–p53 interaction. The results have important implications for the role of Epstein–Barr nuclear antigen 1 in the cellular immortalization that is typical of an Epstein–Barr virus latent infection.

Epstein-Barr Virus Infectious Mononucleosis in Children

PEDIATRICS ◽  
1985 ◽  
Vol 75 (6) ◽  
pp. 1011-1019 ◽  
Author(s):  
Ciro Valent Sumaya ◽  
Yasmin Ench
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Immunoglobulin M ◽  
Antibody Responses ◽  
Adult Patients ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Age Related ◽  
Slide Test ◽  
Epstein Barr

An investigation was performed to address the need to establish the rate of positive heterophil antibody responses, oropharyngeal isolation of Epstein-Barr virus (EBV), and the evolving pattern of EBV-specific antibody responses among children with documented EBV-infectious mononucleosis. Findings showed that the rate of heterophil antibody responses appeared to increase progressively with advancing age from infancy up to 4 years, after which the rates approached values similar to that reported in young adult patients. The rapid slide test detected a heterophil antibody response as frequently as the Paul-Bunnell-Davidsohn horse cell test, except in children less than 4 years old. The decreased sensitivity found with the rapid slide test in the very young was associated with their less intense heterophil response. The younger group of children also developed a lower acute mean titer and, as a result, a decreased persistence of immunoglobulin M antibody to EBV-capsid antigen, whereas they had more frequent responses to EBV-early antigen directed to restricted component than both the older subjects and adults reported elsewhere. Antibodies to EBV-nuclear antigen, characteristically a late-onset antibody, tended to develop earlier than noted in adult patients. In contrast, the prevalence and continued excretion of EBV from oropharyngeal secretions was similar to that reported in adult patients. It is speculated that these age-related differences in host responses are associated with the ontogeny of the immunologic system.

Encephalitis in Infectious Mononucleosis: Diagnostic Considerations

PEDIATRICS ◽  
1976 ◽  
Vol 58 (6) ◽  
pp. 877-880
Author(s):  
Beverly J. Lange ◽  
Peter H. Berman ◽  
Joseph Bender ◽  
Werner Henle ◽  
John F. Hewetson
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Nuclear Antigen ◽  
Diffuse Component ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Antibody Titers ◽  
Epstein Barr ◽  
Etiologic Diagnosis

Four atypical cases of presumed infectious mononucleosis (IM) encephalitis are presented. To establish an etiologic diagnosis, Paul-Bunnell-Davidsohn heterophil titers (PBD), antibody titers to the antigens of the Epstein-Barr virus (EBV), and oropharyngeal excretion of EBV were determined. Criteria for a primary EBV infection are (1) an antiviral capsid antigen titer of 1:160 or greater, (2) the presence of antibody to the diffuse component of the early antigen, (3) absence of antibody to the nuclear antigen, and (4) excretion of the virus from the oropharynx. Three of the four cases met these criteria; of the three, one did not have a positive heterophil titer. The fourth case turned out not to be IM; there was a positive PBD heterophil, but there was no evidence of primary EBV infection. Although the PBD heterophil is usually a reliable test to diagnosis IM, it is not always present in children, and it is sometimes nonspecifically elevated. Some EBV titers can be nonspecifically elevated as well; however, the above criteria are diagnostic of primary EBV infection.

scholarly journals Genotypic Characterization of Epstein Barr Virus in Blood of Patients with Suspected Nasopharyngeal Carcinoma in Ghana

Viruses ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 766
Author(s):  
Richmond Ayee ◽  
Maame Ekua Oforiwaa Ofori ◽  
Emmanuel Ayitey Tagoe ◽  
Sylvester Languon ◽  
Kafui Searyoh ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Venous Blood ◽  
Nasopharyngeal Cancer ◽  
Nuclear Antigen ◽  
Control Group ◽  
Barr Virus ◽  
Genotype 2 ◽  
Epstein Barr ◽  
Study Participants ◽  
Epstein Barr Nuclear Antigen

Nasopharyngeal cancer (NPC) is associated with Epstein Barr virus (EBV) infection. However different viral strains have been implicated in NPC worldwide. This study aimed to detect and characterize EBV in patients diagnosed with NPC in Ghana. A total of 55 patients diagnosed with NPC by CT scan and endoscopy were age-matched with 53 controls without a known oncological disease. Venous blood was collected from the study participants and DNA extracted from the blood samples. Detection of EBV and genotyping were done by amplifying Epstein Barr nuclear antigen 1 (EBNA-1) and Epstein Barr nuclear antigen 2 (EBNA-2), respectively, using specific primers. Viral load in patients and controls was determined using real-time polymerase chain reaction. EBV positivity in controls (92%) was significantly greater than that of NPC patients (67%) (χ2 = 19.17, p < 0.0001), and viral infection was independent of gender (χ2 = 1.770, p = 0.1834). The predominant EBV genotypes in patients and controls were genotype 2 (52%) and genotype 1 (62%), respectively. Median EBV load was significantly higher in NPC patients than the control group (p < 0.01). In summary, prevalence of EBV genotype 2 infection was higher in NPC patients than the control group. Assessment of EBV load may be used as a biomarker for the diagnosis of NPC.

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